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Home / Equine Research Bank / J Med Virol / Preliminary characterisation of torovirus-like particles of ...
Journal of medical virology1986; 20(1); 67-78; doi: 10.1002/jmv.1890200109

Preliminary characterisation of torovirus-like particles of humans: comparison with Berne virus of horses and Breda virus of calves.

Abstract: Pleomorphic virus-like particles have been observed by electron microscopy in the faeces of children and adults with diarrhoea. Some of these particles were approximately 100 nm in diameter and had a "fringe" of closely applied peplomers approximately 10 nm long; they closely resembled Berne virus of horses and Breda virus of calves, the two representatives of a newly proposed family called the Toroviridae. In one sample a toroidal nucleoprotein-like structure was observed within the particles. For two samples a buoyant density of 1.14 g/ml was determined by centrifugation through a sucrose density gradient. One sample possessed a haemagglutinin for rat erythrocytes. The serological relationship between these different viruses was observed by immune electron microscopy, haemagglutination inhibition, and serum neutralisation. The role of these virus-like particles as candidate pathogens of humans is discussed.
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Publication Date: 1986-09-01 PubMed ID: 3093635PubMed Central: PMC7166937DOI: 10.1002/jmv.1890200109Google Scholar: Lookup
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  • Comparative Study
  • Journal Article
  • Research Support
  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research investigates virus-like particles in human feces, specifically focusing on their potential relationship to the horse’s Berne virus and the cow’s Breda virus, part of the newly identified Toroviridae family. The researchers discuss the physical properties of these particles and their possible role in causing human illnesses.

Observed Virus-like Particles

  • Researchers observed pleomorphic (shapeshifting) virus-like particles in the feces of adults and children suffering from diarrhea using electron microscopy.
  • Some of the observed particles were approximately 100 nanometers (nm) in diameter, with a “fringe” of closely applied peplomers around 10 nm long.
  • The particles resembled the Berne virus found in horses and the Breda virus found in calves. Both of these viruses belong to the new family named Toroviridae.

Physical Properties of the Particles

  • In one sample, a round-ribbon-shaped nucleoprotein-like structure was observed within the particles.
  • Centrifugation through a sucrose density gradient revealed a buoyant density of 1.14 g/ml for two samples, contributing to the detailed physical characterization of these particles.
  • One of the samples had a haemagglutinin, a substance causing red blood cells to agglutinate, for rat erythrocytes, suggesting possible compatibility with animal cells.

Serological Relationship with Other Viruses

  • The study looked into the serological relationship (the relationship based on detection of antibodies in the serum) between these virus-like particles and other viruses.
  • The researchers used methods such as immune electron microscopy, haemagglutination inhibition, and serum neutralisation to observe these serological relationships.
  • The understanding of such relationships helps map the spread and potential hazards of these viruses.

Potential Pathogenic Role in Humans

  • The researchers discuss the role that these virus-like particles might play as pathogenic candidates in humans, suggesting that they could potentially cause disease.
  • This preliminary characterization lays the foundation for further studies to understand the potential risks these particles pose to human health.

Cite This Article

APA
Beards GM, Brown DW, Green J, Flewett TH. (1986). Preliminary characterisation of torovirus-like particles of humans: comparison with Berne virus of horses and Breda virus of calves. J Med Virol, 20(1), 67-78. https://doi.org/10.1002/jmv.1890200109

Publication

Journal of medical virology
ISSN: 0146-6615
NlmUniqueID: 7705876
Country: United States
Language: English
Volume: 20
Issue: 1
Pages: 67-78

Researcher Affiliations

Beards, G M
    Brown, D W
      Green, J
        Flewett, T H

          MeSH Terms

          • Adult
          • Animals
          • Cattle
          • Centrifugation, Density Gradient
          • Child
          • Feces / microbiology
          • Hemagglutination Inhibition Tests
          • Hemagglutination Tests
          • Horses
          • Humans
          • Microscopy, Electron
          • Neutralization Tests
          • Viruses, Unclassified / immunology
          • Viruses, Unclassified / ultrastructure

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