Progesterone determination in equine plasma using different immunoassays.
Abstract: Several assay systems (3H radioimmunoassay (RIA) with and without extraction; microplate enzyme-linked immunoassay (ELISA); qualitative ELISA (tube test)] were used to measure plasma progesterone concentration in mare plasma. The direct RIA showed a close correlation (R = 0.94) with the extraction RIA. The direct RIA and the microplate ELISA were compared in two different studies. In the first study 1155 samples of postpartum mares were used for progesterone determination with both assays. The ELISA resulted in more elevated values both in oestrus and dioestrus (0.19+/-0.3 and 2.44+/-3.62 nmol/l for oestrus, n = 436, and 8.94+/-4.29 and 27.88+/-18.34 nmol/l for dioestrus, N = 719, for the RIA and ELISA, respectively, R = 0.71). The evaluation of individual progesterone profiles has revealed that the microplate ELISA detects the time of ovulation at the same time as it is determined by the RIA and clinical examination. The sensitivity and specificity were calculated for different progesterone threshold values. In the second study including 7 non-pregnant, cycling mares the progesterone concentration of 240 samples was determined by both assays. Basal values (Day 0) obtained with the ELISA were higher (1.57 nmol/l) than those of the RIA (0.2 nmol/l). Both curves reached the same maximum concentration (12.11 and 12.45 nmol/l) 5 days after ovulation. The correlation between the RIA and ELISA values was high (R = 0.90). The tube test was compared to the microplate ELISA as reference using 576 plasma samples of 34 non-pregnant, non-cycling mares included in an ovulation induction study. Of these samples 118 had higher and 458 had lower values than 3.18 nmol/l. In most cases the tube test was in complete agreement with the microplate ELISA. The sensitivity, specificity, + predictive and - predictive values for the tube test were 79.7%, 95.4%, 81.7% and 94.8%, respectively.
Publication Date: 1998-08-26 PubMed ID: 9713151
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- Journal Article
- Research Support
- Non-U.S. Gov't
- Review
Summary
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This research explores the comparison of different assay systems in determining the concentration of progesterone in mare plasma. The results show a close correlation between different methods, shedding light on their relative effectiveness and potential applications in equine reproductive studies.
Research Methods
- Several assay systems were used in this research. These include 3H radioimmunoassay (RIA) with and without extraction; microplate enzyme-linked immunoassay (ELISA); and qualitative ELISA (tube test). These assays were utilized to measure the concentration of progesterone in mare plasma.
- The direct RIA was closely compared with the extraction RIA, and the comparison also included direct RIA and the microplate ELISA.
- The effectiveness of these assays was gauged by using them on a total of 1155 samples of postpartum mare plasma in one study and 240 samples from 7 non-pregnant, cycling mares in another study.
Research Findings
- The results showed a close correlation (R = 0.94) between the direct RIA and the extraction RIA. This suggests that these two methods can be used interchangeably for determining progesterone concentration.
- When the direct RIA and the microplate ELISA were compared, the ELISA resulted in more elevated values in both estrus and diestrus stages of reproductive cycle.
- It was also observed that the microplate ELISA was able to detect the time of ovulation at the same time as the RIA and clinical examination. This suggests that the microplate ELISA can be a reliable method for ovulation detection.
- The sensitivity and specificity of these assays were calculated for different progesterone threshold values, providing further means to compare the different methods.
- Higher basal values were obtained with the ELISA than with the RIA.
Comparison with Tube Test
- The research further included a comparison of the tube test to the microplate ELISA, using 576 plasma samples from 34 non-pregnant, non-cycling mares as part of an ovulation induction study.
- In most cases, the tube test was in complete agreement with the microplate ELISA, suggesting their mutual compatibility for the determination of progesterone concentration in mare plasma.
Cite This Article
APA
Nagy P, Solti L, Kulcsár M, Reiczigel J, Huszenicza G, Abaváry K, Wölfling A.
(1998).
Progesterone determination in equine plasma using different immunoassays.
Acta Vet Hung, 46(4), 501-513.
Publication
Researcher Affiliations
- Department of Obstetrics and Reproduction, University of Veterinary Science, Budapest, Hungary.
MeSH Terms
- Animals
- Enzyme-Linked Immunosorbent Assay
- Estrus
- Female
- Horses / blood
- Immunoassay / classification
- Postpartum Period
- Pregnancy
- Pregnancy, Animal
- Progesterone / blood
- Radioimmunoassay
Citations
This article has been cited 4 times.- Aleman M, McCue PM, Chigerwe M, Madigan JE. Plasma concentrations of steroid precursors, steroids, neuroactive steroids, and neurosteroids in healthy neonatal foals from birth to 7 days of age.. J Vet Intern Med 2019 Sep;33(5):2286-2293.
- Gehlen H, Shety T, El-Zahar H, Hofheinz I. Measurement of plasma endothelin-1 concentration in healthy horses and horses with cardiac disease during rest and after exercise.. J Vet Med Sci 2019 Feb 28;81(2):263-268.
- Colazo MG, Ambrose DJ, Kastelic JP, Small JA. Comparison of 2 enzyme immunoassays and a radioimmunoassay for measurement of progesterone concentrations in bovine plasma, skim milk, and whole milk.. Can J Vet Res 2008 Jan;72(1):32-6.
- Bayemi PH, Nsongka VM, Perera BM, Cavestany D, Webb EC. Validation of a human progesterone enzyme immunoassay (EIA) kit for use on serum of cattle in Cameroon.. Trop Anim Health Prod 2007 Jun;39(5):335-8.
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