Precision and accuracy of a point-of-care glucometer in horses and the effects of sample type.
Abstract: Point-of-care glucometry is used commonly in clinical and research settings; however, accuracy and precision of this method are concerns. The objectives of this study were to determine the accuracy of glucometry in adult horses and the precision of duplicate measurements. Blood samples were collected from 62 horses into one plain syringe, one EDTA tube and three fluoride oxalate (FO) tubes. Immediately after collection, glucose concentrations in whole blood were determined, in duplicate, by glucometry from the syringe (plain whole blood [WB] group), EDTA tube (EDTA group) and one FO tube (FO group). One FO sample was used to measure plasma glucose concentration by a laboratory chemistry analyser (LAB group) ≤1 h after collection. The third FO tube was used to measure plasma glucose concentration by glucometry after 3 h storage (FO3hr group). Adequate precision was present for all groups (coefficient of variation: 0.7-3.5%) except WB (5.5-9.4%). Between groups, correlations were significant (P < 0.05; except for WB-EDTA), varied with group comparison, and tended to be lowest for comparisons involving WB. Mean bias was lowest for WB-LAB and greatest for FO-LAB and FO3hr-LAB; however, the limits of agreement were ≥4.65 mmol/L for WB-LAB and ≤2.75 mmol/L for most other comparisons. For the glucometer used, performance was influenced by sample type: WB was unsuitable, while FO or EDTA samples resulted in adequate precision and accuracy, provided under-estimation of glucose concentrations is accounted for by using method-specific reference ranges. Glucometer performance and optimal sample type(s) should be determined prior to use in horses.
Crown Copyright © 2019. Published by Elsevier Ltd. All rights reserved.
Publication Date: 2019-08-23 PubMed ID: 31554585DOI: 10.1016/j.tvjl.2019.105359Google Scholar: Lookup
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- Evaluation Study
- Journal Article
- Adult Horses
- Analytical Methods
- Blood Analysis
- Clinical Findings
- Clinical Pathology
- Clinical Study
- Comparative Study
- Diagnosis
- Diagnostic Technique
- Disease Diagnosis
- Equine Diseases
- Equine Health
- Glucose
- Horses
- Laboratory Methods
- Physiology
- Veterinary Medicine
- Veterinary Practice
- Veterinary Research
- Veterinary Science
Summary
This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.
The research studied the precision and accuracy of a glucometer used at point-of-care on horses. The study revealed that the glucometer performance was influenced by the type of blood sample used and suggested optimal sample types should be determined before use on horses.
Objective and Methodology of the Study
- The objective of the study carried out was to evaluate the accuracy and precision of glucometers when used on adult horses, as well as the precision of duplicate measurements.
- Blood samples were obtained from 62 horses using one plain syringe, one Ethylenediaminetetraacetic acid (EDTA) tube, and three fluoride oxalate (FO) tubes.
- The glucose concentration in whole blood was determined immediately after collection using a glucometer from the syringe, EDTA, and one FO tube.
Test Groups in the Study
- The study categorized the blood samples into five groups based on the collection method. These groups included the plain whole blood group (WB), the EDTA group, one FO tube group (FO), one lab group (LAB), and one group used to measure glucose concentration after three hours storage (FO3hr).
- In the LAB group, plasma glucose concentration was measured using a laboratory chemistry analyzer within one hour of collection.
- In the FO3hr group, plasma glucose concentration was measured by glucometry after a storage period of 3 hours.
Findings of the Study
- The study found that there was adequate precision in all groups, excluding the WB group, where the coefficient of variation was 5.5-9.4%.
- Correlations were significant between groups, barring WB-EDTA group, however, there were group-based variations and they were lowest for comparisons involving WB group.
- The least mean bias was found in the WB-LAB group and the highest was found in FO-LAB and FO3hr-LAB groups.
Conclusion of the Study
- The study concluded that the performance of the used glucometer is influenced by the type of sample used. The whole blood sample was found to be unsuitable, while Fluoride Oxalate or EDTA samples resulted in adequate precision and accuracy only when glucose concentrations were under-estimated using method-specific reference ranges.
- It suggested that the performance of the glucometer and the optimal sample type should be determined prior to its use in horses.
Cite This Article
APA
Rendle DI, Armstrong SK, Heller J, Hughes KJ.
(2019).
Precision and accuracy of a point-of-care glucometer in horses and the effects of sample type.
Vet J, 252, 105359.
https://doi.org/10.1016/j.tvjl.2019.105359 Publication
Researcher Affiliations
- School of Animal and Veterinary Sciences, Charles Sturt University, Locked Bag 588, Boorooma Street, Wagga Wagga, NSW, 2678, Australia; Rainbow Equine Hospital, Old Malton, Malton, North Yorkshire, YO17 6SG, UK.
- School of Animal and Veterinary Sciences, Charles Sturt University, Locked Bag 588, Boorooma Street, Wagga Wagga, NSW, 2678, Australia; Royal (Dick) School of Veterinary Studies and Roslin Institute, University of Edinburgh, Roslin, Midlothian, EH25 9RG, UK.
- School of Animal and Veterinary Sciences, Charles Sturt University, Locked Bag 588, Boorooma Street, Wagga Wagga, NSW, 2678, Australia.
- School of Animal and Veterinary Sciences, Charles Sturt University, Locked Bag 588, Boorooma Street, Wagga Wagga, NSW, 2678, Australia. Electronic address: krhughes@csu.edu.au.
MeSH Terms
- Animals
- Blood Glucose / metabolism
- Female
- Horse Diseases / blood
- Horse Diseases / diagnosis
- Horses / blood
- Hyperglycemia / diagnosis
- Hyperglycemia / veterinary
- Male
- Point-of-Care Systems
- Reference Values
- Reproducibility of Results
Citations
This article has been cited 1 times.- Wong D, Malik C, Dembek K, Estell K, Marchitello M, Wilson K. Evaluation of a continuous glucose monitoring system in neonatal foals.. J Vet Intern Med 2021 Jul;35(4):1995-2001.
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