Production of calcium maintenance factor Stanniocalcin-1 (STC1) by the equine endometrium during the early pregnant period.
Abstract: A factor responsible for progression to pregnancy establishment in the mare has not been definitively characterized. To identify factors possibly involved in the establishment of equine pregnancy, the endometrium was collected from day 13 (day 0=day of ovulation) cyclic and day 13, 19 and 25 pregnant animals. From initial subtractive hybridization studies, a calcium regulating factor, Stanniocalcin-1 (STC1) mRNA, was found as a candidate molecule expressed uniquely in the pregnant endometrium. Endometrial expression of STC1 mRNA was noted on day 19 and was markedly increased in the day 25 gravid endometrium. STC1 protein was found in the extracts of day 25 gravid endometrium and immunochemically localized in the uterine glands. In addition, STC1 protein was detected in uterine flushing media collected from day 25 pregnant mares. High concentrations of estradiol-17 β (E(2)) were detected in day 25 conceptuses. E(2) levels were much higher in the gravid endometrium than in other regions, whereas progesterone levels did not differ among the samples from different endometrial regions. Expression of STC1 mRNA, however, was not significantly upregulated in cultured endometrial explants treated with various concentrations of E(2) (0.01-100 ng/ml) with or without 10 ng/ml progesterone. These results indicate that an increase in STC1 expression appears to coincide with capsule disappearance in the conceptus, and suggest that STC1 from the uterine glands likely plays a role in conceptus development during the pregnancy establishment period in the mare.
Publication Date: 2010-12-03 PubMed ID: 21139325DOI: 10.1262/jrd.10-079kGoogle Scholar: Lookup
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- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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The research article delves into equine pregnancy by investigating factors contributing to its establishment. The study pays special attention to Stanniocalcin-1 (STC1), a calcium regulating factor present in the endometrium of pregnant equines.
Objective and Methodology
In an endeavor to identify factors contributing to the establishment of equine pregnancy, the researchers strategically collected endometrium samples from mares at fixed intervals – day 13 of the cycle (with day 0 being the day of ovulation), and on days 13, 19, and 25 of pregnancy.
Results
From the analysis, the following pivotal aspects surfaced:
- Stanniocalcin-1 (STC1), a calcium-regulating factor, emerged as a primary candidate, uniquely expressed in the pregnant endometrium.
- A noticeable expression of STC1 mRNA was documented on day 19 and saw a significant rise in the gravid endometrium on day 25.
- On day 25, STC1 protein was discovered in the extracts of the gravid endometrium and was immunochemically identified in the uterine glands. Furthermore, the same protein was found in the uterine flushing media collected on the same day.
- Exceptionally high concentrations of estradiol-17 β (E(2)) was detected in the conceptuses on day 25, which was notably higher in the gravid endometrium in contrast to other regions. However, progesterone levels remained consistently the same in samples collected from different regions of the endometrium.
- Despite the high E(2) concentration, the treatment of various E(2) levels, with or without progesterone, did not significantly upregulate the expression of STC1 mRNA in cultured endometrial explants.
Conclusion
The research study concluded that the increase in STC1 expression is synchronized with the disappearance of the capsule in the conceptus. It suggests that the STC1 from uterine glands probably contributes to the conceptus development, thereby playing a crucial role during pregnancy establishment in mares.
Cite This Article
APA
Kikuchi M, Nakano Y, Nambo Y, Haneda S, Matsui M, Miyake Y, Macleod JN, Nagaoka K, Imakawa K.
(2010).
Production of calcium maintenance factor Stanniocalcin-1 (STC1) by the equine endometrium during the early pregnant period.
J Reprod Dev, 57(2), 203-211.
https://doi.org/10.1262/jrd.10-079k Publication
Researcher Affiliations
- Faculty of Agriculture, The University of Tokyo, Tokyo, Japan.
MeSH Terms
- Animals
- Endometrium / metabolism
- Estradiol / metabolism
- Female
- Glycoproteins / metabolism
- Horses / metabolism
- Pregnancy / genetics
- Pregnancy / metabolism
- Progesterone / metabolism
- RNA, Messenger / metabolism
Citations
This article has been cited 10 times.- Liu J, Liu Y, Yang C, Liu J, Hao J. Comprehensive analysis for the immune related biomarkers of platinum-based chemotherapy in ovarian cancer.. Transl Oncol 2023 Aug 22;37:101762.
- Bishop A, Cartwright JE, Whitley GS. Stanniocalcin-1 in the female reproductive system and pregnancy.. Hum Reprod Update 2021 Oct 18;27(6):1098-1114.
- Luo W, Chen D, Wang H, Hu J. Stanniocalcin 1 is a prognostic biomarker in glioma.. Oncol Lett 2020 Sep;20(3):2248-2256.
- Zhao F, Yang G, Feng M, Cao Z, Liu Y, Qiu J, You L, Zheng L, Zhang T, Zhao Y. Expression, function and clinical application of stanniocalcin-1 in cancer.. J Cell Mol Med 2020 Jul;24(14):7686-7696.
- Khatun M, Arffman RK, Lavogina D, Kangasniemi M, Laru J, Ahtikoski A, Lehtonen S, Paulson M, Hirschberg AL, Salumets A, Andersson LC, Piltonen TT. Women with polycystic ovary syndrome present with altered endometrial expression of stanniocalcin-1†.. Biol Reprod 2020 Feb 14;102(2):306-315.
- Haneda S, Nagaoka K, Nambo Y, Kikuchi M, Nakano Y, Li J, Matsui M, Miyake YI, Imakawa K. Expression of uterine lipocalin 2 and its receptor during early- to mid-pregnancy period in mares.. J Reprod Dev 2017 Apr 21;63(2):127-133.
- Aghajanova L, Altmäe S, Kasvandik S, Salumets A, Stavreus-Evers A, Giudice LC. Stanniocalcin-1 expression in normal human endometrium and dysregulation in endometriosis.. Fertil Steril 2016 Sep 1;106(3):681-691.e1.
- Ma X, Gu L, Li H, Gao Y, Li X, Shen D, Gong H, Li S, Niu S, Zhang Y, Fan Y, Huang Q, Lyu X, Zhang X. Hypoxia-induced overexpression of stanniocalcin-1 is associated with the metastasis of early stage clear cell renal cell carcinoma.. J Transl Med 2015 Feb 12;13:56.
- Tachibana Y, Sakurai T, Bai H, Shiota K, Nambo Y, Nagaoka K, Imakawa K. RNA-seq analysis of equine conceptus transcripts during embryo fixation and capsule disappearance.. PLoS One 2014;9(12):e114414.
- Tachibana Y, Nakano Y, Nagaoka K, Kikuchi M, Nambo Y, Haneda S, Matsui M, Miyake Y, Imakawa K. Expression of endometrial immune-related genes possibly functioning during early pregnancy in the mare.. J Reprod Dev 2013;59(1):85-91.
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