Propagation and quantitation of animal herpesviruses in eight cell culture systems.
Abstract: A comparative study was carried out to determine the relative sensitivities of eight different cell culture systems to six different herpesviruses of animals. The cells used were: OFL (ovine fetal lung), ML (mink lung), FK (ferret kidney), PTK-2 (potoroo kidney), TEK (turkey embryo kidney), ED (equine dermal), BT (bovine turbinate), and PK15 (porcine kidney). The viruses tested were: PRV (pseudorabies) of swine, CPHV (caprine herpesvirus), IBRV (infectious bovine rhinotracheitis virus), DN-599 strain of bovine herpesvirus type 4, EHV-1 (equine herpesvirus), and CHV (canine herpesvirus). On the basis of virus titers obtained and the time of appearance of CPE (cytopathic effects), ML cells were found to be the most useful because of their sensitivity to all six viruses tested. BT and OFL cells were also found to be highly sensitive to all viruses with the exception of CHV.
Publication Date: 1988-01-01 PubMed ID: 2846231DOI: 10.1016/0147-9571(88)90023-9Google Scholar: Lookup
The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
- Journal Article
Summary
This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.
This research article compares how effectively eight different cell culture systems can grow and measure six different types of animal herpesviruses. The study found that mink lung (ML) cells were the most sensitive and widely applicable for all the viruses tested.
Introduction
- The study was designed to analyze the relative sensitivities of eight different cell culture systems towards six unique animal herpesviruses.
- This research is crucial as it helps in comprehending which cell cultures are more effective in assisting the growth and quantitation of these viruses, thereby aiding in the diagnosis and research of these diseases.
Cell Culture Systems and Viruses Tested
- The cell culture systems used in the study were OFL (ovine fetal lung), ML (mink lung), FK (ferret kidney), PTK-2 (potoroo kidney), TEK (turkey embryo kidney), ED (equine dermal), BT (bovine turbinate), and PK15 (porcine kidney).
- The viruses that were tested included PRV (pseudorabies) of swine, CPHV (caprine herpesvirus), IBRV (infectious bovine rhinotracheitis virus), DN-599 strain of bovine herpesvirus type 4, EHV-1 (equine herpesvirus), and CHV (canine herpesvirus).
Results
- The results were determined based on the virus titers obtained and the time of appearance of CPE (cytopathic effects), which are visible signs of viral infections in a cell.
- The ML (mink lung) cells were found to be most effective as they were sensitive to all six viruses assessed.
- OFL (ovine fetal lung) and BT (bovine turbinate) cells also displayed high sensitivity towards all the test viruses except for the canine herpesvirus (CHV).
Conclusion
- The outcome of this research assists in creating more effective methods for studying various animal herpesviruses by identifying the most sensitive cell culture systems for propagation and quantitation of these viruses.
- The findings can be utilized to improve diagnostic techniques for these viruses and can further guide research into antiviral drugs and vaccines.
Cite This Article
APA
Peterson RB, Goyal SM.
(1988).
Propagation and quantitation of animal herpesviruses in eight cell culture systems.
Comp Immunol Microbiol Infect Dis, 11(2), 93-98.
https://doi.org/10.1016/0147-9571(88)90023-9 Publication
Researcher Affiliations
- Department of Veterinary Diagnostic Investigation, College of Veterinary Medicine, University of Minnesota, St Paul 55108.
MeSH Terms
- Animals
- Cell Line
- Cytopathogenic Effect, Viral
- Herpesviridae / growth & development
- Herpesviridae Infections / diagnosis
- Herpesviridae Infections / veterinary
- Herpesvirus 1, Bovine / growth & development
- Herpesvirus 1, Canid / growth & development
- Herpesvirus 1, Equid / growth & development
- Herpesvirus 1, Suid / growth & development
- Virus Cultivation
Citations
This article has been cited 12 times.- Franceschi V, Stellari FF, Mangia C, Jacca S, Lavrentiadou S, Cavirani S, Heikenwalder M, Donofrio G. In vivo image analysis of BoHV-4-based vector in mice. PLoS One 2014;9(4):e95779.
- Ruiz-Saenz J, Jaime J, Ramirez G, Vera V. Molecular and in vitro characterization of field isolates of bovine herpesvirus-1. Virol Sin 2012 Feb;27(1):26-37.
- Redaelli M, Franceschi V, Capocefalo A, D'Avella D, Denaro L, Cavirani S, Mucignat-Caretta C, Donofrio G. Herpes simplex virus type 1 thymidine kinase-armed bovine herpesvirus type 4-based vector displays enhanced oncolytic properties in immunocompetent orthotopic syngenic mouse and rat glioma models. Neuro Oncol 2012 Mar;14(3):288-301.
- Redaelli M, Cavaggioni A, Mucignat-Caretta C, Cavirani S, Caretta A, Donofrio G. Transduction of the rat brain by Bovine Herpesvirus 4. Genet Vaccines Ther 2008 Feb 12;6:6.
- Donofrio G, Sartori C, Ravanetti L, Cavirani S, Gillet L, Vanderplasschen A, Taddei S, Flammini CF. Establishment of a bovine herpesvirus 4 based vector expressing a secreted form of the bovine viral diarrhoea virus structural glycoprotein E2 for immunization purposes. BMC Biotechnol 2007 Oct 18;7:68.
- Frampton AR Jr, Stolz DB, Uchida H, Goins WF, Cohen JB, Glorioso JC. Equine herpesvirus 1 enters cells by two different pathways, and infection requires the activation of the cellular kinase ROCK1. J Virol 2007 Oct;81(20):10879-89.
- Donofrio G, Herath S, Sartori C, Cavirani S, Flammini CF, Sheldon IM. Bovine herpesvirus 4 is tropic for bovine endometrial cells and modulates endocrine function. Reproduction 2007 Jul;134(1):183-97.
- Donofrio G, Cavirani S, Vanderplasschen A, Gillet L, Flammini CF. Recombinant bovine herpesvirus 4 (BoHV-4) expressing glycoprotein D of BoHV-1 is immunogenic and elicits serum-neutralizing antibodies against BoHV-1 in a rabbit model. Clin Vaccine Immunol 2006 Nov;13(11):1246-54.
- Donofrio G, Grandi G, Cavirani S, Taddei S, Flammini C. Bovine herpesvirus 4 infects differentiated neuronal cells in culture and establish persistent infection upon selection. J Neurovirol 2004 Apr;10(2):123-30.
- Gillet L, Minner F, Detry B, Farnir F, Willems L, Lambot M, Thiry E, Pastoret PP, Schynts F, Vanderplasschen A. Investigation of the susceptibility of human cell lines to bovine herpesvirus 4 infection: demonstration that human cells can support a nonpermissive persistent infection which protects them against tumor necrosis factor alpha-induced apoptosis. J Virol 2004 Mar;78(5):2336-47.
- Egyed L. Replication of bovine herpesvirus type 4 in human cells in vitro. J Clin Microbiol 1998 Jul;36(7):2109-11.
- Werner U, Kissel T. In-vitro cell culture models of the nasal epithelium: a comparative histochemical investigation of their suitability for drug transport studies. Pharm Res 1996 Jul;13(7):978-88.
Use Nutrition Calculator
Check if your horse's diet meets their nutrition requirements with our easy-to-use tool Check your horse's diet with our easy-to-use tool
Talk to a Nutritionist
Discuss your horse's feeding plan with our experts over a free phone consultation Discuss your horse's diet over a phone consultation
Submit Diet Evaluation
Get a customized feeding plan for your horse formulated by our equine nutritionists Get a custom feeding plan formulated by our nutritionists
