Predominance of G3B and G14 equine group A rotaviruses of a single VP4 serotype in Japan.
Abstract: A total of 65 equine group A rotaviruses (GAR) isolated from diarrheal foals at 48 farms in Hokkaido, Japan, between 1996 (29 isolates) and 1997 (36 isolates) were characterized for their VP7 and VP4 serotypes by PCR, nucleotide sequencing, and virus neutralization (VN) tests. By PCR VP7 typing, all isolates were classified as G3 or G 14, and the predominant serotype in each year was G3 (86%) in 1996 and G14 (53%) in 1997. VN tests with these 20 isolates randomly selected confirmed the specificity of PCR on the bases of complete agreement of the results in these methods (9 G3 and 11 G14), and revealed that all 9 G3 isolates were subtype G3B. There were five differing amino acid residues in three VP7 antigenic regions between subtypes G3A and G3B. Antiserum to a baculovirus recombinant that expressed P[12] VP4 neutralized all isolates and P[12] reference strains. These results suggest that genotype P[12] GAR belong to a single VP4 serotype, and that one VP4 and two VP7 serotypes (G3B and G14) of GAR were predominant in the equine population in Japan.
Publication Date: 2001-11-28 PubMed ID: 11722016PubMed Central: PMC7087255DOI: 10.1007/s007050170044Google Scholar: Lookup
The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.
This article describes a study revolving around the identification of the predominant strains of equine group A rotaviruses (GAR) in Japan, during 1996 and 1997, using various virology techniques such as PCR, nucleotide sequencing and virus neutralization tests.
Objective of the Research
- The primary aim of this research was to identify and characterize the predominant strains of equine group A rotaviruses (GAR) that were isolated from diarrheal foals in Hokkaido, Japan, during the years 1996 and 1997.
Methodology and Findings
- The research team collected a total of 65 GAR tractions from diarrhea-affected foals at 48 different farms between the years 1996 (29 samples) and 1997 (36 samples).
- All these isolates were then subjected to an analysis for their VP7 and VP4 serotypes via a series of processes— PCR, nucleotide sequencing, and virus neutralization (VN) tests.
- The PCR VP7 typing classified all of the isolated samples as either G3 or G14. In the year 1996, the dominant serotype was G3 (86%). Conversely, the following year saw a shift in this dominance to serotype G14 (53%).
- The researchers then selected 20 isolates at random to confirm the specificity of the PCR outcome using VN tests. The testing revealed a total agreement of results between these methods. Also, the tests identified that all nine G3 isolates were subtype G3B.
- Furthermore, the observation of the three VP7 antigenic regions showed that there was a variation in five amino acid residues between the subtypes G3A and G3B.
Conclusions
- Antisera produced against a recombinant baculovirus that expressed P[12] VP4 were successful in neutralizing all the isolates and P[12] reference strains. This result provides substantial evidence to suggest that all genotype P[12] GARs belong to a single VP4 serotype.
- The study concluded that in the equine population of Hokkaido, Japan, one VP4 and two VP7 serotypes (G3B and G14) of group A rotaviruses are predominant.
Cite This Article
APA
Tsunemitsu H, Imagawa H, Togo M, Shouji T, Kawashima K, Horino R, Imai K, Nishimori T, Takagi M, Higuchi T.
(2001).
Predominance of G3B and G14 equine group A rotaviruses of a single VP4 serotype in Japan.
Arch Virol, 146(10), 1949-1962.
https://doi.org/10.1007/s007050170044 Publication
Researcher Affiliations
- Shichinohe Research Unit, National Institute of Animal Health, Aomori, Japan. tsunemi@affrc.go.jp
MeSH Terms
- Amino Acid Sequence
- Animals
- Antigens, Viral
- Capsid / chemistry
- Capsid / genetics
- Capsid / immunology
- Capsid Proteins
- Horses / virology
- Humans
- Immune Sera / immunology
- Molecular Sequence Data
- Polymerase Chain Reaction
- Rotavirus / classification
- Serotyping
Citations
This article has been cited 10 times.- Uprety T, Sreenivasan CC, Hause BM, Li G, Odemuyiwa SO, Locke S, Morgan J, Zeng L, Gilsenan WF, Slovis N, Metcalfe L, Carter CN, Timoney P, Horohov D, Wang D, Erol E, Adam E, Li F. Identification of a Ruminant Origin Group B Rotavirus Associated with Diarrhea Outbreaks in Foals. Viruses 2021 Jul 9;13(7).
- Nemoto M, Matsumura T. Equine rotavirus infection. J Equine Sci 2021 Mar;32(1):1-9.
- Carossino M, Barrandeguy ME, Erol E, Li Y, Balasuriya UBR. Development and evaluation of a one-step multiplex real-time TaqMan(®) RT-qPCR assay for the detection and genotyping of equine G3 and G14 rotaviruses in fecal samples. Virol J 2019 Apr 25;16(1):49.
- Bailey KE, Gilkerson JR, Browning GF. Equine rotaviruses--current understanding and continuing challenges. Vet Microbiol 2013 Nov 29;167(1-2):135-44.
- Nemoto M, Tsunemitsu H, Murase H, Nambo Y, Sato S, Orita Y, Imagawa H, Bannai H, Tsujimura K, Yamanaka T, Matsumura T, Kondo T. Antibody response in vaccinated pregnant mares to recent G3BP[12] and G14P[12] equine rotaviruses. Acta Vet Scand 2012 Nov 6;54(1):63.
- Collins PJ, Cullinane A, Martella V, O'Shea H. Molecular characterization of equine rotavirus in Ireland. J Clin Microbiol 2008 Oct;46(10):3346-54.
- Dhama K, Chauhan RS, Mahendran M, Malik SV. Rotavirus diarrhea in bovines and other domestic animals. Vet Res Commun 2009 Jan;33(1):1-23.
- Gulati BR, Deepa R, Singh BK, Rao CD. Diversity in Indian equine rotaviruses: identification of genotype G10,P6[1] and G1 strains and a new VP7 genotype (G16) strain in specimens from diarrheic foals in India. J Clin Microbiol 2007 Mar;45(3):972-8.
- Uprety T, Soni S, Sreenivasan C, Hause BM, Naveed A, Ni S, Graves AJ, Morrow JK, Meade N, Mellits KH, Adam E, Kennedy MA, Wang D, Li F. Genetic and antigenic characterization of two diarrhoeicdominant rotavirus A genotypes G3P[12] and G14P[12] circulating in the global equine population. J Gen Virol 2024 Aug;105(8).
- Carossino M, Vissani MA, Barrandeguy ME, Balasuriya UBR, Parreño V. Equine Rotavirus A under the One Health Lens: Potential Impacts on Public Health. Viruses 2024 Jan 16;16(1).
Use Nutrition Calculator
Check if your horse's diet meets their nutrition requirements with our easy-to-use tool Check your horse's diet with our easy-to-use tool
Talk to a Nutritionist
Discuss your horse's feeding plan with our experts over a free phone consultation Discuss your horse's diet over a phone consultation
Submit Diet Evaluation
Get a customized feeding plan for your horse formulated by our equine nutritionists Get a custom feeding plan formulated by our nutritionists
