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Veterinary medicine international2017; 2017; 6059485; doi: 10.1155/2017/6059485

Proinflammatory and Anabolic Gene Expression Effects of Platelet-Rich Gel Supernatants on Equine Synovial Membrane Explants Challenged with Lipopolysaccharide.

Abstract: Platelet-rich plasma (PRP) preparations are used in horses with osteoarthritis (OA). However, some controversies remain regarding the ideal concentration of platelets and leukocytes to produce an adequate anti-inflammatory and anabolic response in the synovial membrane. The aims of this study were to study the influence of leukoconcentrated platelet-rich gel (Lc-PRG) and leukoreduced platelet-rich gel (Lr-PRG) supernatants on the quantitative expression of some proinflammatory and anabolic genes in equine synovial membrane explants (SMEs) challenged with lipopolysaccharide (LPS). SMEs from six horses were cultured over 96 h. Then, SMEs were harvested for RNA extraction and quantitative gene expression analysis by RT-qPCR for nuclear factor kappa B (NFκB), matrix metalloproteinase 13 (MMP-13), a disintegrin and metalloproteinase with thrombospondin motifs 4 (ADAMTS-4), collagen type I alpha 1 (COL1A1), collagen type II alpha 1 (COL2A1), and cartilage oligomeric matrix protein (COMP). The 25% and 50% Lc-PRG supernatants led to downregulation of NFκB, MMP-13, ADAMTS-4, COL1A1, COL2A1, and COMP in SMEs. Lr-PRG supernatants (particularly at the 50% concentration) induced downregulation of NFκB, MMP-13, ADAMTS-4, and COL1A1 and upregulation of COL2A1 and COMP. Lr-PRG supernatants should be used for the treatment of inflammatory arthropathies in horses because they have anti-inflammatory and anabolic effects in the synovial membrane.
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Publication Date: 2017-07-06 PubMed ID: 28761774PubMed Central: PMC5518502DOI: 10.1155/2017/6059485Google Scholar: Lookup
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  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research investigates how platelet-rich plasma treatments, with varying concentrations of platelets and leukocytes, can impact anti-inflammatory and anabolic responses in horses suffering from osteoarthritis. It concludes that leukoreduced platelet-rich gel supernatants have beneficial effects on the synovial membrane, making them a suitable treatment for inflammatory arthropathies in horses.

Study Objective

  • The purpose of this study was to examine how leukoconcentrated platelet-rich gel (Lc-PRG) and leukoreduced platelet-rich gel (Lr-PRG) supernatants affect the expression of proinflammatory and anabolic genes in equine synovial membrane explants (SMEs), which had been challenged using lipopolysaccharide (LPS).

Methodology

  • Researchers used SMEs from six horses and cultured them over a period of 96 hours. These SMEs were subsequently harvested for RNA extraction.
  • RT-qPCR was employed for the analysis of quantitative gene expression. The genes investigated were nuclear factor kappa B (NFB), matrix metalloproteinase 13 (MMP-13), a disintegrin and metalloproteinase with thrombospondin motifs 4 (ADAMTS-4), collagen type I alpha 1 (COL1A1), collagen type II alpha 1 (COL2A1), and cartilage oligomeric matrix protein (COMP).

Findings

  • The study found that 25% and 50% Lc-PRG supernatants led to a reduction in the expression of NFB, MMP-13, ADAMTS-4, COL1A1, COL2A1, and COMP genes in SMEs.
  • Interestingly, Lr-PRG supernatants, particularly at concentrations of 50%, induced a decrease in NFB, MMP-13, ADAMTS-4, and COL1A1 gene expression while at the same time increasing the expression of COL2A1 and COMP.

Conclusions

  • The research concluded that Lr-PRG supernatants, owing to their ability to suppress proinflammatory gene expression and stimulate anabolic gene expression, should be used in the treatment of inflammatory arthropathies in horses. This is due to their demonstrated anti-inflammatory and anabolic effects on the synovial membrane.

Cite This Article

APA
Carmona JU, Ríos DL, López C, Álvarez ME, Pérez JE. (2017). Proinflammatory and Anabolic Gene Expression Effects of Platelet-Rich Gel Supernatants on Equine Synovial Membrane Explants Challenged with Lipopolysaccharide. Vet Med Int, 2017, 6059485. https://doi.org/10.1155/2017/6059485

Publication

Veterinary medicine international
ISSN: 2090-8113
NlmUniqueID: 101524203
Country: United States
Language: English
Volume: 2017
Pages: 6059485

Researcher Affiliations

Carmona, Jorge U
  • Grupo de Investigación Terapia Regenerativa, Departamento de Salud Animal, Universidad de Caldas, Manizales, Colombia.
Ríos, Diana L
  • Grupo de Investigación Terapia Regenerativa, Departamento de Salud Animal, Universidad de Caldas, Manizales, Colombia.
López, Catalina
  • Grupo de Investigación Terapia Regenerativa, Departamento de Salud Animal, Universidad de Caldas, Manizales, Colombia.
Álvarez, María E
  • Grupo de Investigación Terapia Regenerativa, Departamento de Salud Animal, Universidad de Caldas, Manizales, Colombia.
  • Grupo de Investigación Biosalud, Departamento de Ciencias Básicas para la Salud, Universidad de Caldas, Manizales, Colombia.
Pérez, Jorge E
  • Grupo de Investigación Biosalud, Departamento de Ciencias Básicas para la Salud, Universidad de Caldas, Manizales, Colombia.

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Citations

This article has been cited 5 times.
  1. Carmona JU, López C, Argüelles D. Addressing Heterogeneity in Equine PRP Therapies: A Scoping Review of Methods, Evidence, and Commercial Validation. Animals (Basel) 2025 Dec 13;15(24).
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  2. Gallego M, López C, Carmona JU. Evaluation of the Pro-, Anti-Inflammatory, and Anabolic Effects of Autologous Platelet-Rich Gel Supernatants in an in vitro Coculture System of Canine Osteoarthritis. Vet Med Int 2022;2022:3377680.
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  3. Camargo Garbin L, Lopez C, Carmona JU. A Critical Overview of the Use of Platelet-Rich Plasma in Equine Medicine Over the Last Decade. Front Vet Sci 2021;8:641818.
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  4. Boffa A, Previtali D, Di Laura Frattura G, Vannini F, Candrian C, Filardo G. Evidence on ankle injections for osteochondral lesions and osteoarthritis: a systematic review and meta-analysis. Int Orthop 2021 Feb;45(2):509-523.
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