Polymerase chain reaction analysis of the surgical margins of equine sarcoids for bovine papilloma virus DNA.
Abstract: To examine apparently normal skin around equine sarcoids for evidence of bovine papilloma virus (BPV) DNA, and to relate this finding to the observed recurrence after surgery. Methods: Prospective study. Methods: Forty-one equine sarcoids from 19 horses. Methods: The tumors were surgically excised at a measured distance of 8, 12, or 16 mm. Samples from the tumor and of the entire surrounding skin were taken at 4, 8, 12, and 16 mm from the tumor border and analyzed for the presence of BPV DNA using polymerase chain reaction (PCR) amplification. The samples were grouped per examined sarcoid, and a tumor was considered positive at a certain distance as soon as at least one of the samples at that distance was positive. The clinical outcome was recorded for each sarcoid after a minimal follow-up of 6 months. Results: All sarcoids were positive for BPV(1) or BPV(2). The tumor margin was positive at 4, 8, 12, and 16 mm in, respectively, 95%, 73%, 39%, and 33% of the examined sarcoids. Local recurrence was observed in 3 sarcoids on 3 different horses. From survival analysis, there was a greater likelihood for local recurrence when sarcoids had a surgical margin that was positive for BPV DNA. Conclusions: BPV DNA is often detected in visibly normal skin around sarcoids, and there is a significantly greater probability for local recurrence when the surgical margins are positive for the presence of BPV DNA.
Copyright 2001 by The American College of Veterinary Surgeons
Publication Date: 2001-09-14 PubMed ID: 11555822DOI: 10.1053/jvet.2001.25874Google Scholar: Lookup
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- Journal Article
Summary
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The study investigates the presence of bovine papilloma virus (BPV) DNA in the surrounding normal skin of equine sarcoids in horses, its correlation to surgical margins, and its potential impact on relapse after surgery.
Methods
- 41 equine sarcoids from 19 different horses were examined in this prospective study. The sarcoids were surgically excised at intervals of 8, 12, or 16 mm.
- Samples from the tumor and the entire surrounding skin were taken at 4, 8, 12, and 16 mm from the tumor border. These were analyzed for the presence of BPV DNA using polymerase chain reaction (PCR) amplification.
- Samples were grouped together for each sarcoid. A sarcoid was considered positive if any of the samples at a particular distance came out positive in the analysis.
- The clinical outcomes for each sarcoid were recorded after a minimum follow-up period of six months.
Results
- All examined sarcoids were positive for BPV1 or BPV2.
- The tumour border, when analyzed at 4, 8, 12, and 16 mm distances, was positive in 95%, 73%, 39%, and 33% of the sarcoids, respectively.
- Of the 19 horses, local recurrence was observed in three sarcoids on three different horses.
- Through survival analysis, a higher likelihood of recurrence was found in sarcoids that had surgical margins positive for BPV DNA.
Conclusions
- Results showed that BPV DNA is often present in the visibly healthy skin around equine sarcoids. The margins around the surgically treated area also yielded a high presence of BPV DNA.
- This study suggests a significant correlation between the presence of BPV DNA in surgical margins and a higher risk of recurrence. As such, the results could influence future surgical decisions regarding wider removal margins to inhibit recurrence.
Cite This Article
APA
Martens A, De Moor A, Demeulemeester J, Peelman L.
(2001).
Polymerase chain reaction analysis of the surgical margins of equine sarcoids for bovine papilloma virus DNA.
Vet Surg, 30(5), 460-467.
https://doi.org/10.1053/jvet.2001.25874 Publication
Researcher Affiliations
- Department of Large Animal Surgery and Anesthesiology, Faculty of Veterinary Medicine, Ghent University, Belgium.
MeSH Terms
- Animals
- Bovine papillomavirus 1 / genetics
- Bovine papillomavirus 1 / isolation & purification
- DNA, Viral / genetics
- Disease-Free Survival
- Electrophoresis, Agar Gel / veterinary
- Female
- Horse Diseases / pathology
- Horse Diseases / surgery
- Horse Diseases / virology
- Horses
- Male
- Neoplasm Recurrence, Local / veterinary
- Neoplasm Recurrence, Local / virology
- Papillomavirus Infections / veterinary
- Papillomavirus Infections / virology
- Polymerase Chain Reaction / veterinary
- Prospective Studies
- Sarcoidosis / veterinary
- Sarcoidosis / virology
- Skin Neoplasms / pathology
- Skin Neoplasms / surgery
- Skin Neoplasms / veterinary
- Treatment Outcome
- Tumor Virus Infections / veterinary
- Tumor Virus Infections / virology
Citations
This article has been cited 11 times.- Saba C, Eggleston R, Parks A, Peroni J, Sjoberg E, Rice S, Tyma J, Williams J, Grosenbaugh D, Leard AT. ALVAC-fIL2, a feline interleukin-2 immunomodulator, as a treatment for sarcoids in horses: A pilot study. J Vet Intern Med 2022 May;36(3):1179-1184.
- Savini F, Gallina L, Mazza F, Mariella J, Castagnetti C, Scagliarini A. Molecular Detection of Bovine Papillomavirus DNA in the Placenta and Blood of Healthy Mares and Respective Foals. Vet Sci 2019 Feb 6;6(1).
- Martano M, Corteggio A, Restucci B, De Biase ME, Borzacchiello G, Maiolino P. Extracellular matrix remodeling in equine sarcoid: an immunohistochemical and molecular study. BMC Vet Res 2016 Feb 2;12:24.
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- Finlay M, Yuan Z, Morgan IM, Campo MS, Nasir L. Equine sarcoids: Bovine Papillomavirus type 1 transformed fibroblasts are sensitive to cisplatin and UVB induced apoptosis and show aberrant expression of p53. Vet Res 2012 Dec 4;43(1):81.
- Wobeser BK, Davies JL, Hill JE, Jackson ML, Kidney BA, Mayer MN, Townsend HG, Allen AL. Epidemiology of equine sarcoids in horses in western Canada. Can Vet J 2010 Oct;51(10):1103-8.
- Antonsson A, Hansson BG. Healthy skin of many animal species harbors papillomaviruses which are closely related to their human counterparts. J Virol 2002 Dec;76(24):12537-42.
- Vychodilova L, Plasil M, Futas J, Kopecka A, Molinkova D, Wijacki T, Jahn P, Knoll A, Horin P. Genetic susceptibility to sarcoid in Arabian horses: associations with MHC class II and compound MHC class I/KLRA genotypes. Vet Res Commun 2025 May 1;49(3):184.
- Sobhy NM, Refaai W, Kumar R, Bottros Youssef CR, Goyal SM. Molecular Characterization of Bovine Deltapapillomavirus in Equine Sarcoids in Egypt. Vet Med Int 2025;2025:9773642.
- Paietta EN, Kraberger S, Regney M, Custer JM, Ehmke E, Yoder AD, Varsani A. Interspecies Papillomavirus Type Infection and a Novel Papillomavirus Type in Red Ruffed Lemurs (Varecia rubra). Viruses 2023 Dec 25;16(1).
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