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Home / Equine Research Bank / J Vet Emerg Crit Care (San Antonio) / Preliminary evaluation of reference intervals for a point-of...
Journal of veterinary emergency and critical care (San Antonio, Tex. : 2001)2023; doi: 10.1111/vec.13317

Preliminary evaluation of reference intervals for a point-of-care viscoelastic coagulation monitor (VCM Vet) in healthy adult horses.

Abstract: To evaluate a point-of-care viscoelastic coagulation monitor (VCM Vet) for use in horses by assessing variability between devices and establish reference intervals (RIs) for healthy adult horses. Methods: Prospective observational study. Methods: Two university teaching hospitals. Methods: Healthy adult horses (n = 68). Methods: None. Results: Blood collected by direct jugular venipuncture was applied directly from the syringe into 2 VCM Vet cassettes to establish coefficients of variation (CVs) and RIs for reported parameters of clotting time (CT), clot formation time (CFT), alpha angle, amplitude at 10 and 20 minutes, maximum clot firmness, and lysis index at 30 and 45 minutes. CVs for each parameter were within clinical tolerance. There was a significant difference in CT between institutions (P < 0.001). Differences in CV were found between institutions for CT (P = 0.003) and CFT (P = 0.01). Healthy horse RIs were calculated for the overall data set and each individual institution. Calculated RIs were as follows: CT, 255.6-1233.9 seconds; CFT, 89.4-581 seconds; alpha angle, 11.4-53.6°; maximum clot firmness, 18-37.7; lysis index at 30 minutes, 97.3%-102.1%; lysis index at 45 minutes, 80.8%-103.3%; amplitude at 10 minutes, 8.7-28.3; and amplitude at 20 minutes, 17.4-35.7. Conclusions: VCM Vet is a repeatable and practical option for rapid point-of-care assessment of hemostasis in horses but has a wide RI and is susceptible to variability. Establishment of institution-specific RIs is recommended.
© Veterinary Emergency and Critical Care Society 2023.
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Publication Date: 2023-08-10 PubMed ID: 37561043DOI: 10.1111/vec.13317Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research aimed to evaluate the use of a point-of-care viscoelastic coagulation monitor (VCM Vet) in horses. The findings show that the VCM Vet can effectively monitor blood coagulation in horses but there is potential for variability and institution-specific reference intervals should be considered.

Experiment Methodology

  • The authors took this study as a prospective observational experiment, conducted in two university teaching hospitals.
  • The experiment relied on the blood samples from 68 healthy adult horses. Blood was collected through direct jugular venipuncture and applied straight from the syringe into two VCM Vet cassettes.
  • The primary aim was to assess the possible variability between devices and establish reference intervals (RIs) for the clotted blood of these healthy adult horses.

Collected Data and Findings

  • Several parameters were monitored during this study, including clotting time (CT), clot formation time (CFT), alpha angle, the amplitude at 10 and 20 minutes, maximum clot firmness, and lysis index at 30 and 45 minutes.
  • The research team established coefficients of variation (CVs) and the RIs for the parameters observed. According to the results, CVs for each parameter were found to be within clinically acceptable tolerance.
  • However, there was a noted significant difference in CT between institutions, and differences were identified in CVs of CT and CFT across the two institutions.
  • Based on the collected overall data, the team computed the RIs. For instance, the RIs for CT, CFT, alpha angle were found to be 255.6-1233.9 seconds, 89.4-581 seconds, and 11.4-53.6° respectively.

Conclusion and Suggestions

  • The study concluded that VCM Vet offers repeatable results and is a practical choice for rapid point-of-care assessment of hemostasis in horses.
  • But, it’s susceptible to variability, as reflected in the wide-ranging RIs. Hence, the study recommends establishing institution-specific reference intervals to mitigate these discrepancies in the future.

Cite This Article

APA
Bishop RC, Kemper AM, Burges JW, Jandrey KE, Wilkins PA. (2023). Preliminary evaluation of reference intervals for a point-of-care viscoelastic coagulation monitor (VCM Vet) in healthy adult horses. J Vet Emerg Crit Care (San Antonio). https://doi.org/10.1111/vec.13317

Publication

Journal of veterinary emergency and critical care (San Antonio, Tex. : 2001)
ISSN: 1476-4431
NlmUniqueID: 101152804
Country: United States
Language: English

Researcher Affiliations

Bishop, Rebecca C
  • Department of Veterinary Clinical Medicine, University of Illinois Urbana-Champaign, Urbana, Illinois, USA.
Kemper, Ann M
  • Department of Veterinary Clinical Medicine, University of Illinois Urbana-Champaign, Urbana, Illinois, USA.
Burges, Julie W
  • William R. Pritchard Veterinary Medical Teaching Hospital, School of Veterinary Medicine, University of California, Davis, Davis, California, USA.
Jandrey, Karl E
  • William R. Pritchard Veterinary Medical Teaching Hospital, School of Veterinary Medicine, University of California, Davis, Davis, California, USA.
Wilkins, Pamela A
  • Department of Veterinary Clinical Medicine, University of Illinois Urbana-Champaign, Urbana, Illinois, USA.

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