Probenecid infusion in mares: effect on para-aminohippuric acid clearance.

The research in this article evaluates the effect of probenecid infusion on the clearance of para-aminohippuric acid (PAHA) in mares.

Research Methodology

• The study was conducted by injecting para-aminohippuric acid (PAHA), at a rate of 0.1 mg per minute for each kilogram of body weight, intravenously into two mares.
• Following this, a simultaneous intravenous infusion of PAHA and probenecid, at varying substance levels (0.075, 0.15, 0.25, or 0.35 mg of probenecid per minute for each kilogram of body weight), was administered.

Findings: Effect on Clearance of PAHA

• The infusion of probenecid led to a reduction in the clearance of PAHA when serum probenecid concentrations exceeded 55 micrograms per ml.
• All doses of probenecid resulted in a mean serum probenecid concentration greater than 55 micrograms per ml.

Findings: Probenecid Concentration at Different Dosages

• Dosages less than 200 mg/kg resulted in minimal accumulation of probenecid in serum from the 1st to the 5th dose.
• In contrast, when administered at a dosage of 200 mg per kg, probenecid began to accumulate in serum from the first to the fifth dose.

Findings: Effect of Multiple Doses of Probenecid

• When mares were subjected to intragastric administration of probenecid (at 75 mg per kg) five times at 12-hour intervals, it reduced the clearance of PAHA by 50%.

Findings: Bioavailability of Probenecid

• The research shows that the bioavailability of probenecid was 117% and 102% for the two mares, respectively, after a single intragastric dose. This was in comparison with a single intravenous dose.

Implications

• The research is significant as it suggests that the clearance of PAHA in mares can be controlled by manipulating the dosage and frequency of probenecid administration.