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Microbial pathogenesis2021; 165; 105216; doi: 10.1016/j.micpath.2021.105216

Probiotic potential of Lactobacillus isolated from horses and its therapeutic effect on DSS-induced colitis in mice.

Abstract: Inflammatory bowel disease (IBD) is a refractory disease that endangers both humans and animals. In recent times, Lactobacillus have been used to treat animal diseases. It may be a good choice to try to isolate Lactobacillus with probiotic potential to treat IBD. Equine, as a kind of hindgut fermentation animal has rich intestinal microflora, but data regarding this is scarce. The isolation of Lactobacillus with probiotic potential from equine may become a new method for the treatment of IBD. Four isolates of Lactobacillus were isolated from fresh feces of healthy male adult horses and analyzed their biological characteristics. According to the phylogenetic analysis, A2.5 and A7.1 were identified as Pediococcus pentosaceus, A3 as Lactobacillus plantarum, and B8.2 as Weissella cibaria. All four isolates showed tolerance to the environment of acid, bile salt concentration and simulated artificial gastrointestinal fluid. The hydrophobic rate and self-aggregation rate of A3 were close to 100%, and the adhesion rate was 28.85 ± 0.74%. Four isolates were negative in hemolysis test and sensitive to common antibiotics and different isolates had different sensitivity to antibiotics. The four isolates had antibacterial and antioxidant activities which can reflect their probiotic potential. Furthermore, they could regulate the LPS (Lipopolysaccharides) stimulated Caco-2 cells. We chose A3 as the treatment strain to intervene Dextran sulfate sodium salt (DSS)-induced mice. The results showed that compared with DSS group, DSS + A3 group exhibited reduced Disease activity index (DAI), increased colon length, reduced pathological score and regulated cytokine secretion at the level of gene expression. In this study, four isolates of Lactobacillus with probiotic potential were isolated, and Lactobacillus plantarum A3 with reduced ulcerative colitis in mice was screened. It might provide a potential treatment for IBD.
Copyright © 2021 Elsevier Ltd. All rights reserved.
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Publication Date: 2021-09-29 PubMed ID: 34600098DOI: 10.1016/j.micpath.2021.105216Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research aims to discover the potential therapeutic benefits of Lactobacillus, particularly its strain Lactobacillus plantarum A3, isolated from horses on Inflammatory Bowel Disease (IBD) observed in a Dextran sulfate sodium salt induced colitis mouse model.

Study Objectives and Background

  • The research stemmed from the observation that IBD is a challenging disease affecting both humans and animals, often resistant to treatment.
  • Lactobacillus, `a bacteria with possible probiotic characteristics, has been utilized to treat various diseases in animals.
  • The study aimed to explore this bacteria, specifically strains of Lactobacillus isolated from horses, to evaluate its effects on an IBD model in mice.
  • Horses, as hindgut fermentation animals, have an abundance of gut microflora, including various strains of Lactobacillus. However, detailed data on these microbes and their potential uses are limited.

Methods and Results

  • The researchers isolated four isolates of Lactobacillus from the feces of healthy adult male horses and analyzed their biological characteristics.
  • The resultant isolates named A2.5, A7.1, A3 and B8.2 were identified as Pediococcus pentosaceus, Lactobacillus plantarum, and Weissella cibaria respectively.
  • It was documented that all four isolates demonstrated tolerance to acidic environments, bile salt concentrations and artificial gastrointestinal fluid, indicating potential viability in the harsh conditions of the gut.
  • Further evaluations showed Lactobacillus plantarum A3 (specified as A3 in the paper) displayed a high self-aggregation rate and hydrophobicity, along with an adhesion rate of approximately 28.85%.
  • None of the isolates were hemolytic, and they showed sensitivity to commonly used antibiotics.
  • The bacteria isolates also showed promising antibacterial and antioxidant activities. Furthermore, these isolates were found to regulate lipid A-like molecules, lipopolysaccharides (LPS) -stimulated Caco-2 cells which are common model cells for research on the intestine.
  • After this evaluation, Lactobacillus plantarum A3 was selected for further studies. The researchers used this strain to treat mice induced with colitis through the administration of Dextran sulfate sodium salt (DSS).
  • Compared to the control group, mice treated by Lactobacillus plantarum A3 showed significant improvements in their Disease activity index (DAI), colon length, and pathological scores. They also exhibited a regulated cytokine secretion at the gene expression level, thus indicating reduced inflammation.

Conclusions and Implications

  • This research successfully isolated and identified potentially beneficial strains of Lactobacillus from the feces of healthy horses.
  • Among these, Lactobacillus plantarum A3 showed promising effects on IBD in a mouse model.
  • This study thus provides early evidence that Lactobacillus plantarum A3 might be used as a potential treatment for IBD in the future. However, further studies in more complex models will be needed to confirm these results.

Cite This Article

APA
Qin S, Huang Z, Wang Y, Pei L, Shen Y. (2021). Probiotic potential of Lactobacillus isolated from horses and its therapeutic effect on DSS-induced colitis in mice. Microb Pathog, 165, 105216. https://doi.org/10.1016/j.micpath.2021.105216

Publication

Microbial pathogenesis
ISSN: 1096-1208
NlmUniqueID: 8606191
Country: England
Language: English
Volume: 165
Pages: 105216

Researcher Affiliations

Qin, Songkang
  • College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, 430070, China.
Huang, Zonghao
  • College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, 430070, China.
Wang, Yingli
  • College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, 430070, China.
Pei, Lulu
  • College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, 430070, China.
Shen, Yaoqin
  • College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, 430070, China. Electronic address: yshen@mail.hzau.edu.cn.

MeSH Terms

  • Animals
  • Anti-Bacterial Agents / therapeutic use
  • Caco-2 Cells
  • Colitis / chemically induced
  • Colitis / therapy
  • Dextran Sulfate / adverse effects
  • Disease Models, Animal
  • Horses
  • Humans
  • Inflammatory Bowel Diseases / drug therapy
  • Lactobacillus
  • Lactobacillus plantarum
  • Lipopolysaccharides / adverse effects
  • Male
  • Mice
  • Phylogeny
  • Probiotics / therapeutic use

Citations

This article has been cited 13 times.
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