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Advanced drug delivery reviews2014; 84; 240-256; doi: 10.1016/j.addr.2014.11.023

Progress in cell-based therapies for tendon repair.

Abstract: The last decade has seen significant developments in cell therapies, based on permanently differentiated, reprogrammed or engineered stem cells, for tendon injuries and degenerative conditions. In vitro studies assess the influence of biophysical, biochemical and biological signals on tenogenic phenotype maintenance and/or differentiation towards tenogenic lineage. However, the ideal culture environment has yet to be identified due to the lack of standardised experimental setup and readout system. Bone marrow mesenchymal stem cells and tenocytes/dermal fibroblasts appear to be the cell populations of choice for clinical translation in equine and human patients respectively based on circumstantial, rather than on hard evidence. Collaborative, inter- and multi-disciplinary efforts are expected to provide clinically relevant and commercially viable cell-based therapies for tendon repair and regeneration in the years to come.
Copyright © 2014 Elsevier B.V. All rights reserved.
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Publication Date: 2014-12-24 PubMed ID: 25543005DOI: 10.1016/j.addr.2014.11.023Google Scholar: Lookup
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  • Research Support
  • Non-U.S. Gov't
  • Review

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research article delves into the progress made over the last decade in the use of cell therapies to treat tendon injuries and conditions. It explains how stem cells have been manipulated and modified for these treatments, and while some results are promising, further work is needed to identify the best approach.

Research Focus

  • The paper focuses on recent advances in the use of cell therapies for tendon injuries and degenerative conditions. Specifically, the use of permanently differentiated, reprogrammed, or engineered stem cells is explored.

Current Findings

  • In lab conditions, researchers have been working to understand how different types of signals – biophysical, biochemical, and biological – can influence a cell’s abilities to either maintain its tenogenic (tendon-forming) properties or differentiate into a tenogenic lineage.
  • Presently, the “ideal culture environment”, that is, the best conditions under which to grow these cells, has not been found since there is no standardization in the experimental setup and readout system across different researches.

Specific Cell Types

  • The paper states that bone marrow mesenchymal stem cells and tenocytes/dermal fibroblasts appear to be the cell populations of choice for clinical trials for equine and human patients. This inference is based on circumstantial evidence rather than hard scientific data.

Future Direction

  • The research acknowledges the need for further study and greater collaboration between multiple scientific disciplines. This cooperation is critical to advance the field and eventually develop clinically relevant and commercially viable cell-based therapies that can effectively repair and regenerate tendons.

Cite This Article

APA
Gaspar D, Spanoudes K, Holladay C, Pandit A, Zeugolis D. (2014). Progress in cell-based therapies for tendon repair. Adv Drug Deliv Rev, 84, 240-256. https://doi.org/10.1016/j.addr.2014.11.023

Publication

Advanced drug delivery reviews
ISSN: 1872-8294
NlmUniqueID: 8710523
Country: Netherlands
Language: English
Volume: 84
Pages: 240-256
PII: S0169-409X(14)00307-X

Researcher Affiliations

Gaspar, Diana
  • Network of Excellence for Functional Biomaterials (NFB), National University of Ireland, Galway (NUI Galway), Galway, Ireland.
Spanoudes, Kyriakos
  • Network of Excellence for Functional Biomaterials (NFB), National University of Ireland, Galway (NUI Galway), Galway, Ireland.
Holladay, Carolyn
  • Network of Excellence for Functional Biomaterials (NFB), National University of Ireland, Galway (NUI Galway), Galway, Ireland.
Pandit, Abhay
  • Network of Excellence for Functional Biomaterials (NFB), National University of Ireland, Galway (NUI Galway), Galway, Ireland.
Zeugolis, Dimitrios
  • Network of Excellence for Functional Biomaterials (NFB), National University of Ireland, Galway (NUI Galway), Galway, Ireland. Electronic address: dimitrios.zeugolis@nuigalway.ie.

MeSH Terms

  • Animals
  • Cell Culture Techniques / methods
  • Cell- and Tissue-Based Therapy / methods
  • Cell- and Tissue-Based Therapy / veterinary
  • Humans
  • Regeneration / physiology
  • Tendons / physiology

Citations

This article has been cited 74 times.
  1. Jeannerat A, Meuli J, Peneveyre C, Jaccoud S, Chemali M, Thomas A, Liao Z, Abdel-Sayed P, Scaletta C, Hirt-Burri N, Applegate LA, Raffoul W, Laurent A. Bio-Enhanced Neoligaments Graft Bearing FE002 Primary Progenitor Tenocytes: Allogeneic Tissue Engineering & Surgical Proofs-of-Concept for Hand Ligament Regenerative Medicine. Pharmaceutics 2023 Jul 3;15(7).
    doi: 10.3390/pharmaceutics15071873pubmed: 37514060google scholar: lookup
  2. Park D, Lee SJ, Choi DK, Park JW. Therapeutic Agent-Loaded Fibrous Scaffolds for Biomedical Applications. Pharmaceutics 2023 May 17;15(5).
    doi: 10.3390/pharmaceutics15051522pubmed: 37242764google scholar: lookup
  3. Kroner-Weigl N, Chu J, Rudert M, Alt V, Shukunami C, Docheva D. Dexamethasone Is Not Sufficient to Facilitate Tenogenic Differentiation of Dermal Fibroblasts in a 3D Organoid Model. Biomedicines 2023 Mar 3;11(3).
    doi: 10.3390/biomedicines11030772pubmed: 36979751google scholar: lookup
  4. Senesi L, De Francesco F, Marchesini A, Pangrazi PP, Bertolini M, Riccio V, Riccio M. Efficacy of Adipose-Derived Mesenchymal Stem Cells and Stromal Vascular Fraction Alone and Combined to Biomaterials in Tendinopathy or Tendon Injury: Systematic Review of Current Concepts. Medicina (Kaunas) 2023 Jan 31;59(2).
    doi: 10.3390/medicina59020273pubmed: 36837474google scholar: lookup
  5. Tsiapalis D, Kearns S, Kelly JL, Zeugolis DI. Growth factor and macromolecular crowding supplementation in human tenocyte culture. Biomater Biosyst 2021 Mar;1:100009.
    doi: 10.1016/j.bbiosy.2021.100009pubmed: 36825160google scholar: lookup
  6. Hu J, Liu S, Fan C. Applications of functionally-adapted hydrogels in tendon repair. Front Bioeng Biotechnol 2023;11:1135090.
    doi: 10.3389/fbioe.2023.1135090pubmed: 36815891google scholar: lookup
  7. Smith EJ, Beaumont RE, McClellan A, Sze C, Palomino Lago E, Hazelgrove L, Dudhia J, Smith RKW, Guest DJ. Tumour necrosis factor alpha, interleukin 1 beta and interferon gamma have detrimental effects on equine tenocytes that cannot be rescued by IL-1RA or mesenchymal stromal cell-derived factors. Cell Tissue Res 2023 Mar;391(3):523-544.
    doi: 10.1007/s00441-022-03726-6pubmed: 36543895google scholar: lookup
  8. Liu X, Li Y, Wang S, Lu M, Zou J, Shi Z, Xu B, Wang W, Hu B, Jin T, Wu F, Liu S, Fan C. PDGF-loaded microneedles promote tendon healing through p38/cyclin D1 pathway mediated angiogenesis. Mater Today Bio 2022 Dec;16:100428.
    doi: 10.1016/j.mtbio.2022.100428pubmed: 36238965google scholar: lookup
  9. Xu L, Chen Z, Geng T, Ru B, Wan Q, Zhang J, Li S, Cai W. Irisin promotes the proliferation and tenogenic differentiation of rat tendon-derived stem/progenitor cells via activating YAP/TAZ. In Vitro Cell Dev Biol Anim 2022 Sep;58(8):658-668.
    doi: 10.1007/s11626-022-00699-2pubmed: 36125694google scholar: lookup
  10. Rampin A, Skoufos I, Raghunath M, Tzora A, Diakakis N, Prassinos N, Zeugolis DI. Allogeneic Serum and Macromolecular Crowding Maintain Native Equine Tenocyte Function in Culture. Cells 2022 May 5;11(9).
    doi: 10.3390/cells11091562pubmed: 35563866google scholar: lookup
  11. Wright AL, Righelli L, Broomhall TJ, Lamont HC, El Haj AJ. Magnetic Nanoparticle-Mediated Orientation of Collagen Hydrogels for Engineering of Tendon-Mimetic Constructs. Front Bioeng Biotechnol 2022;10:797437.
    doi: 10.3389/fbioe.2022.797437pubmed: 35372293google scholar: lookup
  12. Wang K, Cheng L, He B. Therapeutic effects of asperosaponin VI in rabbit tendon disease. Regen Ther 2022 Jun;20:1-8.
    doi: 10.1016/j.reth.2022.02.001pubmed: 35310016google scholar: lookup
  13. Song K, Jiang T, Pan P, Yao Y, Jiang Q. Exosomes from tendon derived stem cells promote tendon repair through miR-144-3p-regulated tenocyte proliferation and migration. Stem Cell Res Ther 2022 Feb 23;13(1):80.
    doi: 10.1186/s13287-022-02723-4pubmed: 35197108google scholar: lookup
  14. Russo V, El Khatib M, Prencipe G, Citeroni MR, Faydaver M, Mauro A, Berardinelli P, Cerveró-Varona A, Haidar-Montes AA, Turriani M, Di Giacinto O, Raspa M, Scavizzi F, Bonaventura F, Stöckl J, Barboni B. Tendon Immune Regeneration: Insights on the Synergetic Role of Stem and Immune Cells during Tendon Regeneration. Cells 2022 Jan 27;11(3).
    doi: 10.3390/cells11030434pubmed: 35159244google scholar: lookup
  15. Han Q, Wang S, Chen D, Gan D, Wang T. Exosomes derived from human umbilical cord mesenchymal stem cells reduce tendon injuries via the miR-27b-3p/ARHGAP5/RhoA signaling pathway. Acta Biochim Biophys Sin (Shanghai) 2022 Jan 25;54(2):232-242.
    doi: 10.3724/abbs.2021026pubmed: 35130628google scholar: lookup
  16. Wellings EP, Huang TC, Li J, Peterson TE, Hooke AW, Rosenbaum A, Zhao CD, Behfar A, Moran SL, Houdek MT. Intrinsic Tendon Regeneration After Application of Purified Exosome Product: An In Vivo Study. Orthop J Sports Med 2021 Dec;9(12):23259671211062929.
    doi: 10.1177/23259671211062929pubmed: 34988236google scholar: lookup
  17. Yang J, Kang Y, Zhao W, Jiang J, Jiang Y, Zhao B, Jiao M, Yuan B, Zhao J, Ma B. Evaluation of patches for rotator cuff repair: A systematic review and meta-analysis based on animal studies. Bioact Mater 2022 Apr;10:474-491.
    doi: 10.1016/j.bioactmat.2021.08.016pubmed: 34901561google scholar: lookup
  18. Ryan CNM, Pugliese E, Shologu N, Gaspar D, Rooney P, Islam MN, O'Riordan A, Biggs MJ, Griffin MD, Zeugolis DI. A combined physicochemical approach towards human tenocyte phenotype maintenance. Mater Today Bio 2021 Sep;12:100130.
    doi: 10.1016/j.mtbio.2021.100130pubmed: 34632361google scholar: lookup
  19. Palumbo Piccionello A, Riccio V, Senesi L, Volta A, Pennasilico L, Botto R, Rossi G, Tambella AM, Galosi L, Marini C, Vullo C, Gigante A, Zavan B, De Francesco F, Riccio M. Adipose micro-grafts enhance tendinopathy healing in ovine model: An in vivo experimental perspective study. Stem Cells Transl Med 2021 Nov;10(11):1544-1560.
    doi: 10.1002/sctm.20-0496pubmed: 34398527google scholar: lookup
  20. Zhang Z, Li Y, Zhang T, Shi M, Song X, Yang S, Liu H, Zhang M, Cui Q, Li Z. Hepatocyte Growth Factor-Induced Tendon Stem Cell Conditioned Medium Promotes Healing of Injured Achilles Tendon. Front Cell Dev Biol 2021;9:654084.
    doi: 10.3389/fcell.2021.654084pubmed: 33898452google scholar: lookup
  21. Yang F, Richardson DW. Comparative Analysis of Tenogenic Gene Expression in Tenocyte-Derived Induced Pluripotent Stem Cells and Bone Marrow-Derived Mesenchymal Stem Cells in Response to Biochemical and Biomechanical Stimuli. Stem Cells Int 2021;2021:8835576.
    doi: 10.1155/2021/8835576pubmed: 33510795google scholar: lookup
  22. Tsai SL, Nödl MT, Galloway JL. Bringing tendon biology to heel: Leveraging mechanisms of tendon development, healing, and regeneration to advance therapeutic strategies. Dev Dyn 2021 Mar;250(3):393-413.
    doi: 10.1002/dvdy.269pubmed: 33169466google scholar: lookup
  23. Chu J, Lu M, Pfeifer CG, Alt V, Docheva D. Rebuilding Tendons: A Concise Review on the Potential of Dermal Fibroblasts. Cells 2020 Sep 8;9(9).
    doi: 10.3390/cells9092047pubmed: 32911760google scholar: lookup
  24. Ciardulli MC, Marino L, Lamparelli EP, Guida M, Forsyth NR, Selleri C, Della Porta G, Maffulli N. Dose-Response Tendon-Specific Markers Induction by Growth Differentiation Factor-5 in Human Bone Marrow and Umbilical Cord Mesenchymal Stem Cells. Int J Mol Sci 2020 Aug 17;21(16).
    doi: 10.3390/ijms21165905pubmed: 32824547google scholar: lookup
  25. Desai S, Jayasuriya CT. Implementation of Endogenous and Exogenous Mesenchymal Progenitor Cells for Skeletal Tissue Regeneration and Repair. Bioengineering (Basel) 2020 Aug 4;7(3).
    doi: 10.3390/bioengineering7030086pubmed: 32759659google scholar: lookup
  26. Ciardulli MC, Marino L, Lovecchio J, Giordano E, Forsyth NR, Selleri C, Maffulli N, Porta GD. Tendon and Cytokine Marker Expression by Human Bone Marrow Mesenchymal Stem Cells in a Hyaluronate/Poly-Lactic-Co-Glycolic Acid (PLGA)/Fibrin Three-Dimensional (3D) Scaffold. Cells 2020 May 20;9(5).
    doi: 10.3390/cells9051268pubmed: 32443833google scholar: lookup
  27. van den Boom NAC, Winters M, Haisma HJ, Moen MH. Efficacy of Stem Cell Therapy for Tendon Disorders: A Systematic Review. Orthop J Sports Med 2020 Apr;8(4):2325967120915857.
    doi: 10.1177/2325967120915857pubmed: 32440519google scholar: lookup
  28. Ghayemi N, Sarrafzadeh-Rezaei F, Malekinejad H, Behfar M, Farshid AA. Effects of rabbit pinna-derived blastema cells on tendon healing. Iran J Basic Med Sci 2020 Jan;23(1):13-19.
    doi: 10.22038/IJBMS.2019.29102.7045pubmed: 32395204google scholar: lookup
  29. Komura S, Satake T, Goto A, Aoki H, Shibata H, Ito K, Hirakawa A, Yamada Y, Akiyama H. Induced pluripotent stem cell-derived tenocyte-like cells promote the regeneration of injured tendons in mice. Sci Rep 2020 Mar 4;10(1):3992.
    doi: 10.1038/s41598-020-61063-6pubmed: 32132649google scholar: lookup
  30. Russo V, El Khatib M, di Marcantonio L, Ancora M, Wyrwa R, Mauro A, Walter T, Weisser J, Citeroni MR, Lazzaro F, Di Federico M, Berardinelli P, Cammà C, Schnabelrauch M, Barboni B. Tendon Biomimetic Electrospun PLGA Fleeces Induce an Early Epithelial-Mesenchymal Transition and Tenogenic Differentiation on Amniotic Epithelial Stem Cells. Cells 2020 Jan 27;9(2).
    doi: 10.3390/cells9020303pubmed: 32012741google scholar: lookup
  31. Evrova O, Kellenberger D, Calcagni M, Vogel V, Buschmann J. Supporting Cell-Based Tendon Therapy: Effect of PDGF-BB and Ascorbic Acid on Rabbit Achilles Tenocytes in Vitro. Int J Mol Sci 2020 Jan 10;21(2).
    doi: 10.3390/ijms21020458pubmed: 31936891google scholar: lookup
  32. Kim SE, Kim JG, Park K. Biomaterials for the Treatment of Tendon Injury. Tissue Eng Regen Med 2019 Oct;16(5):467-477.
    doi: 10.1007/s13770-019-00217-8pubmed: 31624702google scholar: lookup
  33. Li W, Midgley AC, Bai Y, Zhu M, Chang H, Zhu W, Wang L, Wang Y, Wang H, Kong D. Subcutaneously engineered autologous extracellular matrix scaffolds with aligned microchannels for enhanced tendon regeneration: Aligned microchannel scaffolds for tendon repair. Biomaterials 2019 Dec;224:119488.
    doi: 10.1016/j.biomaterials.2019.119488pubmed: 31562997google scholar: lookup
  34. Yang F, Zhang A, Richardson DW. Regulation of the tenogenic gene expression in equine tenocyte-derived induced pluripotent stem cells by mechanical loading and Mohawk. Stem Cell Res 2019 Aug;39:101489.
    doi: 10.1016/j.scr.2019.101489pubmed: 31277043google scholar: lookup
  35. Costa-Almeida R, Calejo I, Gomes ME. Mesenchymal Stem Cells Empowering Tendon Regenerative Therapies. Int J Mol Sci 2019 Jun 19;20(12).
    doi: 10.3390/ijms20123002pubmed: 31248196google scholar: lookup
  36. Oliva F, Maffulli N, Gissi C, Veronesi F, Calciano L, Fini M, Brogini S, Gallorini M, Antonetti Lamorgese Passeri C, Bernardini R, Cicconi R, Mattei M, Berardi AC. Combined ascorbic acid and T(3) produce better healing compared to bone marrow mesenchymal stem cells in an Achilles tendon injury rat model: a proof of concept study. J Orthop Surg Res 2019 Feb 18;14(1):54.
    doi: 10.1186/s13018-019-1098-9pubmed: 30777116google scholar: lookup
  37. Perucca Orfei C, Viganò M, Pearson JR, Colombini A, De Luca P, Ragni E, Santos-Ruiz L, de Girolamo L. In Vitro Induction of Tendon-Specific Markers in Tendon Cells, Adipose- and Bone Marrow-Derived Stem Cells is Dependent on TGFβ3, BMP-12 and Ascorbic Acid Stimulation. Int J Mol Sci 2019 Jan 3;20(1).
    doi: 10.3390/ijms20010149pubmed: 30609804google scholar: lookup
  38. Efird WM, Fletcher AG, Draeger RW, Spang JT, Dahners LE, Weinhold PS. Deferoxamine-Soaked Suture Improves Angiogenesis and Repair Potential After Acute Injury of the Chicken Achilles Tendon. Orthop J Sports Med 2018 Oct;6(10):2325967118802792.
    doi: 10.1177/2325967118802792pubmed: 30370309google scholar: lookup
  39. Wang D, Jiang X, Lu A, Tu M, Huang W, Huang P. BMP14 induces tenogenic differentiation of bone marrow mesenchymal stem cells in vitro. Exp Ther Med 2018 Aug;16(2):1165-1174.
    doi: 10.3892/etm.2018.6293pubmed: 30116367google scholar: lookup
  40. Kim JH, Jo CH, Kim HR, Hwang YI. Comparison of Immunological Characteristics of Mesenchymal Stem Cells from the Periodontal Ligament, Umbilical Cord, and Adipose Tissue. Stem Cells Int 2018;2018:8429042.
    doi: 10.1155/2018/8429042pubmed: 29760736google scholar: lookup
  41. Lin D, Alberton P, Caceres MD, Volkmer E, Schieker M, Docheva D. Tenomodulin is essential for prevention of adipocyte accumulation and fibrovascular scar formation during early tendon healing. Cell Death Dis 2017 Oct 12;8(10):e3116.
    doi: 10.1038/cddis.2017.510pubmed: 29022912google scholar: lookup
  42. Wu S, Wang Y, Streubel PN, Duan B. Living nanofiber yarn-based woven biotextiles for tendon tissue engineering using cell tri-culture and mechanical stimulation. Acta Biomater 2017 Oct 15;62:102-115.
    doi: 10.1016/j.actbio.2017.08.043pubmed: 28864251google scholar: lookup
  43. di Giacomo V, Berardocco M, Gallorini M, Oliva F, Colosimo A, Cataldi A, Maffulli N, Berardi AC. Combined supplementation of ascorbic acid and thyroid hormone T(3) affects tenocyte proliferation. The effect of ascorbic acid in the production of nitric oxide. Muscles Ligaments Tendons J 2017 Jan-Mar;7(1):11-18.
    doi: 10.11138/mltj/2017.7.1.011pubmed: 28717606google scholar: lookup
  44. Testa S, Costantini M, Fornetti E, Bernardini S, Trombetta M, Seliktar D, Cannata S, Rainer A, Gargioli C. Combination of biochemical and mechanical cues for tendon tissue engineering. J Cell Mol Med 2017 Nov;21(11):2711-2719.
    doi: 10.1111/jcmm.13186pubmed: 28470843google scholar: lookup
  45. Viganò M, Perucca Orfei C, Colombini A, Stanco D, Randelli P, Sansone V, de Girolamo L. Different culture conditions affect the growth of human tendon stem/progenitor cells (TSPCs) within a mixed tendon cells (TCs) population. J Exp Orthop 2017 Dec;4(1):8.
    doi: 10.1186/s40634-017-0082-8pubmed: 28244027google scholar: lookup
  46. Zarychta-Wiśniewska W, Burdzinska A, Kulesza A, Gala K, Kaleta B, Zielniok K, Siennicka K, Sabat M, Paczek L. Bmp-12 activates tenogenic pathway in human adipose stem cells and affects their immunomodulatory and secretory properties. BMC Cell Biol 2017 Feb 18;18(1):13.
    doi: 10.1186/s12860-017-0129-9pubmed: 28214472google scholar: lookup
  47. Yin Z, Hu JJ, Yang L, Zheng ZF, An CR, Wu BB, Zhang C, Shen WL, Liu HH, Chen JL, Heng BC, Guo GJ, Chen X, Ouyang HW. Single-cell analysis reveals a nestin(+) tendon stem/progenitor cell population with strong tenogenic potentiality. Sci Adv 2016 Nov;2(11):e1600874.
    doi: 10.1126/sciadv.1600874pubmed: 28138519google scholar: lookup
  48. Viganò M, Sansone V, d'Agostino MC, Romeo P, Perucca Orfei C, de Girolamo L. Mesenchymal stem cells as therapeutic target of biophysical stimulation for the treatment of musculoskeletal disorders. J Orthop Surg Res 2016 Dec 16;11(1):163.
    doi: 10.1186/s13018-016-0496-5pubmed: 27986082google scholar: lookup
  49. Hofer HR, Tuan RS. Secreted trophic factors of mesenchymal stem cells support neurovascular and musculoskeletal therapies. Stem Cell Res Ther 2016 Sep 9;7(1):131.
    doi: 10.1186/s13287-016-0394-0pubmed: 27612948google scholar: lookup
  50. Walden G, Liao X, Donell S, Raxworthy MJ, Riley GP, Saeed A. A Clinical, Biological, and Biomaterials Perspective into Tendon Injuries and Regeneration. Tissue Eng Part B Rev 2017 Feb;23(1):44-58.
    doi: 10.1089/ten.TEB.2016.0181pubmed: 27596929google scholar: lookup
  51. Chen X, Zou X, Yu G, Fu X, Cao T, Xiao Y, Ouyang H. [Expert consensus on induction of human embryonic stem cells into tenocytes]. Zhejiang Da Xue Xue Bao Yi Xue Ban 2016 Mar;45(2):105-11.
    doi: 10.3785/j.issn.1008-9292.2016.03.01pubmed: 27273982google scholar: lookup
  52. Abbah SA, Thomas D, Browne S, O'Brien T, Pandit A, Zeugolis DI. Co-transfection of decorin and interleukin-10 modulates pro-fibrotic extracellular matrix gene expression in human tenocyte culture. Sci Rep 2016 Feb 10;6:20922.
    doi: 10.1038/srep20922pubmed: 26860065google scholar: lookup
  53. Youngstrom DW, Barrett JG. Engineering Tendon: Scaffolds, Bioreactors, and Models of Regeneration. Stem Cells Int 2016;2016:3919030.
    doi: 10.1155/2016/3919030pubmed: 26839559google scholar: lookup
  54. Govoni M, Muscari C, Lovecchio J, Guarnieri C, Giordano E. Mechanical Actuation Systems for the Phenotype Commitment of Stem Cell-Based Tendon and Ligament Tissue Substitutes. Stem Cell Rev Rep 2016 Apr;12(2):189-201.
    doi: 10.1007/s12015-015-9640-6pubmed: 26661573google scholar: lookup
  55. Dai L, Hu X, Zhang X, Zhu J, Zhang J, Fu X, Duan X, Ao Y, Zhou C. Different tenogenic differentiation capacities of different mesenchymal stem cells in the presence of BMP-12. J Transl Med 2015 Jun 24;13:200.
    doi: 10.1186/s12967-015-0560-7pubmed: 26104414google scholar: lookup
  56. Rossoni A, Lauretta G, Kearns S, Zeugolis DI. Comparative analysis of different macromolecular crowding agents in human tenocyte cultures. Biomater Biosyst 2026 Mar;21:100127.
    doi: 10.1016/j.bbiosy.2026.100127pubmed: 41586349google scholar: lookup
  57. Djalali-Cuevas A, Rettel M, Stein F, Savitski M, Gkiatas I, Korompilias A, Skoufos I, Tzora A, Prassinos N, Diakakis N, Zeugolis DI. Multifactorial bioengineering in the tendon context to maintain tenocyte phenotype and to direct dermal fibroblasts towards tenogenic lineage. Bioact Mater 2026 Feb;56:743-755.
    doi: 10.1016/j.bioactmat.2025.11.008pubmed: 41473828google scholar: lookup
  58. Liu H, Zhang A, Shi M, Zhang J, Zhang T, Lu W, Zhang M, Zhang Z, Wu Y, Miao Y, Wang S, Hou L, Cui Q, Li Z. The treatment of acute tendon injury with small extracellular vesicles originating from TNFAIP6(-) ADSCs subpopoulation both in vitro and in vivo. Stem Cell Res Ther 2025 Dec 30;17(1):61.
    doi: 10.1186/s13287-025-04789-2pubmed: 41469712google scholar: lookup
  59. Chen B, Zhao Y, Guo S, Tang C, Xu B, Zhou M, Chen Q, Ma L, Lyu J, Guo L, Wang Y. Tendon Tissue Engineering: Pathophysiological Mechanism and Bioengineering Therapy of Tendinopathy. Int J Nanomedicine 2025;20:12529-12571.
    doi: 10.2147/IJN.S550439pubmed: 41127789google scholar: lookup
  60. De Luca P, Grieco G, Bargeri S, Colombo C, Guida S, Taiana MM, de Girolamo L. The interplay between metabolic disorders and tendinopathies: Systematic review and meta-analysis. J Exp Orthop 2025 Jul;12(3):e70429.
    doi: 10.1002/jeo2.70429pubmed: 40937086google scholar: lookup
  61. Wolint P, Miescher I, Mechakra A, Jäger P, Rieber J, Calcagni M, Giovanoli P, Vogel V, Snedeker JG, Buschmann J. Therapeutic Potential of Mesenchymal Stem Cell and Tenocyte Secretomes for Tendon Repair: Proteomic Profiling and Functional Characterization In Vitro and In Ovo. Int J Mol Sci 2025 Apr 11;26(8).
    doi: 10.3390/ijms26083622pubmed: 40332130google scholar: lookup
  62. Guillaumin S, Rossoni A, Zeugolis D. State-of the-art and future perspective in co-culture systems for tendon engineering. Biomater Biosyst 2025 Mar;17:100110.
    doi: 10.1016/j.bbiosy.2025.100110pubmed: 40130022google scholar: lookup
  63. Zhou H, Chen Y, Yan W, Chen X, Zi Y. Advances and challenges in biomaterials for tendon and enthesis repair. Bioact Mater 2025 May;47:531-545.
    doi: 10.1016/j.bioactmat.2025.01.001pubmed: 40062342google scholar: lookup
  64. Später T, Del Rio P, Shelest O, Wechsler JT, Kaneda G, Chavez M, Sheyn J, Yu V, Metzger W, Huang D, Metzger M, Tawackoli W, Sheyn D. Collagen scaffold-seeded iTenocytes accelerate the healing and functional recovery of Achilles tendon defects in a rat model. Front Bioeng Biotechnol 2024;12:1407729.
    doi: 10.3389/fbioe.2024.1407729pubmed: 39713100google scholar: lookup
  65. Zhang W, Rao Y, Wong SH, Wu Y, Zhang Y, Yang R, Tsui SK, Ker DFE, Mao C, Frith JE, Cao Q, Tuan RS, Wang DM. Transcriptome-Optimized Hydrogel Design of a Stem Cell Niche for Enhanced Tendon Regeneration. Adv Mater 2025 Jan;37(2):e2313722.
    doi: 10.1002/adma.202313722pubmed: 39417770google scholar: lookup
  66. Nosrati H, Fallah Tafti M, Aghamollaei H, Bonakdar S, Moosazadeh Moghaddam M. Directed Differentiation of Adipose-Derived Stem Cells Using Imprinted Cell-Like Topographies as a Growth Factor-Free Approach. Stem Cell Rev Rep 2024 Oct;20(7):1752-1781.
    doi: 10.1007/s12015-024-10767-7pubmed: 39066936google scholar: lookup
  67. Roets B, Abrahamse H, Crous A. The Application of Photobiomodulation on Mesenchymal Stem Cells and its Potential Use for Tenocyte Differentiation. Curr Stem Cell Res Ther 2025;20(3):232-245.
    doi: 10.2174/011574888X295488240319111911pubmed: 38847377google scholar: lookup
  68. Dec P, Żyłka M, Burszewski P, Modrzejewski A, Pawlik A. Recent Advances in the Use of Stem Cells in Tissue Engineering and Adjunct Therapies for Tendon Reconstruction and Future Perspectives. Int J Mol Sci 2024 Apr 19;25(8).
    doi: 10.3390/ijms25084498pubmed: 38674084google scholar: lookup
  69. DeFoor MT, Cognetti DJ, Yuan TT, Sheean AJ. Treatment of Tendon Injuries in the Servicemember Population across the Spectrum of Pathology: From Exosomes to Bioinductive Scaffolds. Bioengineering (Basel) 2024 Feb 5;11(2).
    doi: 10.3390/bioengineering11020158pubmed: 38391644google scholar: lookup
  70. Djalali-Cuevas A, Rettel M, Stein F, Savitski M, Kearns S, Kelly J, Biggs M, Skoufos I, Tzora A, Prassinos N, Diakakis N, Zeugolis DI. Macromolecular crowding in human tenocyte and skin fibroblast cultures: A comparative analysis. Mater Today Bio 2024 Apr;25:100977.
    doi: 10.1016/j.mtbio.2024.100977pubmed: 38322661google scholar: lookup
  71. De Castilho T, Rosa GDS, Stievani FC, Apolônio EVP, Pfeifer JPH, Altheman VG, Palialogo V, Santos NJD, Fonseca-Alves CE, Alves ALG. Biocompatibility of hydrogel derived from equine tendon extracellular matrix in horses subcutaneous tissue. Front Bioeng Biotechnol 2023;11:1296743.
    doi: 10.3389/fbioe.2023.1296743pubmed: 38260745google scholar: lookup
  72. Zhu Y, Yan J, Zhang H, Cui G. Bone marrow mesenchymal stem cell‑derived exosomes: A novel therapeutic agent for tendon‑bone healing (Review). Int J Mol Med 2023 Dec;52(6).
    doi: 10.3892/ijmm.2023.5324pubmed: 37937691google scholar: lookup
  73. Huang L, Chen L, Chen H, Wang M, Jin L, Zhou S, Gao L, Li R, Li Q, Wang H, Zhang C, Wang J. Biomimetic Scaffolds for Tendon Tissue Regeneration. Biomimetics (Basel) 2023 Jun 9;8(2).
    doi: 10.3390/biomimetics8020246pubmed: 37366841google scholar: lookup
  74. Kaneda G, Chan JL, Castaneda CM, Papalamprou A, Sheyn J, Shelest O, Huang D, Kluser N, Yu V, Ignacio GC, Gertych A, Yoshida R, Metzger MF, Tawackoli W, Vernengo A, Sheyn D. iPSC-derived tenocytes seeded on microgrooved 3D printed scaffolds for Achilles tendon regeneration. J Orthop Res 2023 Oct;41(10):2205-2220.
    doi: 10.1002/jor.25554pubmed: 36961351google scholar: lookup
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