Presence of natural anti-Galalpha1-4GalNAcbeta1-3Gal (anti-NOR) antibodies in animal sera.
Abstract: Rare polyagglutinable NOR erythrocytes contain unusual globoside extention products terminating with a Galalpha1-4GalNAcbeta1-3Gal- unit. This trisaccharide epitope is recognized by recently characterized antibodies naturally occurring in most human sera (Duk et al., Glycobiology, 15, 109, 2005). These antibodies represent two major types of fine specificity. All these antibodies are most strongly inhibited by Galalpha1-4GalNAcbeta1-3Gal (NOR-tri), and weakly by Galalpha1-4Gal. However, the type 1 antibodies are strongly inhibited by Galalpha1-4Galbeta1-3Gal-R and weakly by Galalpha1-4GalNAc, while the type 2 antibodies show the opposite reactivities with these two oligosaccharides. Similar antibodies have now been found in horse, rabbit and pig sera. The antibodies were purified from animal sera by affinity chromatography on Galalpha1-4GalNAcbeta1-3Gal-human serum albumin(HSA)-Sepharose 4B conjugate. The specificity of the antibodies was determined by binding to ELISA plates coated with several alpha-galactosylated oligosaccharide-polyacrylamide (PAA) or -HSA conjugates and by inhibition with synthetic oligosaccharides. The purified antibodies bound specifically to conjugates containing NOR-tri. The inhibition of binding showed that the animal sera also contain two types of anti-NOR antibodies: type 2 was found in the horse serum, and a mixture of both types was present in rabbit and pig serum. These results indicate that anti-NOR, a new and distinct kind of anti-alphaGal antibody, are present in animal sera and show similar specificties and diversity as their counterparts found in human sera.
Publication Date: 2006-09-29 PubMed ID: 17006649DOI: 10.1007/s10719-006-8188-8Google Scholar: Lookup
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- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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This study investigates the presence of anti-Galalpha1-4GalNAcbeta1-3Gal (anti-NOR) antibodies in animal blood, which have previously been found in most human blood samples. The findings suggest that these specific antibodies also exist in animal blood, with similar characteristics as those in human blood.
Understanding Anti-NOR Antibodies
This research is centered around the study of anti-Galalpha1-4GalNAcbeta1-3Gal (anti-NOR) antibodies. These antibodies interact with a specific trisaccharide epitope found on NOR erythrocytes, a rare type of red blood cell. This specific interaction was only recently discovered and found to naturally occur in most human blood samples.
- Type 1 antibodies are inhibited strongly by Galalpha1-4Galbeta1-3Gal-R and weakly by Galalpha1-4GalNAc.
- Type 2 antibodies display the opposite reaction, being weakly inhibited by Galalpha1-4Galbeta1-3Gal-R and strongly by Galalpha1-4GalNAc.
Presence of Anti-NOR Antibodies in Animals
This paper reveals that anti-NOR antibodies are not exclusive to humans; they are also found in horse, rabbit, and pig blood. These animal’s sera samples were tested for these antibodies using a technique known as affinity chromatography.
- Using this technique, the research found that horse serum contained type 2 anti-NOR antibodies.
- Rabbit and pig serum presented a mixture of both type 1 and type 2 antibodies.
Research Implications and Conclusions
The detection of these antibodies in various animal sera indicates that anti-NOR antibodies are not unique to human sera. Moreover, the antibody types and their respective inhibitions seem to be conserved across the different species. These antibodies, figuring under a new and distinct category of anti-alphaGal antibodies, could potentially broaden our understanding of immune responses across species. Further research is required to determine the precise role and impact of these antibodies on animal health and disease.
Cite This Article
APA
Duk M, Lisowska E.
(2006).
Presence of natural anti-Galalpha1-4GalNAcbeta1-3Gal (anti-NOR) antibodies in animal sera.
Glycoconj J, 23(7-8), 585-590.
https://doi.org/10.1007/s10719-006-8188-8 Publication
Researcher Affiliations
- Department of Immunochemistry, Ludwik Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Rudolf Weigl St. 12, 53-114, Wroclaw, Poland.
MeSH Terms
- ABO Blood-Group System / immunology
- Animals
- Antibodies / blood
- Antibody Specificity
- Erythrocytes / immunology
- Globosides / immunology
- Horses
- Humans
- Immunity, Innate
- Oligosaccharides / pharmacology
- Rabbits
- Species Specificity
- Sus scrofa
- Trisaccharides / immunology
References
This article includes 14 references
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Citations
This article has been cited 2 times.- Doherty MK, Shaw C, Woods L, Weimer BC. Alpha-Gal Bound Aptamer and Vancomycin Synergistically Reduce Staphylococcus aureus Infection In Vivo. Microorganisms 2023 Jul 8;11(7).
- Bereznicka A, Mikolajczyk K, Szymczak-Kulus K, Kapczynska K, Majorczyk E, Modlinska A, Piasecki T, Kaczmarek R, Czerwinski M. Two Paralogous Gb3/CD77 Synthases in Birds Show Different Preferences for Their Glycoprotein and Glycosphingolipid Substrates. Int J Mol Sci 2021 Sep 9;22(18).
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