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The Canadian veterinary journal = La revue veterinaire canadienne2015; 56(2); 178-184;

Plasma serotonin in horses undergoing surgery for small intestinal colic.

Abstract: This study compared serotonin concentrations in platelet poor plasma (PPP) from healthy horses and horses with surgical small intestinal (SI) colic, and evaluated their association with postoperative ileus, strangulation and non-survival. Plasma samples (with EDTA) from 33 horses with surgical SI colic were collected at several pre- and post-operative time points. Serotonin concentrations were determined using liquid-chromatography tandem mass spectrometry. Results were compared with those for 24 healthy control animals. The serotonin concentrations in PPP were significantly lower (P < 0.01) in pre- and post-operative samples from surgical SI colic horses compared to controls. However, no association with postoperative ileus or non-survival could be demonstrated at any time point. In this clinical study, plasma serotonin was not a suitable prognostic factor in horses with SI surgical colic. Sérotonine plasmatique chez des chevaux subissant une chirurgie pour des coliques du petit intestin. Cette étude a comparé les concentrations de sérotonine dans le plasma faible en plaquettes (PFP) de chevaux en santé et de chevaux atteints de coliques chirurgicales du petit intestin et a évalué leur association avec l’occlusion intestinale postopératoire, la strangulation et la non-survie. Des échantillons de plasma (avec EDTA) ont été prélevés auprès de 33 chevaux atteints de coliques du petit intestin à plusieurs moments préopératoires et postopératoires. Les concentrations de sérotonine ont été déterminées à l’aide d’un spectromètre de masse LC-ESI-MS/MS. Les résultats ont été comparés avec ceux de 24 animaux témoins en santé. Les concentrations de sérotonine du PFP étaient significativement inférieures (P < 0,01) dans les échantillons préopératoires et postopératoires provenant des chevaux atteints de coliques du petit intestin comparativement aux animaux témoins. Cependant, aucune association avec l’occlusion intestinale postopératoire ou la non-survie n’a pu être démontrée à aucun moment. Dans cette étude clinique, la sérotonine plasmatique ne s’est pas avéré un facteur de pronostic approprié chez les chevaux atteints de coliques chirurgicales du petit intestin.(Traduit par Isabelle Vallières).
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Publication Date: 2015-02-20 PubMed ID: 25694668PubMed Central: PMC4298271
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  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research aimed to evaluate the concentration of serotonin in the plasma of healthy horses compared to horses undergoing surgery for small intestinal colic and to assess its relation to postoperative complications. The findings did not identify plasma serotonin as a suitable prognostic indicator for such cases.

Research Purpose and Methodology

  • The primary aim of this study was to compare the serotonin levels in the plasma of healthy horses to those in horses undergoing surgery for small intestinal colic. The researchers also aimed to investigate whether these serotonin concentrations were associated with postoperative complications, specifically ileus (intestinal obstruction) and non-survival.
  • Plasma samples were collected from 33 horses that showed surgical small intestinal colic symptoms, at several time points before and after surgery. The plasma was treated with EDTA, a substance that reduces coagulation, to prepare platelet poor plasma (PPP).
  • The researchers used liquid-chromatography tandem mass spectrometry, a technique that combines the physical separation capabilities of liquid chromatography with the mass analysis capabilities of mass spectrometry, to determine serotonin concentrations in the samples.
  • The results obtained from these horses were then compared with data from a control group of 24 healthy horses.

Key Findings

  • The study found that serotonin concentrations in the plasma of horses undergoing surgery for small intestinal colic were significantly lower than those in healthy horses.
  • This was true for both the pre-operative and post-operative samples taken from the surgical SI colic horses when compared to the control group.
  • However, despite this contrast, the researchers found no link between the serotonin concentrations in horses with SI surgical colic and postoperative ileus or non-survival.

Conclusion

  • The findings from this study do not support the assumption that plasma serotonin concentrations could serve as a reliable prognostic factor in horses undergoing surgery for small intestinal colic.
  • This means clinicians cannot use plasma serotonin levels to forecast the likelihood of postoperative ileus, strangulation, or non-survival in horses presenting surgical SI colic.

Cite This Article

APA
Torfs SC, Maes AA, Delesalle CJ, Pardon B, Croubels SM, Deprez P. (2015). Plasma serotonin in horses undergoing surgery for small intestinal colic. Can Vet J, 56(2), 178-184.

Publication

The Canadian veterinary journal = La revue veterinaire canadienne
ISSN: 0008-5286
NlmUniqueID: 0004653
Country: Canada
Language: English
Volume: 56
Issue: 2
Pages: 178-184

Researcher Affiliations

Torfs, Sara C
  • Department of Large Animal Internal Medicine and Clinical Biology (Torfs, Delesalle, Pardon, Deprez), Department of Pharmacology, Toxicology and Biochemistry (Maes, Croubels), Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, B-9820, Merelbeke, Belgium; Department of Equine Sciences, Faculty of Veterinary Medicine, Utrecht University, Yalelaan 1, NL-3584 CL, Utrecht, The Netherlands (Delesalle).
Maes, An A
  • Department of Large Animal Internal Medicine and Clinical Biology (Torfs, Delesalle, Pardon, Deprez), Department of Pharmacology, Toxicology and Biochemistry (Maes, Croubels), Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, B-9820, Merelbeke, Belgium; Department of Equine Sciences, Faculty of Veterinary Medicine, Utrecht University, Yalelaan 1, NL-3584 CL, Utrecht, The Netherlands (Delesalle).
Delesalle, Catherine J
  • Department of Large Animal Internal Medicine and Clinical Biology (Torfs, Delesalle, Pardon, Deprez), Department of Pharmacology, Toxicology and Biochemistry (Maes, Croubels), Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, B-9820, Merelbeke, Belgium; Department of Equine Sciences, Faculty of Veterinary Medicine, Utrecht University, Yalelaan 1, NL-3584 CL, Utrecht, The Netherlands (Delesalle).
Pardon, Bart
  • Department of Large Animal Internal Medicine and Clinical Biology (Torfs, Delesalle, Pardon, Deprez), Department of Pharmacology, Toxicology and Biochemistry (Maes, Croubels), Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, B-9820, Merelbeke, Belgium; Department of Equine Sciences, Faculty of Veterinary Medicine, Utrecht University, Yalelaan 1, NL-3584 CL, Utrecht, The Netherlands (Delesalle).
Croubels, Siska M
  • Department of Large Animal Internal Medicine and Clinical Biology (Torfs, Delesalle, Pardon, Deprez), Department of Pharmacology, Toxicology and Biochemistry (Maes, Croubels), Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, B-9820, Merelbeke, Belgium; Department of Equine Sciences, Faculty of Veterinary Medicine, Utrecht University, Yalelaan 1, NL-3584 CL, Utrecht, The Netherlands (Delesalle).
Deprez, Piet
  • Department of Large Animal Internal Medicine and Clinical Biology (Torfs, Delesalle, Pardon, Deprez), Department of Pharmacology, Toxicology and Biochemistry (Maes, Croubels), Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, B-9820, Merelbeke, Belgium; Department of Equine Sciences, Faculty of Veterinary Medicine, Utrecht University, Yalelaan 1, NL-3584 CL, Utrecht, The Netherlands (Delesalle).

MeSH Terms

  • Animals
  • Colic / blood
  • Colic / pathology
  • Colic / surgery
  • Colic / veterinary
  • Female
  • Horse Diseases / blood
  • Horse Diseases / surgery
  • Horses
  • Intestine, Small / pathology
  • Male
  • Platelet Activation
  • Serotonin / blood
  • Serotonin / metabolism

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Citations

This article has been cited 1 times.
  1. Salavati S, Mogheiseh A, Nazifi S, Amiri A, Nikahval B. The effects of melatonin on the concentrations of inflammatory cytokines and proteins, serotonin, cortisol and melatonin in ovariohysterectomised female dogs. Vet Med Sci 2023 May;9(3):1103-1113.
    doi: 10.1002/vms3.1112pubmed: 36913177google scholar: lookup
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