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Home / Equine Research Bank / Can J Vet Res / Plasma myeloperoxidase level and polymorphonuclear leukocyte...
Canadian journal of veterinary research = Revue canadienne de recherche veterinaire1999; 63(2); 142-147;

Plasma myeloperoxidase level and polymorphonuclear leukocyte activation in horses suffering from large intestinal obstruction requiring surgery: preliminary results.

Abstract: Myeloperoxidase (MPO) is a specific enzyme of neutrophil azurophilic granules with a strong oxidative activity. Thanks to a radioimmunoassay of equine myeloperoxidase, the authors have observed a significantly higher plasma level of MPO in horses operated for strangulation obstruction of the large intestine (n = 6) than in horses suffering from a non-strangulating displacement of the large intestine (n = 9). For the 2 groups, 3 phases were distinguished: reception (P1), intensive care (P2) and terminal phase (P3). The mean peak values of MPO for these phases were 121.6 ng/mL (P1), 168.6 ng/mL (P2), and 107.0 ng/mL (P3) for the non-strangulating group, and 242.6 ng/mL (P1); 426.0 ng/mL (P2), and 379.5 ng/mL (P3) for the strangulation group. The variations of the mean peak values of plasma MPO were significantly different between the 2 groups and between the different phases. A significant increase of the least square means of MPO was observed between P1 and P2. A significant decrease of the least square means of the number of circulating leukocytes was observed between P1 and P3. Polymorphonuclear neutrophil activation could play a major role in the pathogenesis of acute abdominal disease and endotoxic shock.
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Publication Date: 1999-06-16 PubMed ID: 10369573PubMed Central: PMC1189534
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  • Journal Article
  • Research Support
  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The study investigates the levels of an enzyme called myeloperoxidase (MPO) in horses undergoing surgery for large intestinal obstructions, noting that there is a significantly higher MPO level in those with a more serious obstruction type, which suggests the enzyme plays an important role in disease pathogenesis.

Introduction and Objective

  • The research centers around the higher level of myeloperoxidase (MPO) – a specific enzyme of neutrophil azurophilic granules, linked with strong oxidative activity – in horses who had to undergo surgery due to strangulation obstruction of the large intestine compared to horses suffering from non-strangulating displacement of the large intestine.

Methods

  • Researchers used a radioimmunoassay – a technique that involves the use of radioactive isotopes to ascertain the concentration of specific substances – of equine myeloperoxidase.
  • In the study, they distinguished three phases based on the severity of the condition and stage of treatment: reception (P1), intensive care (P2), and terminal phase (P3).
  • The study included two groups of horses – one with strangulation obstruction of the large intestine (6 horses) and the other with non-strangulating displacement of the large intestine (9 horses).

Results

  • The findings showed significantly different mean peak values of plasma MPO in the two groups: the non-strangulating group had MPO values of 121.6 ng/mL (P1), 168.6 ng/mL (P2), and 107.0 ng/mL (P3), while the strangulating group had much higher MPO values of 242.6 ng/mL (P1), 426.0 ng/mL (P2), and 379.5 ng/mL (P3).
  • Along with these different levels, the study also revealed a significant increase in the least square means of MPO between the reception phase (P1) and the intensive care phase (P2).
  • Moreover, the least square means of the number of circulating leukocytes showed a significant decrease between P1 and P3.

Discussion and Conclusion

  • The findings indicate that the variations of the mean peak values of plasma MPO were significantly different between the two groups and between the different phases.
  • The researchers conclude that the activation of polymorphonuclear neutrophils, a type of white blood cell, might play an integral role in the pathogenesis of acute abdominal disease and endotoxic shock.

Cite This Article

APA
Grulke S, Benbarek H, Caudron I, Deby-Dupont G, Mathy-Hartert M, Farnir F, Deby C, Lamy M, Serteyn D. (1999). Plasma myeloperoxidase level and polymorphonuclear leukocyte activation in horses suffering from large intestinal obstruction requiring surgery: preliminary results. Can J Vet Res, 63(2), 142-147.

Publication

Canadian journal of veterinary research = Revue canadienne de recherche veterinaire
ISSN: 0830-9000
NlmUniqueID: 8607793
Country: Canada
Language: English
Volume: 63
Issue: 2
Pages: 142-147

Researcher Affiliations

Grulke, S
  • Anaesthésiologie générale, Pathologie chirugicale des grands animaux, Université de Liège, Sart Tilman, Belgium.
Benbarek, H
    Caudron, I
      Deby-Dupont, G
        Mathy-Hartert, M
          Farnir, F
            Deby, C
              Lamy, M
                Serteyn, D

                  MeSH Terms

                  • Acute Disease
                  • Animals
                  • Colonic Diseases / physiopathology
                  • Colonic Diseases / surgery
                  • Colonic Diseases / veterinary
                  • Critical Care
                  • Horse Diseases / blood
                  • Horse Diseases / physiopathology
                  • Horse Diseases / surgery
                  • Horses
                  • Intestinal Obstruction / blood
                  • Intestinal Obstruction / physiopathology
                  • Intestinal Obstruction / surgery
                  • Least-Squares Analysis
                  • Leukocyte Count
                  • Neutrophils / physiology
                  • Peroxidase / blood

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                  Citations

                  This article has been cited 3 times.
                  1. Verwilghen DR, Martens A, Busschers E, Franck T, Deberg M, Henrotin Y, Vanderheyden L, Serteyn D. Coll2-1, Coll2-1NO2 and myeloperoxidase concentrations in the synovial fluid of equine tarsocrural joints affected with osteochondrosis.. Vet Res Commun 2011 Oct;35(7):401-8.
                    doi: 10.1007/s11259-011-9487-5pubmed: 21681550google scholar: lookup
                  2. Grulke S, Franck T, Gangl M, Péters F, Salciccia A, Deby-Dupont G, Serteyn D. Myeloperoxidase assay in plasma and peritoneal fluid of horses with gastrointestinal disease.. Can J Vet Res 2008 Jan;72(1):37-42.
                    pubmed: 18214160
                  3. Art T, Franck T, Lekeux P, de Moffarts B, Couëtil L, Becker M, Kohnen S, Deby-Dupont G, Serteyn D. Myeloperoxidase concentration in bronchoalveolar lavage fluid from healthy horses and those with recurrent airway obstruction.. Can J Vet Res 2006 Oct;70(4):291-6.
                    pubmed: 17042382
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