Preservation of viable Taylorella equigenitalis in different commercially available transport systems.
Abstract: The cultural diagnosis of the causal agent of contagious equine metritis (Taylorella equigenitalis) using transport swabs is challenging. Swabs must be placed in Amies charcoal medium, refrigerated during transport, and plated out at the laboratory no later than 48 h after sampling. In this study, the viability of T. equigenitalis strain CIP 79.7T in 11 commercial swab transport systems was initially compared at 1 day and 2 days of storage at ambient (20 ± 3 °C) or refrigerated (5 ± 3 °C) temperature. The four best swab transport systems, systems B, E, F (used as the reference) and K, were then compared at 0, 2, 3, 4, 7 and 10 days at refrigerated temperatures. Statistically significant differences were observed after 10 days only for system K compared to the reference, with approximately 95% viable T. equigenitalis recovered in system K compared to approximately 77% in system F. System K is thus promising for preservation and transport of viable T. equigenitalis for culture.
Copyright © 2021 Elsevier Ltd. All rights reserved.
Publication Date: 2021-02-08 PubMed ID: 33641806DOI: 10.1016/j.tvjl.2021.105629Google Scholar: Lookup
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- Comparative Study
- Journal Article
- Cell Viability
- Clinical Study
- Comparative Study
- Contagious Equine Metritis
- Diagnosis
- Diagnostic Technique
- Disease control
- Disease Diagnosis
- Disease Treatment
- Equine Diseases
- Equine Health
- In Vitro Research
- Infection
- Laboratory Methods
- Refrigeration
- Taylorella equigenitalis
- Temperature
- Veterinary Care
- Veterinary Medicine
- Veterinary Research
Summary
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This study focuses on how well different commercial transport swabs can maintain the viability of Taylorella equigenitalis, a bacteria causing contagious equine metritis in horses, under varying conditions for potential laboratory analysis.
About the Study
- The research was prompted by the challenge faced in transporting swabs used in the diagnosis of contagious equine metritis caused by Taylorella equigenitalis.
- The swabs need specific handling, including placement in Amies charcoal medium, refrigerating during transport and arrival at the lab within 48 hours of collection.
- The scientists investigated the performance of 11 different commercial swab transport systems to preserve T. equigenitalis at one and two days of storage either at room temperature or under refrigerated conditions.
Study Findings
- The study found that four transport systems, B, E, F (which was used as the reference) and K, were most effective.
- The four systems were further compared over different durations (0, 2, 3, 4, 7 and 10 days) under refrigeration.
- Among all, system K showed a significant statistical advantage after 10 days compared to the reference system where approximately 95% of T. equigenitalis remained viable in system K versus around 77% in system F.
Final Conclusion
- The study concluded that system K is particularly promising for preserving and transporting viable T. equigenitalis for laboratory culture.
This means it could potentially be more effective in the field, leading to better diagnosis and control of contagious equine metritis. Further studies may still be needed to confirm its effectiveness under various field conditions.
Cite This Article
APA
Duquesne F, Breuil MF, Hans A, Petry S.
(2021).
Preservation of viable Taylorella equigenitalis in different commercially available transport systems.
Vet J, 270, 105629.
https://doi.org/10.1016/j.tvjl.2021.105629 Publication
Researcher Affiliations
- ANSES, Laboratory for Animal Health, Physiopathology and Epidemiology of Equine Diseases Unit, Goustranville, France.
- ANSES, Laboratory for Animal Health, Physiopathology and Epidemiology of Equine Diseases Unit, Goustranville, France.
- ANSES, Laboratory for Animal Health, Physiopathology and Epidemiology of Equine Diseases Unit, Goustranville, France.
- ANSES, Laboratory for Animal Health, Physiopathology and Epidemiology of Equine Diseases Unit, Goustranville, France. Electronic address: sandrine.petry@anses.fr.
MeSH Terms
- Animals
- Endometritis / microbiology
- Endometritis / veterinary
- Female
- Gram-Negative Bacterial Infections / microbiology
- Gram-Negative Bacterial Infections / veterinary
- Horse Diseases / microbiology
- Horses
- Preservation, Biological / instrumentation
- Preservation, Biological / methods
- Preservation, Biological / veterinary
- Refrigeration
- Sexually Transmitted Diseases, Bacterial / microbiology
- Sexually Transmitted Diseases, Bacterial / veterinary
- Specimen Handling / instrumentation
- Specimen Handling / methods
- Specimen Handling / veterinary
- Taylorella equigenitalis / physiology
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