Preliminary investigations on the effects of a Strongylus vulgaris larval extract, mononuclear factors and platelet factors on equine smooth muscle cells in vitro.
Abstract: Factors involved in the proliferation of equine vascular smooth muscle cells were studied in vitro. The most prominent proliferative responses in cultured vascular smooth muscle cells were induced by Strongylus vulgaris larval antigen extract (LAE) and platelet-derived factors. Less significant proliferative responses were obtained with conditioned media from S. vulgaris LAE stimulated and from unstimulated equine mononuclear leukocytes. Additionally, vascular smooth muscle cells exposed to S. vulgaris LAE developed numerous perinuclear vacuoles and were more spindle-shaped than control or smooth muscle cells exposed to other factors. Equine mononuclear leukocytes exposed to LAE developed prominent morphological changes, including enlargement, clumping and increased numbers of mitotic figures.
Publication Date: 1989-01-01 PubMed ID: 2631384DOI: 10.1007/BF00402572Google Scholar: Lookup
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- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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The research explored the impact of certain factors on the proliferation of equine vascular smooth muscle cells. It was found that a larval antigen extract from parasitic worm Strongylus vulgaris and platelet-derived factors led to significant cell proliferation. The study also uncovered changes in the morphology of these cells and their reactions toward equine mononuclear leukocytes.
Stimulus for Vascular Smooth Muscle Cell Proliferation
- The research initially aimed at investigating the factors that instigate the proliferation of equine vascular smooth muscle cells.
- The most potent proliferative responses were prompted by a larval antigen extract (LAE) from the parasite Strongylus vulgaris and factors derived from platelets.
- The study noted that these factors caused the cells to proliferate more prominently than others.
Reactions to S. vulgaris LAE
- Particularly, cells exposed to the S. vulgaris LAE displayed a variety of morphological changes including the development of numerous vacuoles around the cell nucleus.
- Moreover, these cells were observed to become more spindle-shaped compared to the control cells or cells exposed to other factors.
- Such changes suggest that S. vulgaris LAE has a significant effect on the cell development and shape.
Impact on Equine Mononuclear Leukocytes
- Furthermore, the study observed changes in equine mononuclear leukocytes when these cells were exposed to the S. vulgaris LAE. These changes included enlargement, the tendency to clump together and an increased number of mitotic figures.
- The fact that S. vulgaris LAE can stimulate such strong reactions in equine mononuclear leukocytes indicates its high biological activity and potential implications for horse health and diseases.
Less Significant Proliferative Responses
- Notably, less significant proliferative responses were obtained when the cells were exposed to conditioned media from S. vulgaris LAE stimulated and from unstimulated equine mononuclear leukocytes.
- This implies that the latter factors were not as efficient in promoting the proliferation of equine smooth muscle cells as S. vulgaris LAE and platelet-derived factors.
Cite This Article
APA
Morgan SJ, Storts RW, Stromberg PC, Sowa BA, Lay JC.
(1989).
Preliminary investigations on the effects of a Strongylus vulgaris larval extract, mononuclear factors and platelet factors on equine smooth muscle cells in vitro.
Vet Res Commun, 13(6), 479-489.
https://doi.org/10.1007/BF00402572 Publication
Researcher Affiliations
- Department of Veterinary Pathology, Texas A & M University, College Station 77843.
MeSH Terms
- Animals
- Antigens, Helminth / isolation & purification
- Antigens, Helminth / pharmacology
- Arteritis / etiology
- Arteritis / pathology
- Arteritis / veterinary
- Blood Platelets / metabolism
- Cell Division
- Cells, Cultured
- Horses
- Leukocytes, Mononuclear / immunology
- Leukocytes, Mononuclear / metabolism
- Lymphocyte Activation
- Muscle, Smooth, Vascular / pathology
- Strongyle Infections, Equine / pathology
- Strongylus
References
This article includes 18 references
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Citations
This article has been cited 1 times.- Morgan SJ, Van Houten DS. The ultrastructure of Strongylus vulgaris-mediated equine chronic mesenteric arteritis.. Vet Res Commun 1990;14(1):41-6.
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