Profiling of genetic markers useful for breeding decision in Selle Francais horse.
Abstract: Genetic disorders are recognised as hereditary diseases with the most significant economic impact on horse breeding, causing important foal losses, costs of treatments of horses, and maintenance of the mare during the pregnancy. The Selle Francais horses are recognized in many countries and are showing great results in equestrian sports around the world (dressage, show jumping and eventing). The study aimed to detect the presence of three mutant alleles associated with inherited diseases including Fragile Foal Syndrome (FFS), Cerebellar Abiotrophy (CA), Polysaccharide Storage Myopathy (PSSM1) and variant impacting gait type in DMRT3. This trait is important for breeding decision in Selle Francais horses and sheds new light on genetic potential and risks on this breed. The genotyping was performed on 91 Selle Francais horses using PCR-RFLP (for POLD1; GYS1 and DMRT3 genes) and PCR-ACRS (TOE1 gene) methods. The presented report indicated the presence of mutant allele A casual for PSSM1 and allele T associated with FFS syndrome occurrence, in 4% and 6% of analysed horses, respectively. Regarding CA, the present survey did not register any cases of this genetic disorder in Selle Francais horses. Our results show also that about 1% of all the Sell Francais horses studied carry the A allele of DMRT3 gene. The present findings have provided data for these fulness of monitoring genetic diseases and gait type in the investigated breed to avoid losses of offspring.
Copyright © 2022. Published by Elsevier Inc.
Publication Date: 2022-06-28 PubMed ID: 35777576DOI: 10.1016/j.jevs.2022.104059Google Scholar: Lookup
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- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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The study investigates the presence of specific genetic markers related to various diseases in the Selle Francais horse breed. The analysis conducted aims to help make more informed breeding decisions and minimize genetic disease-associated losses.
Objective of Research
The main aim was to detect the presence of three mutant alleles associated with inherited diseases including Fragile Foal Syndrome (FFS), Cerebellar Abiotrophy (CA), Polysaccharide Storage Myopathy (PSSM1) within the Selle Francais breed. Additionally, they sought to identify a variant that affects gait type in DMRT3, providing useful information for decision making in horse breeding.
Methodology
- The research team utilized PCR-RFLP, a form of genetic testing, on three genes (POLD1, GYS1, and DMRT3) to study their role in the targeted diseases.
- They used the PCR-ACRS method on the TOE1 gene to analyze its impact.
- These methods were performed on a sample of 91 Selle Francais horses.
Key Findings
- The researchers found that 4% of the tested horses carried the mutant allele, PSSM1, which is known to be associated with polysaccharide storage myopathy.
- 6% of the horses carried the allele T associated with Fragile Foal Syndrome.
- In terms of Cerebellar Abiotrophy (CA), the study did not register a single case among the tested sample.
- They discovered that about 1% of the Selle Francais horses carried the A allele of the gene DMRT3, which affects gait type.
Conclusion and Implications
- The findings of this research offer valuable data for breeders of the Selle Francais breed, allowing them to make more informed decisions and avoid potential offspring losses due to genetic disorders.
- The genotyping results may help in creating a specific breeding strategy considering the presence of mutant alleles that could lead to these diseases.
- Further, by understanding the distribution of these genetic traits, breeders can work towards optimizing breeding strategies to preserve the breed while minimizing the prevalence of these genetic diseases.
Cite This Article
APA
Ayad A, Besseboua O, Aissanou S, Stefaniuk-Szmukier M, Piórkowska K, Musiał AD, Długosz B, Kozłowska A, Ropka-Molik K.
(2022).
Profiling of genetic markers useful for breeding decision in Selle Francais horse.
J Equine Vet Sci, 116, 104059.
https://doi.org/10.1016/j.jevs.2022.104059 Publication
Researcher Affiliations
- Department of Environment and Biological Sciences, Faculty of Nature and Life Sciences, University of Bejaia, Bejaia 06000, Algeria.
- Department of Agronomic and Biotechnological Sciences, Faculty of Nature and Life Sciences, University H. Benbouali, Chlef 02000, Algeria.
- Department of Environment and Biological Sciences, Faculty of Nature and Life Sciences, University of Bejaia, Bejaia 06000, Algeria.
- Department of Animal Molecular Biology, National Research Institute of Animal Production, Krakowska 1, Balice 32-083, Poland.
- Department of Animal Molecular Biology, National Research Institute of Animal Production, Krakowska 1, Balice 32-083, Poland.
- Department of Animal Molecular Biology, National Research Institute of Animal Production, Krakowska 1, Balice 32-083, Poland.
- Department of Animal Reproduction, Anatomy and Genomics, University of Agriculture in Kraków, al. Mickiewicza 24/28, Kraków 30-059, Poland.
- Department of Animal Molecular Biology, National Research Institute of Animal Production, Krakowska 1, Balice 32-083, Poland.
- Department of Animal Molecular Biology, National Research Institute of Animal Production, Krakowska 1, Balice 32-083, Poland. Electronic address: katarzyna.ropka@iz.edu.pl.
MeSH Terms
- Alleles
- Animals
- Cerebellar Diseases / genetics
- Cerebellar Diseases / veterinary
- Female
- Gait / genetics
- Genetic Markers / genetics
- Horse Diseases / genetics
- Horses / genetics
- Muscular Diseases / genetics
- Muscular Diseases / veterinary
- Phenotype
- Polymorphism, Restriction Fragment Length
Conflict of Interest Statement
Declaration Competing of Interest No competing interests have been declared.
Citations
This article has been cited 1 times.- Wang C, Zeng Y, Wang J, Wang T, Li X, Shen Z, Meng J, Yao X. A genome-wide association study of the racing performance traits in Yili horses based on Blink and FarmCPU models. Sci Rep 2024 Nov 12;14(1):27648.
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