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Home / Equine Research Bank / Anim Reprod Sci / Potential involvement of EGF-like growth factors and phospho...
Animal reproduction science2007; 103(1-2); 187-192; doi: 10.1016/j.anireprosci.2007.04.006

Potential involvement of EGF-like growth factors and phosphodiesterases in initiation of equine oocyte maturation.

Abstract: Human chorionic gonadotropin (hCG) was administered to mares in estrus with large, dominant ovarian follicles to initiate follicular and oocyte maturation. Follicular contents were collected at 0, 2, 4 and 6 h after hCG. Epiregulin, amphiregulin and phosphodiesterase (PDE) mRNA contents of granulosa cells (PDE 4D) were determined by reverse transcription and real-time PCR; PDE 3A mRNA content of single oocytes was determined similarly. Copy numbers of mRNA did not increase for PDE 3A or 4D over the time interval studied. Amounts of epiregulin and amphiregulin mRNA were correlated (r=0.98) when log transformed. Epiregulin and amphiregulin mRNA increased (P<0.01) from controls by 4 h after hCG administration, with amphiregulin increasing (P<0.01) by 2 h after hCG administration. Epiregulin and amphiregulin mRNA levels remained elevated (P<0.01) at 6h after hCG. These results indicate that EGF-like growth factors are likely paracrine mediators of the LH signal in the horse.
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Publication Date: 2007-04-20 PubMed ID: 17507186DOI: 10.1016/j.anireprosci.2007.04.006Google Scholar: Lookup
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  • Journal Article
  • Research Support
  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research explores the potential role of EGF-like growth factors and phosphodiesterases in initiating the maturation of equine oocytes. The overall increase in EGF levels and their correlation suggest they could be mediating the LH signal in the horse.

Research Methodology

  • The researchers administered Human chorionic gonadotropin (hCG), a hormone triggering ovulation, to mares in estrus.
  • These mares had large, dominant ovarian follicles—an ideal state for oocyte (egg cell) maturation.
  • The content of follicles was collected at different intervals of time (0, 2, 4 and 6 hours) after the hCG administration.

Experimental Analysis

  • The concentrations of Epiregulin, Amphiregulin and Phosphodiesterase (PDE) mRNA in the granulosa cells (cells surrounding the egg cell in an ovary) were determined using reverse transcription and real-time PCR techniques.
  • The content of PDE 3A mRNA in single oocytes was likewise determined.
  • However, there was no increase observed in the copy numbers of either PDE 3A or PDE 4D mRNA over the time interval studied.

Role of EGF-like Growth Factors

  • The study found a strong correlation (r=0.98) between the amounts of Epiregulin and Amphiregulin mRNA when log transformed.
  • Epiregulin and Amphiregulin mRNA also showed an increase from the control groups by 4 hours after hCG administration, with Amphiregulin increasing even earlier, by 2 hours.
  • The mRNA levels remained high even at 6 hours after the hCG administration.

Significance of the Results

  • The results of the study suggest a probable role of Epiregulin and Amphiregulin, both EGF-like growth factors, as the paracrine mediators of the luteinizing hormone (LH) signal in the horse.
  • The consistent increase in their levels post hCG administration and their correlation emphasizes their potential involvement in initiating oocyte maturation in equines.

Cite This Article

APA
Lindbloom SM, Farmerie TA, Clay CM, Seidel GE, Carnevale EM. (2007). Potential involvement of EGF-like growth factors and phosphodiesterases in initiation of equine oocyte maturation. Anim Reprod Sci, 103(1-2), 187-192. https://doi.org/10.1016/j.anireprosci.2007.04.006

Publication

Animal reproduction science
ISSN: 0378-4320
NlmUniqueID: 7807205
Country: Netherlands
Language: English
Volume: 103
Issue: 1-2
Pages: 187-192

Researcher Affiliations

Lindbloom, S M
  • Animal Reproduction and Biotechnology Laboratory, Colorado State University, Fort Collins, Colorado 80523-1683, USA.
Farmerie, T A
    Clay, C M
      Seidel, G E
        Carnevale, E M

          MeSH Terms

          • Amphiregulin
          • Animals
          • Base Sequence
          • Chorionic Gonadotropin / pharmacology
          • EGF Family of Proteins
          • Epidermal Growth Factor / biosynthesis
          • Epidermal Growth Factor / genetics
          • Epiregulin
          • Female
          • Glycoproteins / biosynthesis
          • Glycoproteins / genetics
          • Horses / metabolism
          • Horses / physiology
          • Intercellular Signaling Peptides and Proteins / biosynthesis
          • Intercellular Signaling Peptides and Proteins / genetics
          • Molecular Sequence Data
          • Oocytes / enzymology
          • Oocytes / metabolism
          • Ovarian Follicle / growth & development
          • Ovarian Follicle / metabolism
          • Phosphoric Diester Hydrolases / biosynthesis
          • Phosphoric Diester Hydrolases / genetics
          • RNA, Messenger / biosynthesis
          • RNA, Messenger / genetics
          • Random Allocation
          • Reverse Transcriptase Polymerase Chain Reaction / veterinary

          Citations

          This article has been cited 8 times.
          1. Ahmed AE, Sindi RA, Yousef NA, Hussein HA, Badr MR, Syaad KMA, Al-Saeed FA, Hassaneen ASA, Abdelrahman M, Ali ME. Impact of epidermal growth factor and/or β-mercaptoethanol supplementations on the in vitro produced buffaloes' embryos. Front Vet Sci 2023;10:1138220.
            doi: 10.3389/fvets.2023.1138220pubmed: 36992972google scholar: lookup
          2. Hale BJ, Li Y, Adur MK, Ross JW. Inhibition of germinal vesicle breakdown using IBMX increases microRNA-21 in the porcine oocyte. Reprod Biol Endocrinol 2020 May 11;18(1):39.
            doi: 10.1186/s12958-020-00603-1pubmed: 32393269google scholar: lookup
          3. Chang ZL, Li BX, Liu B, Yao L, Yu J, Jiang GM, Tan JH. Effects of FSH and the weather during induced ovulation and timed artificial insemination to increase jenny conception rates. Sci Rep 2019 Mar 1;9(1):3220.
            doi: 10.1038/s41598-019-39757-3pubmed: 30824770google scholar: lookup
          4. Yong H, Oh HI, Lee SH, Cheong HT, Yang BK, Park CK. Treatment of Epidermal Growth Factor (EGF) enhances Nuclear Maturation of Porcine Oocytes and Stimulates Expression of ER/Golgi Transport Proteins. Dev Reprod 2017 Jun;21(2):131-138.
            doi: 10.12717/DR.2017.21.2.131pubmed: 28785734google scholar: lookup
          5. Zhang K, Smith GW. Maternal control of early embryogenesis in mammals. Reprod Fertil Dev 2015 Jul;27(6):880-96.
            doi: 10.1071/RD14441pubmed: 25695370google scholar: lookup
          6. Farsi MM, Kamali N, Pourghasem M. Embryological aspects of oocyte in vitro maturation. Int J Mol Cell Med 2013 Summer;2(3):99-109.
            pubmed: 24551799
          7. Conti M, Hsieh M, Zamah AM, Oh JS. Novel signaling mechanisms in the ovary during oocyte maturation and ovulation. Mol Cell Endocrinol 2012 Jun 5;356(1-2):65-73.
            doi: 10.1016/j.mce.2011.11.002pubmed: 22101318google scholar: lookup
          8. Hsieh M, Zamah AM, Conti M. Epidermal growth factor-like growth factors in the follicular fluid: role in oocyte development and maturation. Semin Reprod Med 2009 Jan;27(1):52-61.
            doi: 10.1055/s-0028-1108010pubmed: 19197805google scholar: lookup
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