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Home / Equine Research Bank / Reprod Domest Anim / Premature centromere division (PCD) identified in a hucul ma...
Reproduction in domestic animals = Zuchthygiene2019; 55(2); 248-251; doi: 10.1111/rda.13605

Premature centromere division (PCD) identified in a hucul mare with reproductive difficulties.

Abstract: A hucul mare with reproductive abnormalities was examined during karyotype analysis. The karyotype was analysed based on evaluation of 860 metaphase plates in chromosome preparations. The use of fluorescence in situ hybridization (FISH) with an X chromosome painting probe showed premature X chromosome separation in 9.5% cases of examined chromosome spreads. In this report, we present the first identify premature centromere division (PCD) as a possible cause of abnormal X chromosome morphology in horses and as a probable cause of reproductive difficulties.
© 2019 Blackwell Verlag GmbH.
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Publication Date: 2019-12-26 PubMed ID: 31834942DOI: 10.1111/rda.13605Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research presents the case of a hucul mare with reproductive issues where they found abnormal X chromosome division. This abnormality, identified for the first time in horses, could be the underlying cause of the mare’s reproductive problems.

Introduction to the Study

  • The study primarily focused on a particular mare of the hucul breed that was experiencing reproductive issues.
  • This prompted the researchers to conduct a karyotype analysis on the mare to understand what could be leading to these complications.

Karyotype Analysis and Findings

  • As part of the karyotype analysis, the researchers examined 860 metaphase plates in chromosome preparations to explore any potential abnormalities.
  • They used fluorescence in situ hybridization (FISH), a technique that allows for visualization of specific regions on the chromosome with a high degree of specificity.
  • During their analysis using FISH, they noticed premature separation of the X chromosome in about 9.5% of the chromosome spreads examined. This anomaly was identified as premature centromere division (PCD), a phenomenon not previously observed in horses.

Implications of the Findings

  • The study findings suggest PCD as a possible cause for abnormal X chromosome morphology in horses, which is a significant breakthrough.
  • Another critical implication is that PCD could be a likely reason for the observed reproductive difficulties in the mare under study. It opens up new avenues for research into the causes behind reproductive issues in horses.

Conclusion

  • This research helps increase our understanding of the genetic characteristics and abnormalities that can affect equine reproduction. It will be a crucial pointer for future research in this area. Nevertheless, additional studies are required to confirm these findings and explore the broader impacts of PCD on horse reproduction.

Cite This Article

APA
Witarski W, Kij B, Nowak A, Bugno-Poniewierska M. (2019). Premature centromere division (PCD) identified in a hucul mare with reproductive difficulties. Reprod Domest Anim, 55(2), 248-251. https://doi.org/10.1111/rda.13605

Publication

Reproduction in domestic animals = Zuchthygiene
ISSN: 1439-0531
NlmUniqueID: 9015668
Country: Germany
Language: English
Volume: 55
Issue: 2
Pages: 248-251

Researcher Affiliations

Witarski, Wojciech
  • Department of Animal Genetics, National Institute of Animal Production, Balice, Poland.
Kij, Barbara
  • Department of Animal Reproduction, Anatomy and Genomics, University of Agriculture in Krakow, Krakow, Poland.
Nowak, Agnieszka
  • Department of Animal Reproduction, Anatomy and Genomics, University of Agriculture in Krakow, Krakow, Poland.
Bugno-Poniewierska, Monika
  • Department of Animal Reproduction, Anatomy and Genomics, University of Agriculture in Krakow, Krakow, Poland.

MeSH Terms

  • Animals
  • Centromere / genetics
  • Female
  • Horses / genetics
  • Infertility, Female / genetics
  • Infertility, Female / veterinary
  • Sex Chromosome Aberrations / veterinary
  • X Chromosome / genetics

Grant Funding

  • NCBiR:BIOSTRATEG2/297267/14/NCBR/2016 / National Centre for Research and Development,

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This article includes 22 references
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Citations

This article has been cited 1 times.
  1. Bugno-Poniewierska M, Raudsepp T. Horse Clinical Cytogenetics: Recurrent Themes and Novel Findings. Animals (Basel) 2021 Mar 16;11(3).
    doi: 10.3390/ani11030831pubmed: 33809432google scholar: lookup
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