English
French
Preliminary evaluation of hepatitis A virus cell receptor 1/kidney injury molecule 1 in healthy horses treated with phenylbutazone.
Abstract: To investigate if hepatitis A virus cell receptor 1/kidney injury molecule 1 (HAVCR1/KIM1) in urine is detectable concurrently with increases in serum creatinine concentrations in horses receiving a recommended dose of phenylbutazone (PBZ) for 7 days. Methods: Preliminary study. Methods: Ten clinically healthy horses with normal physical examination and laboratory work were randomly assigned to PBZ or placebo groups (5 each). The PBZ group received PBZ at 4.4 mg/kg mixed with corn syrup orally every 12 hours. The placebo group received corn syrup orally every 12 hours. Both groups were treated for 7 days. Kidney ultrasonography was performed, and venous blood and urine samples were collected prior to commencement and at the end of treatment. Samples from 1 additional healthy horse, 3 horses with acute kidney failure, and 1 horse with chronic kidney failure were also evaluated. Results: None of the 10 horses had detectable HAVCR1/KIM1 in urine at baseline. Serum creatinine concentrations in placebo group did not increase, and HAVCR1/KIM1 was undetectable in urine. At the end of treatment, 3 of 5 horses receiving PBZ developed increases in serum creatinine of >26.5 μmol/L (>0.3 mg/dL), and HAVCR1/KIM1 was detectable in urine, despite normal findings on kidney ultrasonography in all horses. Conclusions: HAVCR1/KIM1 is detectable in urine and is associated with increases in serum creatinine concentrations of >26.5 μmol/L in horses following treatment with PBZ for 7 consecutive days. Thus, HAVCR1/KIM1 might aid in the early detection of acute kidney injury in horses.
© Veterinary Emergency and Critical Care Society 2023.
Publication Date: 2023-07-12 PubMed ID: 37436880DOI: 10.1111/vec.13314Google Scholar: Lookup The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
- Journal Article
Summary
This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.
This research pertains to a study investigating if the hepatitis A virus cell receptor 1/kidney injury molecule 1 (HAVCR1/KIM1) in horses’ urine can be detected at the same time as increases in serum creatinine concentrations, which are an indication of kidney damage. This observation was made in horses given a standard dose of phenylbutazone (PBZ), a nonsteroidal anti-inflammatory drug, for seven days.
Method
- The study involved ten healthy horses that were randomly assigned to either the PBZ group or the placebo group with five horses in each. Horses in the PBZ group received a PBZ dosage at 4.4 mg/kg mixed with corn syrup orally every 12 hours. Horses in the placebo group received corn syrup orally every 12 hours. The varieties of treatment continued for seven days.
- Kidney ultrasound was performed on all horses, and vein blood and urine samples were collected before and after treatment. For further reference, samples from one healthy horse, three horses with acute kidney failure, and one horse with chronic kidney failure were also examined.
Results
- At the onset of the study, none of the ten horses had detectable HAVCR1/KIM1 in their urine. For horses in the placebo group, their serum creatinine concentrations did not increase, and HAVCR1/KIM1 was not detectable in their urine.
- However, at the end of the seven-day treatment, three of the five horses receiving PBZ had increased serum creatinine concentrations of more than 26.5 μmol/L and contained detectable HAVCR1/KIM1 in their urine. It is noteworthy that normal findings were recorded for all horses following a kidney ultrasound.
Conclusion
- The outcome of the research associates the detectability of HAVCR1/KIM1 in urine with an observed increase in serum creatinine concentrations of more than 26.5 μmol/L in horses that have been treated with PBZ for seven consecutive days.
- Therefore, this suggests that the presence of HAVCR1/KIM1 in urine could aid in the early detection of acute kidney injury in horses.
Cite This Article
APA
Costa LRR, Swiderski C, Palm C, Aleman M.
(2023).
Preliminary evaluation of hepatitis A virus cell receptor 1/kidney injury molecule 1 in healthy horses treated with phenylbutazone.
J Vet Emerg Crit Care (San Antonio), 33(4), 481-486.
https://doi.org/10.1111/vec.13314 Publication
Researcher Affiliations
- Department of Medicine and Epidemiology, School of Veterinary Medicine, University of California, Davis, Davis, California, USA.
- Department of Pathobiology and Population Medicine, School of Veterinary Medicine, Mississippi State University, Mississippi State, Mississippi, USA.
- Department of Medicine and Epidemiology, School of Veterinary Medicine, University of California, Davis, Davis, California, USA.
- Department of Medicine and Epidemiology, School of Veterinary Medicine, University of California, Davis, Davis, California, USA.
References
This article includes 17 references
- Bellomo R, Kellum JA, Ronco C. Acute kidney injury. Lancet. 2012;380:756-766.
- Schott HC, 2nd, Esser MM. The sick adult horse: renal clinical pathologic testing and urinalysis. Vet Clin North Am Equine Pract. 2020;36:121-134.
- Kopecny L, Palm CA, Skorupski KA, etu00a0al. Risk factors associated with progressive increases in serum creatinine concentrations in cats with cancer receiving doxorubicin. J Vet Intern Med. 2020;34:2048-2055.
- Siwinska N, Zak A, Slowikowska M, etu00a0al. Serum symmetric dimethylarginine concentration in healthy horses and horses with acute kidney injury. BMC Vet Res. 2020;16:396.
- Bayless RL, Moore AR, Hassel DM, etu00a0al. Equine urinary N-acetyl-u03b2-D-glucosaminidase assay validation and correlation with other markers of kidney injury. J Vet Diagn Invest. 2019;31:688-695.
- Jacobsen S, Berg LC, Tvermose E, etu00a0al. Validation of an ELISA for detection of neutrophil gelatinase-associated lipocalin (NGAL) in equine serum. Vet Clin Pathol. 2018;47:603-607.
- Siwinska N, Zak A, Paslawska U. Detecting acute kidney injury in horses by measuring the concentration of symmetric dimethylarginine in serum. Acta Vet Scand. 2021;63:3.
- Han WK, Bailly V, Abichandani R, etu00a0al. Kidney injury molecule-1 (KIM-1): a novel biomarker for human renal proximal tubule injury. Kidney Int. 2002;62:237-244.
- Chaturvedi S, Farmer T, Kapke GF. Assay validation for KIM-1: human urinary renal dysfunction biomarker. Int J Biol Sci. 2009;5:128-134.
- Waanders F, van Timmeren MM, Stegeman CA, etu00a0al. Kidney injury molecule-1 in renal disease. J Pathol. 2019;220:7-16.
- Gu YZ, Vlasakova K, Troth SP, etu00a0al. Performance assessment of new urinary translational safety biomarkers of drug-induced renal tubular injury in tenofovir-treated cynomolgus monkeys and beagle dogs. Toxicol Pathol. 2018;46:553-563.
- Fogle C, Davis JL, Yechuri B, etu00a0al. Ex vivo COX-1 and COX-2 inhibition in equine blood by phenylbutazone, flunixin meglumine, meloxicam and firocoxib: informing clinical NSAID selection. Equine Vet Educ. 2020;33:198-207.
- MacAllister CG, Morgan SJ, Borne AT, etu00a0al. Comparison of adverse effects of phenylbutazone, flunixin meglumine, and ketoprofen in horses. J Am Vet Med Assoc. 1993;202:71-77.
- Gunson DE, Soma LR. Renal papillary necrosis in horses after phenylbutazone and water deprivation. Vet Pathol. 1983;20:603-610.
- Ramirez S, Seahorn TL, Williams J. Renal medullary rim sign in 2 adult quarter horses. Can Vet J. 1998;39:647-649.
- Ichimura T, Bonventre JV, Wei H, etu00a0al. Kidney injury molecule-1 (KIM-1), a putative epithelial cell adhesion molecule containing a novel immunoglobulin domain, is up-regulated in renal cells after injury. J Biol Chem. 1998;273:4135-4142.
- Geor RJ. Acute renal failure in horses. Vet Clin North Am Equine Pract. 2007;23:577-591.
Citations
This article has been cited 0 times.Secure Payments
Satisfaction Guarantee
We offer a 60-day guarantee to ensure you are completely satisfied.
Fast & Free Shipping
We provide fast and free shipping on all orders over $40.