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Veterinary medicine and science2018; 4(4); 351-356; doi: 10.1002/vms3.126

Preimplantation factor modulates acute inflammatory responses of equine endometrium.

Abstract: Persistent mating-induced endometritis (PMIE) is a significant cause of mare infertility hence its treatment would advance the management of susceptible mares. Preimplantation factor (PIF) is secreted by viable embryos, including human, mouse and cattle, and is essential for maternal immune-tolerance without immune-suppression by modulating inflammation. This preliminary study aimed to test whether PIF exerts inflammatory-modulating properties upon equine endometrium challenged with Escherichia coli-derived lipopolysaccharide (LPS) using endometrial explant culture. Follicular (n = 3), luteal (n = 4), anoestrous (n = 4) and transitional (n = 4) stage endometrial explants were established and cultured in triplicate in either serum-free medium alone (control) or medium with; 50 or 100 nmol/L synthetic PIF (sPIF); 3 μg/mL LPS; LPS and 50 or 100 nmol/L sPIF; or scrambled PIF (PIFscr; same amino acid composition arranged in a different order). Media samples were collected at 24 and 72 h, representing acute and chronic inflammatory response. Radioimmunoassay determined Prostaglandin F (PGF ) as an inflammatory marker. The only significant observation was the abrogation of PGF response to LPS challenge by 100 nmol/L PIF for follicular stage tissue, 24 h after treatment. Further studies are therefore, warranted to realise PIF potential in managing PMIE.
© 2018 The Authors. Veterinary Medicine and Science Published by John Wiley & Sons Ltd.
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Publication Date: 2018-10-01 PubMed ID: 30273998PubMed Central: PMC6236140DOI: 10.1002/vms3.126Google Scholar: Lookup
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  • Journal Article
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  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research explores the potential of using Preimplantation factor (PIF) in regulating inflammation within the equine endometrium (horse womb tissue), particularly in response to challenges from bacteria-derived substances, as a way of treating mare infertility caused by Persistent mating-induced endometritis (PMIE).

Background

  • This study is based on the need to find better treatment options for Persistent mating-induced endometritis (PMIE), inflammation of the womb after mating, which is a chief cause of infertility in mares.
  • Previously, Preimplantation factor (PIF), a compound secreted by viable embryos in various species including humans, mice and cows, was found to play an essential role in promoting maternal immune-tolerance. It does this by moderating inflammation, without suppressing the immune system.

Aim and Methodology

  • The primary objective of the study was to test if synthetic PIF (sPIF) could modify inflammatory responses in the womb tissues of horses when confronted with Escherichia coli (E.coli)-derived lipopolysaccharide(LPS).
  • For this, endometrial explants (small pieces of endometrial tissue in culture) were prepared from horse womb tissues at various reproductive stages – follicular, luteal, anoestrous, and transitional.
  • These explants were exposed to either nothing (control), or sPIF (50 or 100 nmol/L), or LPS (3 µg/mL), or a combination of LPS and sPIF (50 or 100 nmol/L), or an altered form of PIF (scrambled PIF).

Results and conclusion

  • The only significant observation made was that a concentration of 100 nmol/L sPIF was able to halt the release of Prostaglandin F (PGF, a marker of inflammation) in the follicular-stage tissue when challenged with LPS, 24 hours after treatment.
  • This preliminary find calls for further research to fully understand the potential of PIF in managing the inflammatory problems associated with horse reproduction and consequently, infertility.

Cite This Article

APA
Nash DM, Paddison J, Davies Morel MCG, Barnea ER. (2018). Preimplantation factor modulates acute inflammatory responses of equine endometrium. Vet Med Sci, 4(4), 351-356. https://doi.org/10.1002/vms3.126

Publication

Veterinary medicine and science
ISSN: 2053-1095
NlmUniqueID: 101678837
Country: England
Language: English
Volume: 4
Issue: 4
Pages: 351-356

Researcher Affiliations

Nash, Deborah M
  • Institute of Biological, Environmental and Rural Sciences (IBERS), Aberystwyth University, Penglais, Aberystwyth, UK.
Paddison, Jennifer
  • Institute of Biological, Environmental and Rural Sciences (IBERS), Aberystwyth University, Penglais, Aberystwyth, UK.
Davies Morel, Mina C G
  • Institute of Biological, Environmental and Rural Sciences (IBERS), Aberystwyth University, Penglais, Aberystwyth, UK.
Barnea, Eytan R
  • Society for the Investigation of Early Pregnancy, Cherry Hill, New Jersey, USA.

MeSH Terms

  • Animals
  • Dinoprost / metabolism
  • Endometrium / drug effects
  • Female
  • Horses
  • Inflammation / drug therapy
  • Inflammation / metabolism
  • Inflammation / veterinary
  • Pregnancy Proteins / metabolism
  • Tissue Culture Techniques

Grant Funding

  • Biotechnology and Biological Sciences Research Council

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Citations

This article has been cited 4 times.
  1. Monteiro de Barros MR, Davies-Morel MCG, Mur LAJ, Creevey CJ, Alison RH, Nash DM. Characterization of an Ex Vivo Equine Endometrial Tissue Culture Model Using Next-Generation RNA-Sequencing Technology. Animals (Basel) 2021 Jul 3;11(7).
    doi: 10.3390/ani11071995pubmed: 34359123google scholar: lookup
  2. Santos ED, Moindjie H, Sérazin V, Arnould L, Rodriguez Y, Fathallah K, Barnea ER, Vialard F, Dieudonné MN. Preimplantation factor modulates trophoblastic invasion throughout the decidualization of human endometrial stromal cells. Reprod Biol Endocrinol 2021 Jun 27;19(1):96.
    doi: 10.1186/s12958-021-00774-5pubmed: 34176510google scholar: lookup
  3. Wonfor RE, Creevey CJ, Natoli M, Hegarty M, Nash DM, Rose MT. Interaction of preimplantation factor with the global bovine endometrial transcriptome. PLoS One 2020;15(12):e0242874.
    doi: 10.1371/journal.pone.0242874pubmed: 33284816google scholar: lookup
  4. Merhi Z, Garg B, Moseley-LaRue R, Moseley AR, Smith AH, Zhang J. Ozone therapy: a potential therapeutic adjunct for improving female reproductive health. Med Gas Res 2019 Apr-Jun;9(2):101-105.
    doi: 10.4103/2045-9912.260652pubmed: 31249259google scholar: lookup
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