Plasma Non-targeted Metabolomics Analysis of Yili Horses Raced on Tracks With Different Surface Hardness.

The research studies the change in biochemical substances in a breed of horses, Yili, during exercise on differing levels of surface hardness. It suggests Vitamin C and methylmalonic acid as potential novel markers for equine locomotion injury.

Objective of the Study

• The main objective of the study was to understand the biochemical changes in Yili horses during exercise.
• This was achieved through a thorough analysis of the horses’ plasma metabolomics pre and post exercise on surfaces with varying hardness.
• The study also aimed to offer further insights into equine exercise physiology.

Methodology

• The researchers collected blood samples from eight trained Yili horses, both prior to and immediately after the exercise.
• For a comprehensive metabolomics analysis, they used ultra-performance liquid chromatography-Q-EXACTIVE mass spectrometry, a highly sensitive and widely-used technique in metabolite profiling.

Key Findings

• The analysis revealed 938 significantly altered metabolites, particularly in sugar, lipid, and amino acid metabolism.
• Among them, significant increases were detected in glucose, glucoheptanoic acid, lactic acid, malic acid, and methylmalonic acid post the exercise.
• In contrast, notable reductions were observed in creatinine, D-tryptophan, carnitine, and citric acid.
• The study also mentions specific metabolites such as acetylcarnitine, tuliposide, vitamin C, and methylmalonic acid. The concentration of these metabolites showed a regular pattern of change post exercise on surfaces of various hardness levels.

Significance

• This research provides valuable insights into equine exercise physiology, making notable contributions to the field.
• Two metabolites, vitamin C and methylmalonic acid, were identified as potential new biomarkers for equine locomotion injuries. This finding indicates potential for recognizing and diagnosing locomotion problems in horses at an early stage.