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Proteomics2024; e2300522; doi: 10.1002/pmic.202300522

Proteomics is advancing the understanding of stallion sperm biology.

Abstract: The mammalian ejaculate is very well suited to proteomics studies. As such, research concerning sperm proteomics is offering a huge amount of new information on the biology of spermatozoa. Among domestic animals, horses represent a species of special interest, in which reproductive technologies and a sizeable market of genetic material have grown exponentially in the last decade. Studies using proteomic approaches have been conducted in recent years, showing that proteomics is a potent tool to dig into the biology of the stallion spermatozoa. The aim of this review is to present an overview of the research conducted, and how these studies have improved our knowledge of stallion sperm biology. The main outcomes of the research conducted so far have been an improved knowledge of metabolism, and its importance in sperm functions, the impact of different technologies on the sperm proteome, and the identification of potential biomarkers. Moreover, proteomics of seminal plasma and phosphoproteomics are identified as areas of major interest.
© 2024 The Author(s). Proteomics published by Wiley‐VCH GmbH.
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Publication Date: 2024-05-29 PubMed ID: 38807556DOI: 10.1002/pmic.202300522Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research article reviews studies on stallion sperm proteomics, highlighting its role in enhancing knowledge on their biology, metabolism, and potential use in reproduction technologies.

Overview of the Research

  • The research article is essentially a comprehensive review of studies focused on sperm proteomics, particularly in stallions. Proteomics is the large-scale study of proteins, crucial to understanding the functions of an organism. In this context, it provides significant insights into the biology of spermatozoa, ranging from its metabolism to when it interacts with reproduction enhancing technologies.
  • The interest in stallion sperm comes from a rapidly expanding market and advancements in reproduction technologies in the horse industry. Advancements in these technologies have allowed reproduction even without direct mating, thereby enhancing the value of genetic material from stallions with desirable traits.

Impact of Proteomics on Sperm Biology

  • The use of proteomic approaches has generated interesting and critical information on sperm biology. The information is particularly important in understanding their metabolism, which is critical in sperm function. As a result, researchers have gained a detailed understanding of the energy constructs of the sperm, how they function, and potentially enhance their functionalities.
  • Besides, proteomics has played a crucial role in understanding the impact of various reproductive technologies on the sperm, particularly their protein structure. This knowledge is essential in predicting any potential characteristics that the offsprings might inherit while avoiding any deleterious traits that might arise from the applied technology.

Identification of Potential Biomarkers

  • Proteomics has also been beneficial in identifying potential biomarkers in the sperm. These biomarkers offer unique fingerprints concerning specific characteristics of the sperm, and they can predict given outcomes such as fertility. Biomarkers can be crucial in individual stallion or sperm evaluations, leading to optimal selection during pairing for reproduction.

Areas of Major Interest

  • Finally, the review identifies seminal plasma proteomics and phosphoproteomics as areas of considerable interest in future studies. These two areas have significant potential in deepening understanding of the sperm, given that seminal plasma proteins are involved in sperm capacitation while phosphoproteins play roles in sperm motility.

Cite This Article

APA
Peña FJ, Martín-Cano FE, Becerro-Rey L, Ortega-Ferrusola C, Gaitskell-Phillips G, da Silva-Álvarez E, Gil MC. (2024). Proteomics is advancing the understanding of stallion sperm biology. Proteomics, e2300522. https://doi.org/10.1002/pmic.202300522

Publication

Proteomics
ISSN: 1615-9861
NlmUniqueID: 101092707
Country: Germany
Language: English
Pages: e2300522

Researcher Affiliations

Peña, Fernando J
  • Laboratory of Equine Reproduction and Equine Spermatology, Veterinary Teaching Hospital, Universidad de Extremadura, Cáceres, Spain.
Martín-Cano, Francisco Eduardo
  • Laboratory of Equine Reproduction and Equine Spermatology, Veterinary Teaching Hospital, Universidad de Extremadura, Cáceres, Spain.
Becerro-Rey, Laura
  • Laboratory of Equine Reproduction and Equine Spermatology, Veterinary Teaching Hospital, Universidad de Extremadura, Cáceres, Spain.
Ortega-Ferrusola, Cristina
  • Laboratory of Equine Reproduction and Equine Spermatology, Veterinary Teaching Hospital, Universidad de Extremadura, Cáceres, Spain.
Gaitskell-Phillips, Gemma
  • Laboratory of Equine Reproduction and Equine Spermatology, Veterinary Teaching Hospital, Universidad de Extremadura, Cáceres, Spain.
da Silva-Álvarez, Eva
  • Laboratory of Equine Reproduction and Equine Spermatology, Veterinary Teaching Hospital, Universidad de Extremadura, Cáceres, Spain.
Gil, María Cruz
  • Laboratory of Equine Reproduction and Equine Spermatology, Veterinary Teaching Hospital, Universidad de Extremadura, Cáceres, Spain.

Grant Funding

  • PID2021-122351OB-I00AEI/10.13039/501100011033 / Ministerio de Ciencia e Innovaciu00f3n, Reino de Espau00f1a
  • PRE2022-103090 / Ministerio de Ciencia e Innovaciu00f3n, Reino de Espau00f1a
  • IB 20008 / Consejeru00eda de Empleo Empresa e Innovaciu00f3n del Gobierno de Extremadura

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