Preservation of differential staining of spermatozoa by formol citrate.

This research article examines the use of formol citrate and formol saline in the preservation of sperm samples from various species, and their effect on the staining properties of the sperm cells.

Sample Collection and Treatment

• Samples were collected from various animals including boars, bulls, rams, rabbits, reindeer, and stallions.
• These sperm samples were then diluted in either formol citrate or formol saline.
• The treated sperm samples were then dyed using eosinnigrosin, a dye that stains eosinophilic spermatozoa.

Storage Conditions

• Post-treatment, the samples were stored for a period of 48 hours under a variety of temperature conditions, ranging from 4 degrees Celsius to 40 degrees Celsius.
• A subset of the samples was kept for extended periods of up to 3 weeks to observe changes over a longer term.

Observation and Findings

• The proportion of eosinophilic spermatozoa, referring to sperm cells that took up the eosin nigrasin stain, did not change significantly compared to fresh semen samples after storage for 48 hours in the formol diluent.
• In regards to the samples stored for up to 3 weeks, the increase in the proportion of eosinophilic spermatozoa was found to be minimal.
• This indicates that formol citrate and formol saline are effective in preserving the staining properties of the sperm cells, even under a wide range of temperature conditions and extended storage durations.

From this study, it can be concluded that formol-based diluents can be used successfully for the staining and long-term storage of spermatozoa across a diverse range of animal species.