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Home / Equine Research Bank / Brain Pathol / Presence of CD4+ and CD8+ T cells and expression of MHC clas...
Brain pathology (Zurich, Switzerland)1995; 5(3); 223-230; doi: 10.1111/j.1750-3639.1995.tb00598.x

Presence of CD4+ and CD8+ T cells and expression of MHC class I and MHC class II antigen in horses with Borna disease virus-induced encephalitis.

Abstract: Tissues from 9 horses and 1 donkey suffering from natural Borna disease were investigated immunomorphologically. Lymphocytic inflammatory reactions and increased expressions of MHC class I and class II antigen were found in the brain as well as in the trigeminal and olfactory system. Perivascular inflammatory infiltrates were dominated by CD4+ T cells, whereas the majority of CD8+ T cells were disseminated intraparenchymally. No evidence of inflammation was found in the retina. Borna disease virus proteins and nucleic acids were present in the hippocampus, thalamus and medulla oblongata in all 10 animals, in the cerebral cortex, retina, trigeminal ganglion and nerve in 9, in the olfactory epithelium in 6 and in roots and proximal parts of large peripheral nerves in 3. No evidence of infection was found in the autonomic nervous system, lung, heart, liver, kidney or gut. BDV- proteins and nucleic acids were even more abundant in the trigeminal system than in the olfactory system, suggesting that infection may have occurred via the trigeminal nerve.
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Publication Date: 1995-07-01 PubMed ID: 8520721DOI: 10.1111/j.1750-3639.1995.tb00598.xGoogle Scholar: Lookup
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  • Journal Article
  • Research Support
  • Non-U.S. Gov't
  • Research Support
  • U.S. Gov't
  • P.H.S.

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research investigates the immunological response in horses affected by Borna disease, characterized by encephalitis. The study analyzes the presence and distribution of T cell populations and MHC class I and II antigens in the nervous system of affected animals, and the potential pathways of the virus infection.

Research Methodology and Materials

  • The tissue samples used in the study were taken from nine horses and one donkey that were affected by natural Borna disease.
  • The researchers conducted an immunomorphological investigation to assess the cellular and tissue structural alterations associated with the immune response.

Key Findings

  • Increased expressions of MHC class I and class II antigen and lymphocytic inflammatory reactions were detected in the brain, and the olfactory and trigeminal systems in the animals.
  • CD4+ T cells predominantly formed perivascular inflammatory infiltrates, while the majority of CD8+ T cells were scattered within the nerve tissue (intraparenchymally).
  • No signs of inflammation were noted in the retina of the animals.
  • Borna disease virus proteins and nucleic acids were found in numerous parts of the nervous system, including the hippocampus, thalamus, and medulla oblongata in all 10 animals.
  • The expression of Borna disease virus proteins and nucleic acid was most abundant in the horses’ trigeminal system—an observation leading the researchers to suggest that infection may have occurred via the trigeminal nerve.

Significant Implications

  • The finding of no inflammation in the retina despite Borna virus presence indicates a localized immune response in different parts of the nervous system.
  • The expression and distribution of different T cell types might reveal the pathophysiological events in Borna disease and the immune mechanisms at play during viral encephalitis.
  • The study reveals new insights about the pathway of infection, suggesting the trigeminal nerve as a significant route for Borna virus, expanding our understanding of the disease’s spread and potentially informing strategies for therapeutic interventions and disease control.

Cite This Article

APA
Bilzer T, Planz O, Lipkin WI, Stitz L. (1995). Presence of CD4+ and CD8+ T cells and expression of MHC class I and MHC class II antigen in horses with Borna disease virus-induced encephalitis. Brain Pathol, 5(3), 223-230. https://doi.org/10.1111/j.1750-3639.1995.tb00598.x

Publication

Brain pathology (Zurich, Switzerland)
ISSN: 1015-6305
NlmUniqueID: 9216781
Country: Switzerland
Language: English
Volume: 5
Issue: 3
Pages: 223-230

Researcher Affiliations

Bilzer, T
  • Institut für Neuropathologie, Heinrich-Heine-Universität, Düsseldorf, Germany.
Planz, O
    Lipkin, W I
      Stitz, L

        MeSH Terms

        • Animals
        • Borna Disease / immunology
        • Borna Disease / virology
        • Brain / immunology
        • CD4-Positive T-Lymphocytes / immunology
        • CD8-Positive T-Lymphocytes / immunology
        • Equidae / immunology
        • Histocompatibility Antigens / blood
        • Histocompatibility Antigens Class I / blood
        • Histocompatibility Antigens Class II / blood
        • Horse Diseases / immunology
        • Horses
        • Inflammation / immunology

        Grant Funding

        • NS-29425 / NINDS NIH HHS

        Citations

        This article has been cited 26 times.
        1. Sighencea MG, Trifu SC. Unravelling the Viral Hypothesis of Schizophrenia: A Comprehensive Review of Mechanisms and Evidence. Int J Mol Sci 2025 Aug 1;26(15).
          doi: 10.3390/ijms26157429pubmed: 40806558google scholar: lookup
        2. Vollmuth Y, Jungbäck N, Grochowski P, Mögele T, Stark L, Zarrabi NS, Schlegel J, Schaller T, Märkl B, Matiasek K, Liesche-Starnecker F. Mapping Bornavirus encephalitis-A comparative study of viral spread and immune response in human and animal dead-end hosts. PLoS Pathog 2025 Aug;21(8):e1013400.
          doi: 10.1371/journal.ppat.1013400pubmed: 40758738google scholar: lookup
        3. Ebinger A, Santos PD, Pfaff F, Dürrwald R, Kolodziejek J, Schlottau K, Ruf V, Liesche-Starnecker F, Ensser A, Korn K, Ulrich R, Fürstenau J, Matiasek K, Hansmann F, Seuberlich T, Nobach D, Müller M, Neubauer-Juric A, Suchowski M, Bauswein M, Niller HH, Schmidt B, Tappe D, Cadar D, Homeier-Bachmann T, Haring VC, Pörtner K, Frank C, Mundhenk L, Hoffmann B, Herms J, Baumgärtner W, Nowotny N, Schlegel J, Ulrich RG, Beer M, Rubbenstroth D. Lethal Borna disease virus 1 infections of humans and animals - in-depth molecular epidemiology and phylogeography. Nat Commun 2024 Sep 10;15(1):7908.
          doi: 10.1038/s41467-024-52192-xpubmed: 39256401google scholar: lookup
        4. Kotsiri I, Resta P, Spyrantis A, Panotopoulos C, Chaniotis D, Beloukas A, Magiorkinis E. Viral Infections and Schizophrenia: A Comprehensive Review. Viruses 2023 Jun 9;15(6).
          doi: 10.3390/v15061345pubmed: 37376644google scholar: lookup
        5. Rubbenstroth D. Avian Bornavirus Research-A Comprehensive Review. Viruses 2022 Jul 11;14(7).
          doi: 10.3390/v14071513pubmed: 35891493google scholar: lookup
        6. Komorizono R, Sassa Y, Horie M, Makino A, Tomonaga K. Evolutionary Selection of the Nuclear Localization Signal in the Viral Nucleoprotein Leads to Host Adaptation of the Genus Orthobornavirus. Viruses 2020 Nov 11;12(11).
          doi: 10.3390/v12111291pubmed: 33187187google scholar: lookup
        7. More S, Bøtner A, Butterworth A, Calistri P, Depner K, Edwards S, Garin-Bastuji B, Good M, Gortázar Schmidt C, Michel V, Miranda MA, Nielsen SS, Raj M, Sihvonen L, Spoolder H, Stegeman JA, Thulke HH, Velarde A, Willeberg P, Winckler C, Baldinelli F, Broglia A, Dhollander S, Beltrán-Beck B, Kohnle L, Bicout D. Assessment of listing and categorisation of animal diseases within the framework of the Animal Health Law (Regulation (EU) No 2016/429): Borna disease. EFSA J 2017 Jul;15(7):e04951.
          doi: 10.2903/j.efsa.2017.4951pubmed: 32625602google scholar: lookup
        8. Yanai M, Kojima S, Sakai M, Komorizono R, Tomonaga K, Makino A. ADAR2 Is Involved in Self and Nonself Recognition of Borna Disease Virus Genomic RNA in the Nucleus. J Virol 2020 Feb 28;94(6).
          doi: 10.1128/JVI.01513-19pubmed: 31852792google scholar: lookup
        9. Tappe D, Schmidt-Chanasit J, Rauch J, Allartz P, Herden C. Immunopathology of Fatal Human Variegated Squirrel Bornavirus 1 Encephalitis, Germany, 2011-2013. Emerg Infect Dis 2019 Jun;25(6):1058-1065.
          doi: 10.3201/eid2506.181082pubmed: 31107210google scholar: lookup
        10. . Bornavirus : Stellungnahmen des Arbeitskreises Blut des Bundesministeriums für Gesundheit. Bundesgesundheitsblatt Gesundheitsforschung Gesundheitsschutz 2019 Apr;62(4):519-532.
          doi: 10.1007/s00103-019-02904-2pubmed: 30820613google scholar: lookup
        11. Shatizadeh-Malekshahi S, Ahmadkhaniha HR, Kiani SJ, Nejati A, Janani L, Yavarian J. No molecular evidence of Borna disease virus among schizophrenia and bipolar disorder patients in Iran. Iran J Microbiol 2017 Apr;9(2):112-118.
          pubmed: 29214003
        12. Delcambre GH, Liu J, Herrington JM, Vallario K, Long MT. Immunohistochemistry for the detection of neural and inflammatory cells in equine brain tissue. PeerJ 2016;4:e1601.
          doi: 10.7717/peerj.1601pubmed: 26855862google scholar: lookup
        13. Baur K, Rauer M, Richter K, Pagenstecher A, Götz J, Hausmann J, Staeheli P. Antiviral CD8 T cells recognize borna disease virus antigen transgenically expressed in either neurons or astrocytes. J Virol 2008 Mar;82(6):3099-108.
          doi: 10.1128/JVI.02479-07pubmed: 18184705google scholar: lookup
        14. Richter K, Baur K, Ackermann A, Schneider U, Hausmann J, Staeheli P. Pathogenic potential of borna disease virus lacking the immunodominant CD8 T-cell epitope. J Virol 2007 Oct;81(20):11187-94.
          doi: 10.1128/JVI.00742-07pubmed: 17686872google scholar: lookup
        15. Schindler AR, Vögtlin A, Hilbe M, Puorger M, Zlinszky K, Ackermann M, Ehrensperger F. Reverse transcription real-time PCR assays for detection and quantification of Borna disease virus in diseased hosts. Mol Cell Probes 2007 Feb;21(1):47-55.
          doi: 10.1016/j.mcp.2006.08.001pubmed: 17014984google scholar: lookup
        16. Koo H, Ryu SH, Ahn HJ, Jung WK, Park YK, Kwon NH, Kim SH, Kim JM, Yoo BW, Choi SI, Davis WC, Park YH. Immunostimulatory effects of the anionic alkali mineral complex Barodon on equine lymphocytes. Clin Vaccine Immunol 2006 Nov;13(11):1255-66.
          doi: 10.1128/CVI.00150-06pubmed: 16943344google scholar: lookup
        17. Vahlenkamp TW, Konrath A, Weber M, Müller H. Persistence of Borna disease virus in naturally infected sheep. J Virol 2002 Oct;76(19):9735-43.
          doi: 10.1128/jvi.76.19.9735-9743.2002pubmed: 12208952google scholar: lookup
        18. Hausmann J, Schamel K, Staeheli P. CD8(+) T lymphocytes mediate Borna disease virus-induced immunopathology independently of perforin. J Virol 2001 Nov;75(21):10460-6.
          doi: 10.1128/JVI.75.21.10460-10466.2001pubmed: 11581414google scholar: lookup
        19. Schamel K, Staeheli P, Hausmann J. Identification of the immunodominant H-2K(k)-restricted cytotoxic T-cell epitope in the Borna disease virus nucleoprotein. J Virol 2001 Sep;75(18):8579-88.
          doi: 10.1128/jvi.75.18.8579-8588.2001pubmed: 11507203google scholar: lookup
        20. Jordan I, Lipkin WI. Borna disease virus. Rev Med Virol 2001 Jan-Feb;11(1):37-57.
          doi: 10.1002/rmv.300pubmed: 11241801google scholar: lookup
        21. Fukuda K, Takahashi K, Iwata Y, Mori N, Gonda K, Ogawa T, Osonoe K, Sato M, Ogata S, Horimoto T, Sawada T, Tashiro M, Yamaguchi K, Niwa S, Shigeta S. Immunological and PCR analyses for Borna disease virus in psychiatric patients and blood donors in Japan. J Clin Microbiol 2001 Feb;39(2):419-29.
          doi: 10.1128/JCM.39.2.419-429.2001pubmed: 11158085google scholar: lookup
        22. Degiorgis MP, Berg AL, Hârd Af Segerstad C, Mörner T, Johansson M, Berg M. Borna disease in a free-ranging lynx (Lynx lynx). J Clin Microbiol 2000 Aug;38(8):3087-91.
          doi: 10.1128/JCM.38.8.3087-3091.2000pubmed: 10921984google scholar: lookup
        23. Hausmann J, Hallensleben W, de la Torre JC, Pagenstecher A, Zimmermann C, Pircher H, Staeheli P. T cell ignorance in mice to Borna disease virus can be overcome by peripheral expression of the viral nucleoprotein. Proc Natl Acad Sci U S A 1999 Aug 17;96(17):9769-74.
          doi: 10.1073/pnas.96.17.9769pubmed: 10449769google scholar: lookup
        24. Nöske K, Bilzer T, Planz O, Stitz L. Virus-specific CD4+ T cells eliminate borna disease virus from the brain via induction of cytotoxic CD8+ T cells. J Virol 1998 May;72(5):4387-95.
          doi: 10.1128/JVI.72.5.4387-4395.1998pubmed: 9557729google scholar: lookup
        25. Gonzalez-Dunia D, Sauder C, de la Torre JC. Borna disease virus and the brain. Brain Res Bull 1997;44(6):647-64.
          doi: 10.1016/s0361-9230(97)00276-1pubmed: 9421127google scholar: lookup
        26. Sobbe M, Bilzer T, Gommel S, Nöske K, Planz O, Stitz L. Induction of degenerative brain lesions after adoptive transfer of brain lymphocytes from Borna disease virus-infected rats: presence of CD8+ T cells and perforin mRNA. J Virol 1997 Mar;71(3):2400-7.
          doi: 10.1128/JVI.71.3.2400-2407.1997pubmed: 9032377google scholar: lookup
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