Prevalence of equine herpesvirus types 2 and 5 in horse populations by using type-specific PCR assays.
Abstract: Equineherpesvirustypes 2 and 5 (EHV-2andEHV-5)have a rather unclearpathogenicity and distribution within the equid population. In order to gain more information on the prevalence of these two viruses, type-specific PCR assays were developed to detect viral DNA in nasal specimens and in peripheral blood leukocytes (PBLs) of adult horses and foals from various regions of Europe, i.e. Sweden, Hungary and the United Kingdom. In adult horses, the prevalence of EHV-2 in PBLs was up to 68% in Sweden and 71% in the United Kingdom. EHV-2 DNA was detected in the PBLs from all the foals tested in all countries and most (93%) of the nasal specimens also yielded positive results. The prevalence of EHV-5 DNA in the PBLs of foals in Hungary was 15 and 24% in adult horses in the United Kingdom. This observation was among the very few reports of the presence of EHV-5 in horses. In summary, the specific PCR assays revealed important data on the occurrence and distribution of EHV-2 and EHV-5 in large horse populations. The findings indicated that infection with EHV-5 occurred later than EHV-2 in foals. This study may contribute to a better understanding of the etiological role of these gammaherpesviruses in equine diseases.
Publication Date: 2002-06-12 PubMed ID: 12056476DOI: 10.1051/vetres:2002013Google Scholar: Lookup
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- Journal Article
Summary
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This research explored the presence of equine herpesvirus types 2 and 5 in various horse populations across Europe using type-specific PCR tests, to help understand the impact and distribution of these viruses.
Understanding the Study
- The research focused on equine herpesvirus types 2 and 5 (EHV-2 and EHV-5), which are diseases affecting horses but not completely understood in terms of their pathogenesis and spread within horse populations.
- To get a clearer idea of the prevalence of these two viruses, the researchers employed type-specific Polymerase Chain Reaction (PCR) tests. These tests can detect the viruses’ genetic materials in nasal specimens and peripheral blood leukocytes (PBLs), a type of white blood cell, from adult horses and foals.
- The study included equines from different parts of Europe, namely Sweden, Hungary, and the United Kingdom.
Findings of the Study
- In adult horses, EHV-2 was found in up to 68% of those in Sweden and 71% in the United Kingdom, according to the PBLs samples.
- All the foals tested in the study were found to have EHV-2, and 93% of the nasal samples from these young horses also had the virus. This suggests that EHV-2 is widely prevalent among both adult horses and foals.
- The prevalence of EHV-5 was much lower compared to EHV-2. The virus was found in 15% of foals in Hungary and in 24% of adult horses in the United Kingdom. There have been few reports of EHV-5 in horses, making this a significant observation.
Implications of the Research
- This research has provided valuable data on the presence and spread of EHV-2 and EHV-5 in large horse populations. Such information can help in developing strategies to manage and control these viruses among equids.
- The study also indicates that EHV-5 infections tend to occur later than EHV-2 in foals. This newfound knowledge could provide insight into the progression and development of these diseases in horses, leading to more effective treatments and interventions.
- Overall, a better understanding of these gammaherpesviruses can lead to the development of preventative measures and treatments for these debilitating equine diseases.
Cite This Article
APA
Nordengrahn A, Merza M, Ros C, Lindholmc A, Palfl V, Hannant D, Belák S.
(2002).
Prevalence of equine herpesvirus types 2 and 5 in horse populations by using type-specific PCR assays.
Vet Res, 33(3), 251-259.
https://doi.org/10.1051/vetres:2002013 Publication
Researcher Affiliations
- Svanova Biotech AB, Uppsala Science Park, Glunten, Sweden. ann.nordengrahn@svanova.com
MeSH Terms
- Age Factors
- Animals
- DNA, Viral / analysis
- Female
- Gammaherpesvirinae / genetics
- Gammaherpesvirinae / isolation & purification
- Herpesviridae
- Herpesviridae Infections / blood
- Herpesviridae Infections / epidemiology
- Herpesviridae Infections / veterinary
- Horse Diseases / blood
- Horse Diseases / epidemiology
- Horse Diseases / virology
- Horses
- Hungary / epidemiology
- Male
- Polymerase Chain Reaction / veterinary
- Prevalence
- Rhadinovirus / genetics
- Rhadinovirus / isolation & purification
- Sensitivity and Specificity
- Seroepidemiologic Studies
- Sweden / epidemiology
- Tumor Virus Infections / blood
- Tumor Virus Infections / epidemiology
- Tumor Virus Infections / veterinary
- United Kingdom / epidemiology
Citations
This article has been cited 18 times.- Badr C, Souiai O, Arbi M, El Behi I, Essaied MS, Khosrof I, Benkahla A, Chabchoub A, Ghram A. Epidemiological and Phylogeographic Study of Equid Herpesviruses in Tunisia.. Pathogens 2022 Sep 5;11(9).
- Wondimagegnehu K, Leta S, Amenu K, Negussie H. Molecular detection and assessment of the epidemiological risk factors associated with equine herpesvirus 2 and 5 in working equids in central Ethiopia.. Vet Med Sci 2022 Nov;8(6):2396-2403.
- Onasanya AE, El-Hage C, Diaz-Méndez A, Vaz PK, Legione AR, Browning GF, Devlin JM, Hartley CA. Whole genome sequence analysis of equid gammaherpesvirus -2 field isolates reveals high levels of genomic diversity and recombination.. BMC Genomics 2022 Aug 30;23(1):622.
- Stasiak K, Dunowska M, Rola J. Kinetics of the Equid Herpesvirus 2 and 5 Infections among Mares and Foals from Three Polish National Studs.. Viruses 2022 Mar 29;14(4).
- El-Hage C, Mekuria Z, Dynon K, Hartley C, McBride K, Gilkerson J. Association of Equine Herpesvirus 5 with Mild Respiratory Disease in a Survey of EHV1, -2, -4 and -5 in 407 Australian Horses.. Animals (Basel) 2021 Nov 30;11(12).
- Scheurer L, Bachofen C, Hardmeier I, Lechmann J, Schoster A. Prevalence of Nasal Shedding of Equid Gammaherpesviruses in Healthy Swiss Horses.. Viruses 2021 Aug 25;13(9).
- Partin TG, Schrenzel MD, Braun J, Witte CL, Kubiski SV, Lee J, Rideout BA. Herpesvirus surveillance and discovery in zoo-housed ruminants.. PLoS One 2021;16(1):e0246162.
- Anis E, Ilha MRS, Engiles JB, Wilkes RP. Evaluation of targeted next-generation sequencing for detection of equine pathogens in clinical samples.. J Vet Diagn Invest 2021 Mar;33(2):227-234.
- Seo MG, Ouh IO, Lee SK, Lee JS, Kwon OD, Kwak D. Molecular Detection and Genetic Characteristics of Equine Herpesvirus in Korea.. Pathogens 2020 Feb 11;9(2).
- Thorsteinsdóttir L, Jónsdóttir S, Stefánsdóttir SB, Andrésdóttir V, Wagner B, Marti E, Torsteinsdóttir S, Svansson V. The effect of maternal immunity on the equine gammaherpesvirus type 2 and 5 viral load and antibody response.. PLoS One 2019;14(6):e0218576.
- Dall Agnol AM, Beuttemmuller EA, Pilz D, Leme RA, Saporiti V, Headley SA, Alfieri AF, Alfieri AA. Detection of Equid gammaherpesvirus 2 and 5 DNA in the upper respiratory tract of asymptomatic horses from Southern Brazil.. Braz J Microbiol 2019 Jul;50(3):875-878.
- Marenzoni ML, Stefanetti V, Danzetta ML, Timoney PJ. Gammaherpesvirus infections in equids: a review.. Vet Med (Auckl) 2015;6:91-101.
- Stasiak K, Dunowska M, Rola J. Prevalence and sequence analysis of equid herpesviruses from the respiratory tract of Polish horses.. Virol J 2018 Jul 11;15(1):106.
- Williams KJ, Robinson NE, Lim A, Brandenberger C, Maes R, Behan A, Bolin SR. Experimental induction of pulmonary fibrosis in horses with the gammaherpesvirus equine herpesvirus 5.. PLoS One 2013;8(10):e77754.
- Marenzoni ML, Bietta A, Lepri E, Casagrande Proietti P, Cordioli P, Canelli E, Stefanetti V, Coletti M, Timoney PJ, Passamonti F. Role of equine herpesviruses as co-infecting agents in cases of abortion, placental disease and neonatal foal mortality.. Vet Res Commun 2013 Dec;37(4):311-7.
- Back H, Kendall A, Grandón R, Ullman K, Treiberg-Berndtsson L, Ståhl K, Pringle J. Equine multinodular pulmonary fibrosis in association with asinine herpesvirus type 5 and equine herpesvirus type 5: a case report.. Acta Vet Scand 2012 Sep 25;54(1):57.
- Marenzoni ML, Coppola G, Maranesi M, Passamonti F, Cappelli K, Capomaccio S, Verini Supplizi A, Thiry E, Coletti M. Age-dependent prevalence of equid herpesvirus 5 infection.. Vet Res Commun 2010 Dec;34(8):703-8.
- Burnouf T, Griffiths E, Padilla A, Seddik S, Stephano MA, Gutiérrez JM. Assessment of the viral safety of antivenoms fractionated from equine plasma.. Biologicals 2004 Sep;32(3):115-28.
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