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Home / Equine Research Bank / Vet Med Int / Platelet-Rich Gel Supernatants Stimulate the Release of Anti...
Veterinary medicine international2015; 2015; 547052; doi: 10.1155/2015/547052

Platelet-Rich Gel Supernatants Stimulate the Release of Anti-Inflammatory Proteins on Culture Media of Normal Equine Synovial Membrane Explants.

Abstract: The aims were as follows: (1) to evaluate the effects at 48 and 96 h of two concentrations (25 and 50%) of leukocyte and platelet-rich gel (L-PRG) and pure PRG (P-PRG) supernatants on the production/degradation in normal equine synovial membrane explants (SME) of platelet derived growth factor isoform BB, transforming growth factor beta-1, tumor necrosis factor alpha, interleukin (IL-) 4 (IL-4), IL-1 receptor antagonist (IL-1ra), and hyaluronan (HA) synthesis and (2) to correlate these molecules with their respective PRG supernatant treatments. SME from 6 horses were cultured for 96 h with L-PRG and P-PRG supernatants at 25 and 50% concentrations, respectively. SME culture media were changed each 48 h and used for determination by ELISA of the molecules, which were also determined in synovial fluid. 25% L-PRG supernatant produced a sustained release over time of IL-1ra and a gradual release of HA, whereas 50% L-PRG supernatant produced a sustained increase over time of IL-4 and HA. 50% P-PRG supernatant produced an increased and sustained production of IL-1ra and IL-4. The cellular composition and the articular concentration (volume) of a platelet-rich plasma preparation could affect the anti-inflammatory and anabolic joint responses in horses with osteoarthritis.
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Publication Date: 2015-05-18 PubMed ID: 26090267PubMed Central: PMC4451761DOI: 10.1155/2015/547052Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This study investigates how different concentrations of leukocyte and platelet-rich gel (L-PRG) and pure platelet-rich gel (P-PRG) affect the production of various proteins and molecules in the synovial membrane explants of horses. These proteins include growth factors, anti-inflammatory agents and other compounds that can impact inflammation and cartilage degeneration associated with osteoarthritis.

Objective and Methodology of the Study

  • The main objective of the research was to understand how different concentrations of L-PRG and P-PRG supernatants impact the production or degradation of various proteins in horse synovial membrane explants.
  • The proteins under consideration were platelet-derived growth factor isoform BB, transforming growth factor beta-1, tumor necrosis factor alpha, interleukin (IL) 4, IL-1 receptor antagonist (IL-1ra), and hyaluronan (HA) – all of which play significant roles in inflammation and cartilage regeneration.
  • The synovial membrane explants were taken from 6 horses and were cultured for 96 hours with 25% and 50% concentrations of both L-PRG and P-PRG supernatants.
  • The culture media were refreshed every 48 hours, and at these time points, the aforementioned proteins were measured using ELISA.

Results of the Study

  • The findings indicated that when cultured with 25% L-PRG supernatant, IL-1ra (an anti-inflammatory protein) was released over time steadily, and the production of HA (a compound integral for cartilage health) was observed gradually.
  • On the other hand, 50% L-PRG supernatant induced a consistent increased release of IL-4 (another anti-inflammatory protein) and HA.
  • When subjected to 50% P-PRG supernatant, a sustained increase in the production of IL-1ra and IL-4 was noted.
  • These findings suggest that different concentrations of L-PRG and P-PRG could significantly influence the anti-inflammatory and anabolic responses in horse joints, potentially impacting the management of osteoarthritis in these animals.

Implication of the Study

  • The study sheds light on how different concentrations of platelet-rich plasma preparations could affect the inflammatory reactions and joint health in horses suffering from osteoarthritis.
  • It underlines the potential effectiveness of these treatments in managing osteoarthritis and could pave the way for further research in this direction.

Cite This Article

APA
Ríos DL, López C, Carmona JU. (2015). Platelet-Rich Gel Supernatants Stimulate the Release of Anti-Inflammatory Proteins on Culture Media of Normal Equine Synovial Membrane Explants. Vet Med Int, 2015, 547052. https://doi.org/10.1155/2015/547052

Publication

Veterinary medicine international
ISSN: 2090-8113
NlmUniqueID: 101524203
Country: United States
Language: English
Volume: 2015
Pages: 547052

Researcher Affiliations

Ríos, Diana L
  • Grupo de Investigación Terapia Regenerativa, Departamento de Salud Animal, Universidad de Caldas, Calle 65 No. 26-10, Manizales, Colombia.
López, Catalina
  • Grupo de Investigación Terapia Regenerativa, Departamento de Salud Animal, Universidad de Caldas, Calle 65 No. 26-10, Manizales, Colombia.
Carmona, Jorge U
  • Grupo de Investigación Terapia Regenerativa, Departamento de Salud Animal, Universidad de Caldas, Calle 65 No. 26-10, Manizales, Colombia.

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