Point mutation of neu oncogene in animal peripheral nerve sheath tumors.
Abstract: Thirty-four peripheral nerve sheath tumors of four domesticated animal species were characterized and assayed for point mutation of the neu oncogene. Based on their morphoimmunophenotype, 32 tumors were classified as schwannomas. Schwannoma morphology was characterized by the presence of Antoni type A and B pattern and immunoreactivity for S-100 protein and vimentin. Two anaplastic and metastatic tumors originating from spinal cord root, immunonegative for S-100 protein and positive for vimentin, were classified as malignant peripheral nerve sheath tumors (MPNSTs). Four malignant schwannomas and two MPNSTs expressed a point mutation of the neu oncogene by the polymerase chain reaction-restriction fragment length polymorphism method. The finding of neu oncogene mutation could be a useful diagnostic genetic marker in the malignant form of peripheral nerve sheath tumors in animals.
Publication Date: 2001-12-06 PubMed ID: 11732802DOI: 10.1354/vp.38-6-679Google Scholar: Lookup
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- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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This research investigated a specific genetic mutation, known as the neu oncogene, in 34 peripheral nerve sheath tumors in animals. The study found that this mutation was present in some malignant forms of these tumors, which suggests it could be used as a diagnostic genetic marker.
Analysis of Peripheral Nerve Sheath Tumors in Animals
- The study examined 34 peripheral nerve sheath tumors from four different domesticated animal species.
- The peripheral nerve sheath tumors were characterized and tested for point mutation of the neu oncogene. Point mutations are a type of mutation that involves the replacement of a single base nucleotide with another nucleotide. These can sometimes lead to drastic changes in protein function, such as creating an oncogenic (cancer-causing) protein.
- Based on their morphoimmunophenotype, which encompasses the physical characteristics and the immune response of the tumors, 32 of the tumors were classified as schwannomas.
- Schwannomas are tumors that originate from Schwann cells, which are the cells responsible for producing the myelin sheath covering nerve fibers. These tumors are characterized by a specific pattern known as Antoni type A and B, and an immune response for S-100 protein and vimentin.
Identification of Malignant Peripheral Nerve Sheath Tumors (MPNSTs)
- Two anaplastic and metastatic tumors (those which can invade other tissues and spread to other parts of the body) that originated from the spinal cord root were classified as Malignant Peripheral Nerve Sheath Tumors (MPNSTs).
- These MPNSTs were immunonegative for S-100 protein but tested positive for vimentin, highlighting the differences between the benign schwannomas and the malignant MPNSTs.
Presence of neu Oncogene Mutation in Malignant Tumors
- Out of all the analyzed tumors, four malignant schwannomas and the two MPNSTs showed a point mutation in the neu oncogene. This was determined using the polymerase chain reaction-restriction fragment length polymorphism method, a technique used to detect specific mutations in DNA.
- This finding is significant as it establishes a possible link between the neu oncogene point mutation and the malignant forms of peripheral nerve sheath tumors. This indicates that the presence of the neu oncogene mutation can potentially be used as a diagnostic genetic marker in identifying malignant peripheral nerve sheath tumors in animals.
Cite This Article
APA
Stoica G, Tasca SI, Kim HT.
(2001).
Point mutation of neu oncogene in animal peripheral nerve sheath tumors.
Vet Pathol, 38(6), 679-688.
https://doi.org/10.1354/vp.38-6-679 Publication
Researcher Affiliations
- Texas A&M University, Department of Veterinary Pathobiology, College Station 77843, USA. gstoica@cvm.tamu.ed
MeSH Terms
- Animal Diseases / genetics
- Animal Diseases / pathology
- Animals
- Cat Diseases / genetics
- Cat Diseases / pathology
- Cats
- Cattle
- Cattle Diseases / genetics
- Cattle Diseases / pathology
- DNA, Neoplasm / genetics
- Dog Diseases / genetics
- Dog Diseases / pathology
- Dogs
- Female
- Genes, erbB-2 / genetics
- Horse Diseases / genetics
- Horse Diseases / pathology
- Horses
- Immunohistochemistry / veterinary
- Male
- Nerve Sheath Neoplasms / genetics
- Nerve Sheath Neoplasms / pathology
- Nerve Sheath Neoplasms / veterinary
- Neurilemmoma / genetics
- Neurilemmoma / pathology
- Neurilemmoma / veterinary
- Point Mutation
- Polymerase Chain Reaction / veterinary
- Retrospective Studies
- Sequence Analysis, DNA
Citations
This article has been cited 10 times.- Boland L, Setyo L, Sangster C, Brunel L, Foo T, Bennett P. Colonic malignant peripheral nerve sheath tumour in a cat. JFMS Open Rep 2019 Jan-Jun;5(1):2055116919849979.
- Poli F, Calistri M, Mandara MT, Baroni M. Central nervous system metastasis of an intradural malignant peripheral nerve sheath tumor in a dog. Open Vet J 2019 Apr;9(1):49-53.
- Ribas A, Miller R, Rasotto R. Small intestinal peripheral nerve sheath tumour in a cat. JFMS Open Rep 2017 Jan-Jun;3(1):2055116917710394.
- Resende TP, Pereira CE, Vannucci FA, Araujo FS, dos Santos JL, Cassali GD, Damasceno KA, Guedes RM. Malignant peripheral nerve sheath tumour in a sow. Acta Vet Scand 2015 Sep 25;57:56.
- Sayama A, Okado K, Imaoka M, Yokouchi Y, Jindo T, Takasaki W. Subcutaneous soft tissue sarcoma with rhabdoid features in a dog. J Toxicol Pathol 2014 Jul;27(2):131-8.
- Holtkamp N, Malzer E, Zietsch J, Okuducu AF, Mucha J, Mawrin C, Mautner VF, Schildhaus HU, von Deimling A. EGFR and erbB2 in malignant peripheral nerve sheath tumors and implications for targeted therapy. Neuro Oncol 2008 Dec;10(6):946-57.
- Saito R, Chambers JK, Uchida K. Comparative study of feline spinal cord tumors for distinction between peripheral nerve sheath tumor and meningioma. J Vet Med Sci 2025 Jul 1;87(7):747-751.
- Bochynska D, Sharpe A, Toland B, Demeter EA. Schwannosis in the brain of a neonatal calf. J Vet Diagn Invest 2025 Jan;37(1):160-163.
- Cooper-Khan RS, Frankovich AN, Thompson CA, Thomovsky SA, Lewis MJ. Clinical Findings and Outcome in 30 Dogs with Presumptive or Confirmed Nerve Sheath Tumors. Vet Sci 2024 Apr 28;11(5).
- Patchett AL, Flies AS, Lyons AB, Woods GM. Curse of the devil: molecular insights into the emergence of transmissible cancers in the Tasmanian devil (Sarcophilus harrisii). Cell Mol Life Sci 2020 Jul;77(13):2507-2525.
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