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Journal of orthopaedic research : official publication of the Orthopaedic Research Society2006; 25(2); 230-240; doi: 10.1002/jor.20278

Platelet rich plasma (PRP) enhances anabolic gene expression patterns in flexor digitorum superficialis tendons.

Abstract: Platelet rich plasma (PRP) has recently been investigated for use in tissue regeneration studies that seek to utilize the numerous growth factors released from platelet alpha-granules. This study examined gene expression patterns, DNA, and collagen content of equine flexor digitorum superficialis tendon (SDFT) explants cultured in media consisting of PRP and other blood products. Blood and bone marrow aspirate (BMA) were collected from horses and processed to obtain plasma, PRP, and platelet poor plasma (PPP). IGF-I, TGF-beta1, and PDGF-BB were quantified in all blood products using ELISA. Tendons were cultured in explant fashion with blood, plasma, PRP, PPP, or BMA at concentrations of 100%, 50%, or 10% in serum-free DMEM with amino acids. Quantitative RT-PCR for expression of collagen type I (COL1A1), collagen type III (COL3A1), cartilage oligomeric matrix protein (COMP), decorin, matrix metalloproteinase-3 (MMP-3), and matrix metalloproteinase-13 (MMP-13) was performed as were DNA and total soluble collagen assays. TGF-beta1 and PDGF-BB concentrations were higher in PRP compared to all other blood products tested. Tendons cultured in 100% PRP showed enhanced gene expression of the matrix molecules COL1A1, COL3A1, and COMP with no concomitant increase in the catabolic molecules MMP-3 and MMP-13. These findings support in vivo investigation of PRP as an autogenous, patient-side treatment for tendonitis.
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Publication Date: 2006-11-16 PubMed ID: 17106885DOI: 10.1002/jor.20278Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research investigated how platelet rich plasma (PRP) can enhance the expressions of anabolic genes in equine flexor digitorum superficialis tendons, potentially supporting PRP’s use in treating tendonitis.

Introduction and Methodology

  • This investigation focused on the use of Platelet-Rich Plasma (PRP) in tissue regeneration, leveraging the rich supply of growth factors released from platelet alpha granules. PRP, along with other blood products, were examined in relation to the gene expression patterns, DNA and collagen content of equine flexor digitorum superficialis tendon (SDFT) explants.
  • Aside from PRP, other blood derivatives like plasma, platelet poor plasma (PPP), and bone marrow aspirate (BMA) were harvested from the horses. These were prepared in a medium of serum-free DMEM with amino acids.
  • The research quantified the presence of growth factors IGF-I, TFG-beta1, and PDGF-BB in all the tested blood products using the Enzyme-Linked Immunosorbent Assay (ELISA) technique.
  • Finally, the tendons were cultured in explant fashion with these blood products at varying concentrations namely 100%, 50%, and 10%.

Key Findings

  • The results revealed that TGF-beta1 and PDGF-BB concentrations were higher in PRP when compared to the other blood products tested.
  • The tendons cultured in 100% PRP exhibited an elevated gene expression of matrix molecules like collagen type I (COL1A1), collagen type III (COL3A1), and cartilage oligomeric matrix protein (COMP).
  • Interestingly, the enhanced gene expression did not cause an increase in catabolic molecules such as matrix metalloproteinase-3 (MMP-3) and matrix metalloproteinase-13 (MMP-13).
  • This supports the anabolic (building) rather than catabolic (breaking down) effect of PRP on tendon tissues.

Significance of the Research

  • The findings from this research provide a promising foundation for further in vivo investigations of PRP as a patient-side treatment for tendonitis.
  • The elevated gene expression of key matrix molecules in the presence of PRP suggests that PRP application may assist in the therapeutic recovery of damaged tissues in tendonitis.

Overall, the application of PRP appears to offer beneficial effects that support tissue regeneration and recovery, specifically in the context of tendon injuries, paving the way for subsequent studies on the potential use of PRP in healing and recovery modalities.

Cite This Article

APA
Schnabel LV, Mohammed HO, Miller BJ, McDermott WG, Jacobson MS, Santangelo KS, Fortier LA. (2006). Platelet rich plasma (PRP) enhances anabolic gene expression patterns in flexor digitorum superficialis tendons. J Orthop Res, 25(2), 230-240. https://doi.org/10.1002/jor.20278

Publication

Journal of orthopaedic research : official publication of the Orthopaedic Research Society
ISSN: 0736-0266
NlmUniqueID: 8404726
Country: United States
Language: English
Volume: 25
Issue: 2
Pages: 230-240

Researcher Affiliations

Schnabel, Lauren V
  • Department of Clinical Sciences, VMC C3-181, Cornell University, Ithaca, New York 14853, USA.
Mohammed, Hussni O
    Miller, Brian J
      McDermott, William G
        Jacobson, May S
          Santangelo, Kelly S
            Fortier, Lisa A

              MeSH Terms

              • Animals
              • Becaplermin
              • Collagen / genetics
              • Collagen / metabolism
              • DNA / genetics
              • DNA / metabolism
              • Dose-Response Relationship, Drug
              • Extracellular Matrix Proteins / genetics
              • Extracellular Matrix Proteins / metabolism
              • Gene Expression Regulation / physiology
              • Glycoproteins / genetics
              • Glycoproteins / metabolism
              • Horses
              • Insulin-Like Growth Factor I / genetics
              • Insulin-Like Growth Factor I / metabolism
              • Matrilin Proteins
              • Metabolism / genetics
              • Metabolism / physiology
              • Platelet-Derived Growth Factor / genetics
              • Platelet-Derived Growth Factor / metabolism
              • Platelet-Rich Plasma / physiology
              • Proto-Oncogene Proteins c-sis
              • RNA, Messenger / genetics
              • RNA, Messenger / metabolism
              • Tendinopathy / drug therapy
              • Tendons / metabolism
              • Tissue Culture Techniques
              • Transforming Growth Factor beta1 / genetics
              • Transforming Growth Factor beta1 / metabolism

              Citations

              This article has been cited 104 times.
              1. Carmona JU, López C, Argüelles D. Addressing Heterogeneity in Equine PRP Therapies: A Scoping Review of Methods, Evidence, and Commercial Validation. Animals (Basel) 2025 Dec 13;15(24).
                doi: 10.3390/ani15243586pubmed: 41463871google scholar: lookup
              2. Aboelsaad E, Moustafa S, Amine A, Deghady A, El-Attar L. Platelet-rich plasma as a potential antimicrobial agent against multidrug-resistant bacteria in diabetic foot infections. Sci Rep 2025 Apr 30;15(1):15145.
                doi: 10.1038/s41598-025-97418-0pubmed: 40307308google scholar: lookup
              3. Yang M, Deng B, Hao W, Jiang X, Chen Y, Wang M, Yuan Y, Chen M, Wu X, Du C, Armstrong DG, Guo L, Deng W, Wang H. Platelet concentrates in diabetic foot ulcers: A comparative review of PRP, PRF, and CGF with case insights. Regen Ther 2025 Mar;28:625-632.
                doi: 10.1016/j.reth.2025.02.005pubmed: 40166040google scholar: lookup
              4. Michalczik F, Trieb K. [Treatment of rotator cuff lesions using autologous conditioned plasma-a systematic review]. Orthopadie (Heidelb) 2025 Apr;54(4):294-301.
                doi: 10.1007/s00132-025-04632-9pubmed: 40056180google scholar: lookup
              5. García-Bordes L, Álvarez-Díaz P, Alentorn-Geli E, Ferré-Aniorte A, Laiz-Boada P, Seijas-Vázquez R, Cugat-Bertomeu R. Demographic, anthropometric and intrasubject variations affect platelet-rich plasma formulation. J Exp Orthop 2025 Jan;12(1):e70024.
                doi: 10.1002/jeo2.70024pubmed: 39867676google scholar: lookup
              6. Katz NB, Tsitsilianos N, Nowak AS, Douglas SR, Tenforde AS, Borg-Stein J. Advanced Non-Operative Interventions for Anterior Knee Pain. Curr Rev Musculoskelet Med 2024 Dec;17(12):589-615.
                doi: 10.1007/s12178-024-09930-xpubmed: 39527393google scholar: lookup
              7. Schepers MO, Groot D, Kleinjan EM, Pol MM, Mylenbusch H, Klopper-Kes AHJ. Effectiveness of intradiscal platelet rich plasma for discogenic low back pain without Modic changes: A randomized controlled trial. Interv Pain Med 2022 Mar;1(1):100011.
                doi: 10.1016/j.inpm.2022.100011pubmed: 39238810google scholar: lookup
              8. Lunardon T, Sumner SM, Mollabashi M, Darzenta N, Davis E, Naskou MC. Growth factor and cytokine characterization of canine platelet lysate with variable leukocyte concentration, plasma content, and heat-sensitive proteins. Front Vet Sci 2024;11:1408080.
                doi: 10.3389/fvets.2024.1408080pubmed: 39071789google scholar: lookup
              9. Cl K, Jeyaraman M, Jeyaraman N, Ramasubramanian S, Khanna M, Yadav S. Antimicrobial Effects of Platelet-Rich Plasma and Platelet-Rich Fibrin: A Scoping Review. Cureus 2023 Dec;15(12):e51360.
                doi: 10.7759/cureus.51360pubmed: 38292974google scholar: lookup
              10. Albahrawy M, Abouelnasr K, Mosbah E, Zaghloul A, Abass M. Biostimulation effect of platelet-rich fibrin augmented with decellularized bovine pericardium on full-thickness cutaneous wound healing in Donkeys (Equus asinus). BMC Vet Res 2023 Sep 20;19(1):166.
                doi: 10.1186/s12917-023-03733-xpubmed: 37730587google scholar: lookup
              11. Disantis A, Andrade AJ, Baillou A, Bonin N, Byrd T, Campbell A, Domb B, Doyle H, Enseki K, Getz B, Gosling L, Grant L, M Ilizaliturri V Jr, Kohlrieser D, Laskovski J, Lifshitz L, P McGovern R, Monnington K, O'Donnell J, Takla A, Tyler T, Voight M, Wuerz T, Martin RL. The 2022 International Society for Hip Preservation (ISHA) physiotherapy agreement on assessment and treatment of greater trochanteric pain syndrome (GTPS): an international consensus statement. J Hip Preserv Surg 2023 Jan;10(1):48-56.
                doi: 10.1093/jhps/hnac050pubmed: 37275836google scholar: lookup
              12. Xu J, Du W, Xue X, Chen M, Zhou W, Luo X. Global research trends on platelet-rich plasma for tendon and ligament injuries from the past two decades: A bibliometric and visualized study. Front Surg 2023;10:1113491.
                doi: 10.3389/fsurg.2023.1113491pubmed: 36843990google scholar: lookup
              13. Chalidis B, Givissis P, Papadopoulos P, Pitsilos C. Molecular and Biologic Effects of Platelet-Rich Plasma (PRP) in Ligament and Tendon Healing and Regeneration: A Systematic Review. Int J Mol Sci 2023 Feb 1;24(3).
                doi: 10.3390/ijms24032744pubmed: 36769065google scholar: lookup
              14. Liu X, Li Y, Shen L, Yan M. Leukocyte and Platelet-Rich Plasma (L-PRP) in Tendon Models: A Systematic Review and Meta-Analysis of in vivo/in vitro Studies. Evid Based Complement Alternat Med 2022;2022:5289145.
                doi: 10.1155/2022/5289145pubmed: 36569346google scholar: lookup
              15. Albahrawy M, Abouelnasr K, Mosbah E, Zaghloul A, Abass M. Acellular bovine pericardium as a biological dressing for treatment of cutaneous wounds of the distal limb in donkeys (Equus Asinus). Vet Res Commun 2023 Jun;47(2):587-597.
                doi: 10.1007/s11259-022-10014-9pubmed: 36323838google scholar: lookup
              16. Cavallo C, Boffa A, de Girolamo L, Merli G, Kon E, Cattini L, Santo E, Grigolo B, Filardo G. Bone marrow aspirate concentrate quality is affected by age and harvest site. Knee Surg Sports Traumatol Arthrosc 2023 Jun;31(6):2140-2151.
                doi: 10.1007/s00167-022-07153-6pubmed: 36156111google scholar: lookup
              17. Angelou V, Psalla D, Dovas CI, Kazakos GM, Marouda C, Chatzimisios K, Kyrana Z, Moutou E, Karayannopoulou M, Papazoglou LG. Locally Injected Autologous Platelet-Rich Plasma Improves Cutaneous Wound Healing in Cats. Animals (Basel) 2022 Aug 6;12(15).
                doi: 10.3390/ani12151993pubmed: 35953982google scholar: lookup
              18. Arliani GG, Durigon TS, Pedroso JP, Ferreira GF, Oksman D, Oliveira VO. Intra-articular Infiltration of Platelet-Rich Plasma versus Hyaluronic Acid in Patients with Primary Knee Osteoarthritis: Preliminary Results from a Randomized Clinical Trial. Rev Bras Ortop (Sao Paulo) 2022 Jun;57(3):402-408.
                doi: 10.1055/s-0041-1724082pubmed: 35785122google scholar: lookup
              19. Bugarin A, Schroeder G, Shi BY, Jones KJ, Kremen TJ Jr. Assessment of Characteristics and Methodological Quality of the Top 50 Most Cited Articles on Platelet-Rich Plasma in Musculoskeletal Medicine. Orthop J Sports Med 2022 May;10(5):23259671221093074.
                doi: 10.1177/23259671221093074pubmed: 35656192google scholar: lookup
              20. Viganò M, Ragni E, Marmotti A, de Girolamo L. The effects of orthobiologics in the treatment of tendon pathologies: a systematic review of preclinical evidence. J Exp Orthop 2022 Apr 8;9(1):31.
                doi: 10.1186/s40634-022-00468-wpubmed: 35394237google scholar: lookup
              21. Evans JP, Maffulli N, Smith C, Watts A, Valderas J, Goodwin V. Even experts cannot agree on the optimal use of platelet-rich plasma in lateral elbow tendinopathy: an international Delphi study. J Orthop Traumatol 2021 Nov 25;22(1):47.
                doi: 10.1186/s10195-021-00608-5pubmed: 34825302google scholar: lookup
              22. Oliveira-Filho MA, Souza M, Tabushi FI, Almeida LE, Figueiredo FP, Lourenço ES, Giovanini AF, Malafaia O, Ribas Filho JM. Platelet-concentrated and platelet poor-plasma promote different pattern on immunohistochemical expression of TGF-β1, however they impairs the osteoneogensis in calvarial defects treated with autograft due suppression of osteocalcin. Acta Cir Bras 2021;36(6):e360604.
                doi: 10.1590/ACB360604pubmed: 34287607google scholar: lookup
              23. Maleas G, Mageed M. Effectiveness of Platelet-Rich Plasma and Bone Marrow Aspirate Concentrate as Treatments for Chronic Hindlimb Proximal Suspensory Desmopathy. Front Vet Sci 2021;8:678453.
                doi: 10.3389/fvets.2021.678453pubmed: 34222402google scholar: lookup
              24. Montano C, Auletta L, Greco A, Costanza D, Coluccia P, Del Prete C, Meomartino L, Pasolini MP. The Use of Platelet-Rich Plasma for Treatment of Tenodesmic Lesions in Horses: A Systematic Review and Meta-Analysis of Clinical and Experimental Data. Animals (Basel) 2021 Mar 12;11(3).
                doi: 10.3390/ani11030793pubmed: 33809227google scholar: lookup
              25. Sabater AL, Mousa HM, Quinones X, Valenzuela F, Sanchez Avila RM, Orive G, Anitua E, Merayo J, Perez VL. Use of autologous plasma rich in growth factors fibrin membrane in the surgical management of ocular surface diseases. Int Ophthalmol 2021 Jul;41(7):2347-2358.
                doi: 10.1007/s10792-021-01788-zpubmed: 33745034google scholar: lookup
              26. Ribitsch I, Oreff GL, Jenner F. Regenerative Medicine for Equine Musculoskeletal Diseases. Animals (Basel) 2021 Jan 19;11(1).
                doi: 10.3390/ani11010234pubmed: 33477808google scholar: lookup
              27. Evans AG, Ivanic MG, Botros MA, Pope RW, Halle BR, Glassman GE, Genova R, Al Kassis S. Rejuvenating the periorbital area using platelet-rich plasma: a systematic review and meta-analysis. Arch Dermatol Res 2021 Nov;313(9):711-727.
                doi: 10.1007/s00403-020-02173-zpubmed: 33433716google scholar: lookup
              28. Tandulwadkar S, Karthick MS. Combined Use of Autologous Bone Marrow-derived Stem Cells and Platelet-rich Plasma for Ovarian Rejuvenation in Poor Responders. J Hum Reprod Sci 2020 Jul-Sep;13(3):184-190.
                doi: 10.4103/jhrs.JHRS_130_19pubmed: 33311903google scholar: lookup
              29. Ribitsch I, Baptista PM, Lange-Consiglio A, Melotti L, Patruno M, Jenner F, Schnabl-Feichter E, Dutton LC, Connolly DJ, van Steenbeek FG, Dudhia J, Penning LC. Large Animal Models in Regenerative Medicine and Tissue Engineering: To Do or Not to Do. Front Bioeng Biotechnol 2020;8:972.
                doi: 10.3389/fbioe.2020.00972pubmed: 32903631google scholar: lookup
              30. Neph A, Schroeder A, Enseki KR, Everts PA, Wang JH, Onishi K. Role of Mechanical Loading for Platelet-Rich Plasma-Treated Achilles Tendinopathy. Curr Sports Med Rep 2020 Jun;19(6):209-216.
                doi: 10.1249/JSR.0000000000000719pubmed: 32516191google scholar: lookup
              31. Begkas D, Chatzopoulos ST, Touzopoulos P, Balanika A, Pastroudis A. Ultrasound-guided Platelet-rich Plasma Application Versus Corticosteroid Injections for the Treatment of Greater Trochanteric Pain Syndrome: A Prospective Controlled Randomized Comparative Clinical Study. Cureus 2020 Jan 7;12(1):e6583.
                doi: 10.7759/cureus.6583pubmed: 32051796google scholar: lookup
              32. Wei L, Wu S, Kuss M, Jiang X, Sun R, Reid P, Qin X, Duan B. 3D printing of silk fibroin-based hybrid scaffold treated with platelet rich plasma for bone tissue engineering. Bioact Mater 2019 Dec;4:256-260.
                doi: 10.1016/j.bioactmat.2019.09.001pubmed: 31667442google scholar: lookup
              33. McClain AK, McCarrel TM. The effect of four different freezing conditions and time in frozen storage on the concentration of commonly measured growth factors and enzymes in equine platelet-rich plasma over six months. BMC Vet Res 2019 Aug 14;15(1):292.
                doi: 10.1186/s12917-019-2040-4pubmed: 31412868google scholar: lookup
              34. Wessely-Szponder J, Szponder T, Bobowiec R, Michalska J. Changes in the Activity of Ovine Blood-derived Macrophages Stimulated with Antimicrobial Peptide Extract (AMP) or Platelet-rich Plasma (PRP). J Vet Res 2019 Jun;63(2):235-242.
                doi: 10.2478/jvetres-2019-0021pubmed: 31276063google scholar: lookup
              35. Wang Y, Han C, Hao J, Ren Y, Wang J. Efficacy of platelet-rich plasma injections for treating Achilles tendonitis : Systematic review of high-quality randomized controlled trials. Orthopade 2019 Sep;48(9):784-791.
                doi: 10.1007/s00132-019-03711-ypubmed: 30937491google scholar: lookup
              36. Sfakianoudis K, Simopoulou M, Nitsos N, Rapani A, Pappas A, Pantou A, Chronopoulou M, Deligeoroglou E, Koutsilieris M, Pantos K. Autologous Platelet-Rich Plasma Treatment Enables Pregnancy for a Woman in Premature Menopause. J Clin Med 2018 Dec 20;8(1).
                doi: 10.3390/jcm8010001pubmed: 30577435google scholar: lookup
              37. Ali M, Oderuth E, Atchia I, Malviya A. The use of platelet-rich plasma in the treatment of greater trochanteric pain syndrome: a systematic literature review. J Hip Preserv Surg 2018 Aug;5(3):209-219.
                doi: 10.1093/jhps/hny027pubmed: 30393547google scholar: lookup
              38. Klatte-Schulz F, Schmidt T, Uckert M, Scheffler S, Kalus U, Rojewski M, Schrezenmeier H, Pruss A, Wildemann B. Comparative Analysis of Different Platelet Lysates and Platelet Rich Preparations to Stimulate Tendon Cell Biology: An In Vitro Study. Int J Mol Sci 2018 Jan 10;19(1).
                doi: 10.3390/ijms19010212pubmed: 29320421google scholar: lookup
              39. Seabaugh KA, Thoresen M, Giguère S. Extracorporeal Shockwave Therapy Increases Growth Factor Release from Equine Platelet-Rich Plasma In Vitro. Front Vet Sci 2017;4:205.
                doi: 10.3389/fvets.2017.00205pubmed: 29270410google scholar: lookup
              40. Gutiérrez CM, López C, Giraldo CE, Carmona JU. Study of a Two-Step Centrifugation Protocol for Concentrating Cells and Growth Factors in Bovine Platelet-Rich Plasma. Vet Med Int 2017;2017:1950401.
                doi: 10.1155/2017/1950401pubmed: 29214094google scholar: lookup
              41. Xiong G, Lingampalli N, Koltsov JCB, Leung LL, Bhutani N, Robinson WH, Chu CR. Men and Women Differ in the Biochemical Composition of Platelet-Rich Plasma. Am J Sports Med 2018 Feb;46(2):409-419.
                doi: 10.1177/0363546517740845pubmed: 29211968google scholar: lookup
              42. Fukuda K, Miyata H, Kuwano A, Kuroda T, Tamura N, Kotoyori Y, Kasashima Y. Does the injection of platelet-rich plasma induce changes in the gene expression and morphology of intact Thoroughbred skeletal muscle?. J Equine Sci 2017;28(2):31-39.
                doi: 10.1294/jes.28.31pubmed: 28721121google scholar: lookup
              43. Shin HS, Woo HM, Kang BJ. Optimisation of a double-centrifugation method for preparation of canine platelet-rich plasma. BMC Vet Res 2017 Jun 26;13(1):198.
                doi: 10.1186/s12917-017-1123-3pubmed: 28651609google scholar: lookup
              44. Sanchez-Avila RM, Merayo-Lloves J, Riestra AC, Fernandez-Vega Cueto L, Anitua E, Begoña L, Muruzabal F, Orive G. Treatment of patients with neurotrophic keratitis stages 2 and 3 with plasma rich in growth factors (PRGF-Endoret) eye-drops. Int Ophthalmol 2018 Jun;38(3):1193-1204.
                doi: 10.1007/s10792-017-0582-7pubmed: 28620707google scholar: lookup
              45. Chahla J, Mannava S, Cinque ME, Geeslin AG, Codina D, LaPrade RF. Bone Marrow Aspirate Concentrate Harvesting and Processing Technique. Arthrosc Tech 2017 Apr;6(2):e441-e445.
                doi: 10.1016/j.eats.2016.10.024pubmed: 28580265google scholar: lookup
              46. Zhang JY, Fabricant PD, Ishmael CR, Wang JC, Petrigliano FA, Jones KJ. Utilization of Platelet-Rich Plasma for Musculoskeletal Injuries: An Analysis of Current Treatment Trends in the United States. Orthop J Sports Med 2016 Dec;4(12):2325967116676241.
                doi: 10.1177/2325967116676241pubmed: 28210648google scholar: lookup
              47. Iyer SR, Xu S, Stains JP, Bennett CH, Lovering RM. Superparamagnetic Iron Oxide Nanoparticles in Musculoskeletal Biology. Tissue Eng Part B Rev 2017 Aug;23(4):373-385.
                doi: 10.1089/ten.TEB.2016.0437pubmed: 27998240google scholar: lookup
              48. Lee JJ, Harrison JR, Boachie-Adjei K, Vargas E, Moley PJ. Platelet-Rich Plasma Injections With Needle Tenotomy for Gluteus Medius Tendinopathy: A Registry Study With Prospective Follow-up. Orthop J Sports Med 2016 Nov;4(11):2325967116671692.
                doi: 10.1177/2325967116671692pubmed: 27868077google scholar: lookup
              49. Zhou Y, Wang JH. PRP Treatment Efficacy for Tendinopathy: A Review of Basic Science Studies. Biomed Res Int 2016;2016:9103792.
                doi: 10.1155/2016/9103792pubmed: 27610386google scholar: lookup
              50. Geburek F, Gaus M, van Schie HT, Rohn K, Stadler PM. Effect of intralesional platelet-rich plasma (PRP) treatment on clinical and ultrasonographic parameters in equine naturally occurring superficial digital flexor tendinopathies - a randomized prospective controlled clinical trial. BMC Vet Res 2016 Sep 7;12(1):191.
                doi: 10.1186/s12917-016-0826-1pubmed: 27604193google scholar: lookup
              51. Zheng Z, Le H, Chen W, Shen W, Ouyang H. [Progress on treatment of tendinopathy with platelet-enriched plasma]. Zhejiang Da Xue Xue Bao Yi Xue Ban 2016 Mar;45(2):179-86.
                doi: 10.3785/j.issn.1008-9292.2016.03.11pubmed: 27273992google scholar: lookup
              52. Cheng P, Han P, Zhao C, Zhang S, Zhang X, Chai Y. Magnesium inference screw supports early graft incorporation with inhibition of graft degradation in anterior cruciate ligament reconstruction. Sci Rep 2016 May 23;6:26434.
                doi: 10.1038/srep26434pubmed: 27210585google scholar: lookup
              53. Wilson JJ, Lee KS, Chamberlain C, DeWall R, Baer GS, Greatens M, Kamps N. Intratendinous injections of platelet-rich plasma: feasibility and effect on tendon morphology and mechanics. J Exp Orthop 2015 Dec;2(1):5.
                doi: 10.1186/s40634-014-0018-5pubmed: 26914873google scholar: lookup
              54. Vahabi S, Vaziri S, Torshabi M, Rezaei Esfahrood Z. Effects of Plasma Rich in Growth Factors and Platelet-Rich Fibrin on Proliferation and Viability of Human Gingival Fibroblasts. J Dent (Tehran) 2015 Jul;12(7):504-12.
                pubmed: 26877740
              55. Cassano JM, Kennedy JG, Ross KA, Fraser EJ, Goodale MB, Fortier LA. Bone marrow concentrate and platelet-rich plasma differ in cell distribution and interleukin 1 receptor antagonist protein concentration. Knee Surg Sports Traumatol Arthrosc 2018 Jan;26(1):333-342.
                doi: 10.1007/s00167-016-3981-9pubmed: 26831858google scholar: lookup
              56. Chahla J, Dean CS, Moatshe G, Pascual-Garrido C, Serra Cruz R, LaPrade RF. Concentrated Bone Marrow Aspirate for the Treatment of Chondral Injuries and Osteoarthritis of the Knee: A Systematic Review of Outcomes. Orthop J Sports Med 2016 Jan;4(1):2325967115625481.
                doi: 10.1177/2325967115625481pubmed: 26798765google scholar: lookup
              57. González JC, López C, Álvarez ME, Pérez JE, Carmona JU. Autologous leukocyte-reduced platelet-rich plasma therapy for Achilles tendinopathy induced by collagenase in a rabbit model. Sci Rep 2016 Jan 19;6:19623.
                doi: 10.1038/srep19623pubmed: 26781753google scholar: lookup
              58. Sayegh ET, Sandy JD, Virk MS, Romeo AA, Wysocki RW, Galante JO, Trella KJ, Plaas A, Wang VM. Recent Scientific Advances Towards the Development of Tendon Healing Strategies. Curr Tissue Eng 2015;4(2):128-143.
                doi: 10.2174/2211542004666150713190231pubmed: 26753125google scholar: lookup
              59. Tiwari M, Bhargava R. Platelet rich plasma therapy: A comparative effective therapy with promising results in plantar fasciitis. J Clin Orthop Trauma 2013 Mar;4(1):31-5.
                doi: 10.1016/j.jcot.2013.01.008pubmed: 26403772google scholar: lookup
              60. Ekwueme EC, Shah JV, Mohiuddin M, Ghebes CA, Crispim JF, Saris DB, Fernandes HA, Freeman JW. Cross-Talk Between Human Tenocytes and Bone Marrow Stromal Cells Potentiates Extracellular Matrix Remodeling In Vitro. J Cell Biochem 2016 Mar;117(3):684-93.
                doi: 10.1002/jcb.25353pubmed: 26308651google scholar: lookup
              61. Zayni R, Thaunat M, Fayard JM, Hager JP, Carrillon Y, Clechet J, Gadea F, Archbold P, Sonnery Cottet B. Platelet-rich plasma as a treatment for chronic patellar tendinopathy: comparison of a single versus two consecutive injections. Muscles Ligaments Tendons J 2015 Apr-Jun;5(2):92-8.
                pubmed: 26261787
              62. Shin KH, Lee H, Kang S, Ko YJ, Lee SY, Park JH, Bae JH. Effect of Leukocyte-Rich and Platelet-Rich Plasma on Healing of a Horizontal Medial Meniscus Tear in a Rabbit Model. Biomed Res Int 2015;2015:179756.
                doi: 10.1155/2015/179756pubmed: 26180783google scholar: lookup
              63. Kisiday JD, McIlwraith CW, Rodkey WG, Frisbie DD, Steadman JR. Effects of Platelet-Rich Plasma Composition on Anabolic and Catabolic Activities in Equine Cartilage and Meniscal Explants. Cartilage 2012 Jul;3(3):245-54.
                doi: 10.1177/1947603511433181pubmed: 26069637google scholar: lookup
              64. von Wehren L, Blanke F, Todorov A, Heisterbach P, Sailer J, Majewski M. The effect of subacromial injections of autologous conditioned plasma versus cortisone for the treatment of symptomatic partial rotator cuff tears. Knee Surg Sports Traumatol Arthrosc 2016 Dec;24(12):3787-3792.
                doi: 10.1007/s00167-015-3651-3pubmed: 26017742google scholar: lookup
              65. Brossi PM, Moreira JJ, Machado TS, Baccarin RY. Platelet-rich plasma in orthopedic therapy: a comparative systematic review of clinical and experimental data in equine and human musculoskeletal lesions. BMC Vet Res 2015 Apr 22;11:98.
                doi: 10.1186/s12917-015-0403-zpubmed: 25896610google scholar: lookup
              66. Talaie T, Pratt SJP, Vanegas C, Xu S, Henn RF 3rd, Yarowsky P, Lovering RM. Site-specific targeting of platelet-rich plasma via superparamagnetic nanoparticles. Orthop J Sports Med 2015 Jan 22;3(1).
                doi: 10.1177/2325967114566185pubmed: 25664326google scholar: lookup
              67. Yanke AB, Chubinskaya S. The state of cartilage regeneration: current and future technologies. Curr Rev Musculoskelet Med 2015 Mar;8(1):1-8.
                doi: 10.1007/s12178-014-9254-7pubmed: 25591739google scholar: lookup
              68. Docheva D, Müller SA, Majewski M, Evans CH. Biologics for tendon repair. Adv Drug Deliv Rev 2015 Apr;84:222-39.
                doi: 10.1016/j.addr.2014.11.015pubmed: 25446135google scholar: lookup
              69. Sánchez M, Delgado D, Sánchez P, Fiz N, Azofra J, Orive G, Anitua E, Padilla S. Platelet rich plasma and knee surgery. Biomed Res Int 2014;2014:890630.
                doi: 10.1155/2014/890630pubmed: 25302310google scholar: lookup
              70. Drago L, Bortolin M, Vassena C, Romanò CL, Taschieri S, Del Fabbro M. Plasma components and platelet activation are essential for the antimicrobial properties of autologous platelet-rich plasma: an in vitro study. PLoS One 2014;9(9):e107813.
                doi: 10.1371/journal.pone.0107813pubmed: 25232963google scholar: lookup
              71. Giusti I, D'Ascenzo S, Mancò A, Di Stefano G, Di Francesco M, Rughetti A, Dal Mas A, Properzi G, Calvisi V, Dolo V. Platelet concentration in platelet-rich plasma affects tenocyte behavior in vitro. Biomed Res Int 2014;2014:630870.
                doi: 10.1155/2014/630870pubmed: 25147809google scholar: lookup
              72. Seijas R, Ares O, Cuscó X, Alvarez P, Steinbacher G, Cugat R. Partial anterior cruciate ligament tears treated with intraligamentary plasma rich in growth factors. World J Orthop 2014 Jul 18;5(3):373-8.
                doi: 10.5312/wjo.v5.i3.373pubmed: 25035842google scholar: lookup
              73. Factor D, Dale B. Current concepts of rotator cuff tendinopathy. Int J Sports Phys Ther 2014 Apr;9(2):274-88.
                pubmed: 24790788
              74. McCarrel TM, Mall NA, Lee AS, Cole BJ, Butty DC, Fortier LA. Considerations for the use of platelet-rich plasma in orthopedics. Sports Med 2014 Aug;44(8):1025-36.
                doi: 10.1007/s40279-014-0195-5pubmed: 24760591google scholar: lookup
              75. Kiapour AM, Murray MM. Basic science of anterior cruciate ligament injury and repair. Bone Joint Res 2014;3(2):20-31.
                doi: 10.1302/2046-3758.32.2000241pubmed: 24497504google scholar: lookup
              76. Pirvu TN, Schroeder JE, Peroglio M, Verrier S, Kaplan L, Richards RG, Alini M, Grad S. Platelet-rich plasma induces annulus fibrosus cell proliferation and matrix production. Eur Spine J 2014 Apr;23(4):745-53.
                doi: 10.1007/s00586-014-3198-xpubmed: 24469887google scholar: lookup
              77. Cheung EV, Silverio L, Yao J. Delivered growth factor therapy to improve healing after rotator cuff repair. Stem Cells Cloning 2010 Oct 11;3:135-44.
                doi: 10.2147/SCCAA.S7359pubmed: 24198519google scholar: lookup
              78. Smith RK, Werling NJ, Dakin SG, Alam R, Goodship AE, Dudhia J. Beneficial effects of autologous bone marrow-derived mesenchymal stem cells in naturally occurring tendinopathy. PLoS One 2013;8(9):e75697.
                doi: 10.1371/journal.pone.0075697pubmed: 24086616google scholar: lookup
              79. Halpern BC, Chaudhury S, Rodeo SA. The role of platelet-rich plasma in inducing musculoskeletal tissue healing. HSS J 2012 Jul;8(2):137-45.
                doi: 10.1007/s11420-011-9239-7pubmed: 23874254google scholar: lookup
              80. Borrione P, Grasso L, Chierto E, Geuna S, Racca S, Abbadessa G, Ronchi G, Faiola F, Di Gianfrancesco A, Pigozzi F. Experimental model for the study of the effects of platelet-rich plasma on the early phases of muscle healing. Blood Transfus 2014 Jan;12 Suppl 1(Suppl 1):s221-8.
                doi: 10.2450/2013.0275-12pubmed: 23867182google scholar: lookup
              81. Parafioriti A, Armiraglio E, Del Bianco S, Tibalt E, Oliva F, Berardi AC. Single injection of platelet-rich plasma in a rat Achilles tendon tear model. Muscles Ligaments Tendons J 2011 Apr;1(2):41-7.
                pubmed: 23738245
              82. Ferrero G, Fabbro E, Orlandi D, Martini C, Lacelli F, Serafini G, Silvestri E, Sconfienza LM. Ultrasound-guided injection of platelet-rich plasma in chronic Achilles and patellar tendinopathy. J Ultrasound 2012 Dec;15(4):260-6.
                doi: 10.1016/j.jus.2012.09.006pubmed: 23730392google scholar: lookup
              83. Fontenot RL, Sink CA, Werre SR, Weinstein NM, Dahlgren LA. Simple tube centrifugation for processing platelet-rich plasma in the horse. Can Vet J 2012 Dec;53(12):1266-72.
                pubmed: 23729823
              84. Middleton KK, Barro V, Muller B, Terada S, Fu FH. Evaluation of the effects of platelet-rich plasma (PRP) therapy involved in the healing of sports-related soft tissue injuries. Iowa Orthop J 2012;32:150-63.
                pubmed: 23576936
              85. Watson MG, Lopez JM, Paun M, Jones SA. A novel dynamic layer-by-layer assembled nano-scale biointerface: functionality tests with platelet adhesion and aggregate morphology influenced by adenosine diphosphate. J Thromb Thrombolysis 2013 Nov;36(4):448-57.
                doi: 10.1007/s11239-013-0905-0pubmed: 23456573google scholar: lookup
              86. Drago L, Bortolin M, Vassena C, Taschieri S, Del Fabbro M. Antimicrobial activity of pure platelet-rich plasma against microorganisms isolated from oral cavity. BMC Microbiol 2013 Feb 25;13:47.
                doi: 10.1186/1471-2180-13-47pubmed: 23442413google scholar: lookup
              87. Barbosa D, de Souza RA, de Carvalho WR, Xavier M, de Carvalho PK, Cunha TC, Arisawa EÂ, Silveira L Jr, Villaverde AB. Low-level laser therapy combined with platelet-rich plasma on the healing calcaneal tendon: a histological study in a rat model. Lasers Med Sci 2013 Nov;28(6):1489-94.
                doi: 10.1007/s10103-012-1241-xpubmed: 23307438google scholar: lookup
              88. Liang M, Cornell HR, Zargar Baboldashti N, Thompson MS, Carr AJ, Hulley PA. Regulation of hypoxia-induced cell death in human tenocytes. Adv Orthop 2012;2012:984950.
                doi: 10.1155/2012/984950pubmed: 23304533google scholar: lookup
              89. Campbell KJ, Boykin RE, Wijdicks CA, Erik Giphart J, LaPrade RF, Philippon MJ. Treatment of a hip capsular injury in a professional soccer player with platelet-rich plasma and bone marrow aspirate concentrate therapy. Knee Surg Sports Traumatol Arthrosc 2013 Jul;21(7):1684-8.
                doi: 10.1007/s00167-012-2232-ypubmed: 23052123google scholar: lookup
              90. Cole BJ, Seroyer ST, Filardo G, Bajaj S, Fortier LA. Platelet-rich plasma: where are we now and where are we going?. Sports Health 2010 May;2(3):203-10.
                doi: 10.1177/1941738110366385pubmed: 23015939google scholar: lookup
              91. Kaeding C, Best TM. Tendinosis: pathophysiology and nonoperative treatment. Sports Health 2009 Jul;1(4):284-92.
                doi: 10.1177/1941738109337778pubmed: 23015885google scholar: lookup
              92. Spaas JH, Guest DJ, Van de Walle GR. Tendon regeneration in human and equine athletes: Ubi Sumus-Quo Vadimus (where are we and where are we going to)?. Sports Med 2012 Oct 1;42(10):871-90.
                doi: 10.1007/BF03262300pubmed: 22963225google scholar: lookup
              93. Dhillon RS, Schwarz EM, Maloney MD. Platelet-rich plasma therapy - future or trend?. Arthritis Res Ther 2012 Aug 8;14(4):219.
                doi: 10.1186/ar3914pubmed: 22894643google scholar: lookup
              94. Fortier LA, Barker JU, Strauss EJ, McCarrel TM, Cole BJ. The role of growth factors in cartilage repair. Clin Orthop Relat Res 2011 Oct;469(10):2706-15.
                doi: 10.1007/s11999-011-1857-3pubmed: 21403984google scholar: lookup
              95. Fortier LA, Travis AJ. Stem cells in veterinary medicine. Stem Cell Res Ther 2011 Feb 23;2(1):9.
                doi: 10.1186/scrt50pubmed: 21371354google scholar: lookup
              96. Watts AE, Yeager AE, Kopyov OV, Nixon AJ. Fetal derived embryonic-like stem cells improve healing in a large animal flexor tendonitis model. Stem Cell Res Ther 2011 Jan 27;2(1):4.
                doi: 10.1186/scrt45pubmed: 21272343google scholar: lookup
              97. Kon E, Filardo G, Di Martino A, Marcacci M. Platelet-rich plasma (PRP) to treat sports injuries: evidence to support its use. Knee Surg Sports Traumatol Arthrosc 2011 Apr;19(4):516-27.
                doi: 10.1007/s00167-010-1306-ypubmed: 21082164google scholar: lookup
              98. Georg R, Maria C, Gisela A, Bianca C. Autologous conditioned plasma as therapy of tendon and ligament lesions in seven horses. J Vet Sci 2010 Jun;11(2):173-5.
                doi: 10.4142/jvs.2010.11.2.173pubmed: 20458161google scholar: lookup
              99. Nazaroglou I, Stavrianos C, Kafas P, Matoulas E, Upile T, Barlas I, Jerjes W. Radiographic evaluation of bone regeneration after the application of plasma rich in growth factors in a lower third molar socket: a case report. Cases J 2009 Dec 3;2:9134.
                doi: 10.1186/1757-1626-2-9134pubmed: 20062651google scholar: lookup
              100. Murray SJ, Santangelo KS, Bertone AL. Evaluation of early cellular influences of bone morphogenetic proteins 12 and 2 on equine superficial digital flexor tenocytes and bone marrow-derived mesenchymal stem cells in vitro. Am J Vet Res 2010 Jan;71(1):103-14.
                doi: 10.2460/ajvr.71.1.103pubmed: 20043789google scholar: lookup
              101. Koch TG, Berg LC, Betts DH. Current and future regenerative medicine - principles, concepts, and therapeutic use of stem cell therapy and tissue engineering in equine medicine. Can Vet J 2009 Feb;50(2):155-65.
                pubmed: 19412395
              102. Hammond JW, Hinton RY, Curl LA, Muriel JM, Lovering RM. Use of autologous platelet-rich plasma to treat muscle strain injuries. Am J Sports Med 2009 Jun;37(6):1135-42.
                doi: 10.1177/0363546508330974pubmed: 19282509google scholar: lookup
              103. Mishra A, Tummala P, King A, Lee B, Kraus M, Tse V, Jacobs CR. Buffered platelet-rich plasma enhances mesenchymal stem cell proliferation and chondrogenic differentiation. Tissue Eng Part C Methods 2009 Sep;15(3):431-5.
                doi: 10.1089/ten.tec.2008.0534pubmed: 19216642google scholar: lookup
              104. Rabago D, Best TM, Zgierska AE, Zeisig E, Ryan M, Crane D. A systematic review of four injection therapies for lateral epicondylosis: prolotherapy, polidocanol, whole blood and platelet-rich plasma. Br J Sports Med 2009 Jul;43(7):471-81.
                doi: 10.1136/bjsm.2008.052761pubmed: 19028733google scholar: lookup
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