Prevalence of Taylorella equigenitalis in Icelandic mares and geldings in Southern Germany and Austria.
Abstract: Contagious Equine Metritis (CEM) caused by the bacterium Taylorella equigenitalis (T. equigenitalis), is a venereal infection of equids which is of international concern to the equine breeding industry. A recent study showed a high prevalence of T. equigenitalis in Icelandic stallions when compared to stallions of other breeds also using for natural breeding. Consequently, the objectives of the present study were to investigate the prevalence of T. equigenitalis in Icelandic mares and geldings and to determine factors associated with a T. equigenitalis-positive qPCR result. In total, 361 Icelandic horses located in Southern Germany and Austria were tested for T. equigenitalis using a qPCR assay. An overall prevalence of 14.4 % was detected. Positive qPCR results were found in 2.2 % (3/134) of brood mares, 9.0 % (11/122) of maiden mares and in 36.2 % (38/105) of geldings. The odds for a T. equigenitalis-positive qPCR result were significantly lower in both brood (OR = 40.1, 95 % CI: 8.38-192, P < 0.001) and maiden mares (OR = 9.51, 95 % CI: 3.26-25.7, P < 0.001) when compared to geldings. Advancing age was not associated with higher odds for a T. equigenitalis-positive qPCR result (OR = 0.98, 95 % CI: 0.94-1.03, P = 0.51). However, horses of the younger age group showed significantly lower Ct values compared to horses of the older age group (P = 0.04). Furthermore, geldings showed significantly lower Ct values than brood (P < 0.03) and maiden mares (P < 0.001). This study showed a significantly higher prevalence of T. equigenitalis in Icelandic geldings compared to Icelandic mares. Icelandic geldings might therefore represent a reservoir for T. equigenitalis.
Copyright © 2024 The Author(s). Published by Elsevier Inc. All rights reserved.
Publication Date: 2024-11-30 PubMed ID: 39622457DOI: 10.1016/j.jevs.2024.105247Google Scholar: Lookup
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- Journal Article
Summary
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The research study focuses on the prevalence of a bacterium, Taylorella equigenitalis, which causes Contagious Equine Metritis in Icelandic mares and geldings found in Southern Germany and Austria. The study found a higher prevalence of the infection in Icelandic geldings as compared to mares.
Objective of the Study
- The study was conducted to primarily determine the prevalence of the infectious bacterium, Taylorella equigenitalis, in Icelandic mares and geldings, which are horses that have been neutered (castrated).
- Additionally, the study aimed to establish any associated factors that might increase the chances of a T. equigenitalis-positive qPCR (quantitative polymerase chain reaction) result.
Methodology
- A total of 361 Icelandic horses in Southern Germany and Austria were tested for the presence of T. equigenitalis using a qPCR assay, a laboratory technique used to amplify and simultaneously quantify a targeted DNA molecule.
Findings of the Study
- The study found an overall prevalence of T. equigenitalis in the population of horses to be 14.4%.
- Specific findings indicate that the bacterium was found in 2.2% of breeding mares, 9.0% of maiden mares (female horses that have never been used for breeding), and 36.2% of geldings.
- The results showed that when compared to geldings, both brood and maiden mares had significantly lower chances of testing positive for T. equigenitalis.
- The age of the horse did not show any significant correlation with higher odds of a T. equigenitalis-positive qPCR result.
- Horses in the younger age group showed significantly lower C values (cycle threshold values – used in qPCR to quantify the amount of target DNA) as compared to horses in the older age group.
- Geldings showed significantly lower C values than both types of mares.
Conclusion
- The study concluded by highlighting that there is a significantly higher prevalence of T. equigenitalis in Icelandic geldings compared to Icelandic mares.
- It is suggested that Icelandic geldings could potentially serve as a reservoir for the transmission and spread of T. equigenitalis, which is a cause of international concern to the horse breeding industry due to its contagious nature.
Cite This Article
APA
Solbach V, Grabatin M, Zablotski Y, Fux R, Zerbe H, Witte TS.
(2024).
Prevalence of Taylorella equigenitalis in Icelandic mares and geldings in Southern Germany and Austria.
J Equine Vet Sci, 144, 105247.
https://doi.org/10.1016/j.jevs.2024.105247 Publication
Researcher Affiliations
- Equine Clinic, Center for Clinical Veterinary Medicine, Faculty of Veterinary Medicine, Ludwig-Maximilians-Universität in Munich, Sonnenstrasse 14, 85764 Oberschleissheim, Germany.
- Equine Clinic, Center for Clinical Veterinary Medicine, Faculty of Veterinary Medicine, Ludwig-Maximilians-Universität in Munich, Sonnenstrasse 14, 85764 Oberschleissheim, Germany.
- Equine Clinic, Center for Clinical Veterinary Medicine, Faculty of Veterinary Medicine, Ludwig-Maximilians-Universität in Munich, Sonnenstrasse 14, 85764 Oberschleissheim, Germany.
- Division of Virology, Department of Veterinary Sciences, Ludwig-Maximilians-Universität in Munich, Sonnenstrasse 24, 85764 Oberschleissheim, Germany.
- Clinic for Ruminants with Ambulatory and Herd Health Services, Ludwig-Maximilians-Universität in Munich, Sonnenstrasse 16, 85764 Oberschleissheim, Germany.
- Equine Clinic, Center for Clinical Veterinary Medicine, Faculty of Veterinary Medicine, Ludwig-Maximilians-Universität in Munich, Sonnenstrasse 14, 85764 Oberschleissheim, Germany. Electronic address: T.Witte@lmu.de.
MeSH Terms
- Animals
- Horses
- Horse Diseases / epidemiology
- Horse Diseases / microbiology
- Female
- Austria / epidemiology
- Germany / epidemiology
- Taylorella equigenitalis / isolation & purification
- Prevalence
- Gram-Negative Bacterial Infections / veterinary
- Gram-Negative Bacterial Infections / epidemiology
- Gram-Negative Bacterial Infections / microbiology
- Male
Conflict of Interest Statement
Declaration of competing interest The authors declare no competing interests related to this report.
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