English
French
Inhibition of the mitochondrial permeability transition pore reduces “apoptosis like” changes during cryopreservation of stallion spermatozoa.
Abstract: In order to evaluate to what extent the changes that occur during cryopreservation involve the mitochondrial permeability transition pore (PT-pore), a specific inhibitor was used during the cryopreservation process of stallion spermatozoa. Four ejaculates from each of 7 stallions were frozen using a standard protocol. Before freezing, each ejaculate was split into three subsamples. The first was supplemented with 2.5 microM bongkrekic acid (BA) and the second with 5 microM BA. The third subsample served as control. The BA significantly reduced the percentage of spermatozoa depicting active caspases after thawing, reduced the percentage of spermatozoa with increased membrane permeability, and increased the mitochondrial membrane potential of thawed sperm. Sperm motility was reduced as a result of the treatment. It is concluded that the mitochondrial pathway of apoptosis seems to be an important factor involved in the sublethal damage that equine spermatozoa experience after freezing and thawing, and that sperm motility in the equine species is largely dependent on mitochondrial ATP produced by oxidative phosphorylation.
Copyright 2010 Elsevier Inc. All rights reserved.
Publication Date: 2010-05-10 PubMed ID: 20451990DOI: 10.1016/j.theriogenology.2010.02.029Google Scholar: Lookup The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
- Journal Article
- Research Support
- Non-U.S. Gov't
- Adenosine Triphosphate (ATP)
- Animal Studies
- Apoptosis
- Biochemistry
- Cell Biology
- Cryopreservation
- Equine Diseases
- Equine Health
- Freezing Technique
- In Vitro Research
- Mitochondria
- Oxidative Phosphorylation
- Physiology
- Reproduction
- Semen Analysis
- Semen Preservation
- Spermatogenesis
- Stallion
- Veterinary Medicine
- Veterinary Research
Summary
This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.
This research investigates the impacts of using an inhibitor during the cryopreservation process of stallion spermatozoa, particularly focusing on reducing the ‘apoptosis-like’ changes. It concludes that an inhibitor can significantly reduce damage and maintain mitochondrial function, though it reduces sperm motility.
Research Process
- The research was conducted using sperm samples from seven stallions. Each stallion provided four ejaculates which were frozen using a standard protocol.
- Prior to freezing, each ejaculate was divided into three subsamples. Two of these were treated with bongkrekic acid (BA)—an inhibitor of the mitochondrial permeability transition pore (PT-pore)—at concentrations of 2.5 microM and 5 microM, respectively. The third subsample was left untreated to act as a control.
Findings
- Following thawing, the subsamples previously treated with BA showed significant reductions in the percentage of spermatozoa exhibiting active caspases—a sign of apoptotic (cell death) activity.
- The BA treatment also decreased the percentage of spermatozoa presenting increased membrane permeability—another potential indicator of cell damage.
- In addition, the mitochondrial membrane potential (a measure of a cell’s capacity to generate ATP, the primary energy currency of the cell) was higher in thawed sperm from the BA-treated subsamples, indicating the preserved functionality of the mitochondria.
- However, a downside/limitation of the BA treatment was noted: it reduced sperm motility, which is an essential characteristic for successful fertilization.
Conclusion
- The research concludes that the mitochondrial pathway of apoptosis, which is mediated by the PT-pore, appears to be a significant factor contributing to the sublethal damage that occurs in equine spermatozoa during freezing and thawing.
- Importantly, the study also indicates that equine sperm motility is largely dependent on mitochondrial ATP, which is produced through oxidative phosphorylation. This key insight could guide future research or be factored into cryopreservation protocol adjustments to minimize impairment to sperm motility.
Cite This Article
APA
Ortega Ferrusola C, Gonzu00e1lez Fernu00e1ndez L, Salazar Sandoval C, Macu00edas Garcu00eda B, Rodru00edguez Martu00ednez H, Tapia JA, Peu00f1a FJ.
(2010).
Inhibition of the mitochondrial permeability transition pore reduces “apoptosis like” changes during cryopreservation of stallion spermatozoa.
Theriogenology, 74(3), 458-465.
https://doi.org/10.1016/j.theriogenology.2010.02.029 Publication
Researcher Affiliations
- Veterinary Teaching Hospital, Laboratory of Spermatology, University of Extremadura Avd de la Universidad s/n Cu00e1ceres Spain.
MeSH Terms
- Animals
- Apoptosis
- Bongkrekic Acid / pharmacology
- Caspases / metabolism
- Cryopreservation / veterinary
- Horses
- Lipid Peroxidation
- Male
- Membrane Potential, Mitochondrial / drug effects
- Mitochondria / drug effects
- Mitochondria / physiology
- Mitochondrial Membrane Transport Proteins / antagonists & inhibitors
- Mitochondrial Permeability Transition Pore
- Permeability / drug effects
- Semen Preservation / veterinary
- Sperm Motility / drug effects
- Spermatozoa / drug effects
- Spermatozoa / enzymology
- Spermatozoa / ultrastructure
Citations
This article has been cited 19 times.- Luo X, Liang M, Huang S, Xue Q, Ren X, Li Y, Wang J, Shi D, Li X. iTRAQ-based comparative proteomics reveal an enhancing role of PRDX6 in the freezability of Mediterranean buffalo sperm.. BMC Genomics 2023 May 5;24(1):245.
- Fu Y, Dang W, He X, Xu F, Huang H. Biomolecular Pathways of Cryoinjuries in Low-Temperature Storage for Mammalian Specimens.. Bioengineering (Basel) 2022 Oct 12;9(10).
- Benko F, Mohammadi-Sangcheshmeh A, u010eurau010dka M, Luku00e1u010d N, Tvrdu00e1 E. In vitro versus cryo-induced capacitation of bovine spermatozoa, part 1: Structural, functional, and oxidative similarities and differences.. PLoS One 2022;17(10):e0276683.
- Gao S, Zhao BX, Long C, Heng N, Guo Y, Sheng XH, Wang XG, Xing K, Xiao LF, Ni HM, Wu SG, Qi XL. Natural Astaxanthin Improves Testosterone Synthesis and Sperm Mitochondrial Function in Aging Roosters.. Antioxidants (Basel) 2022 Aug 29;11(9).
- Medica AJ, Aitken RJ, Nicolson GL, Sheridan AR, Swegen A, De Iuliis GN, Gibb Z. Glycerophospholipids protect stallion spermatozoa from oxidative damage in vitro.. Reprod Fertil 2021 Jul;2(3):199-209.
- Yu00e1nez-Ortiz I, Catalu00e1n J, Mateo-Otero Y, Dordas-Perpinyu00e0 M, Gacem S, Yeste N, Bassols A, Yeste M, Miru00f3 J. Extracellular Reactive Oxygen Species (ROS) Production in Fresh Donkey Sperm Exposed to Reductive Stress, Oxidative Stress and NETosis.. Antioxidants (Basel) 2021 Aug 27;10(9).
- Lanu00e7oni R, Celeghini ECC, Giuli V, de Carvalho CPT, Zoca GB, Garcia-Oliveros LN, Batissaco L, Oliveira LZ, de Arruda RP. Coenzyme Q-10 improves preservation of mitochondrial functionality and actin structure of cryopreserved stallion sperm.. Anim Reprod 2021 Mar 31;18(1):e20200218.
- Gualtieri R, Kalthur G, Barbato V, Di Nardo M, Adiga SK, Talevi R. Mitochondrial Dysfunction and Oxidative Stress Caused by Cryopreservation in Reproductive Cells.. Antioxidants (Basel) 2021 Feb 24;10(3).
- Sotgia S, Taras A, Zinellu A, Cherchi R, Mangoni AA, Carru C, Bogliolo L. Hercynine, Ergothioneine and Redox State in Stallion's Seminal Plasma.. Antioxidants (Basel) 2020 Sep 13;9(9).
- Peris-Frau P, Soler AJ, Iniesta-Cuerda M, Martu00edn-Maestro A, Su00e1nchez-Ajofru00edn I, Medina-Chu00e1vez DA, Fernu00e1ndez-Santos MR, Garcu00eda-u00c1lvarez O, Maroto-Morales A, Montoro V, Garde JJ. Sperm Cryodamage in Ruminants: Understanding the Molecular Changes Induced by the Cryopreservation Process to Optimize Sperm Quality.. Int J Mol Sci 2020 Apr 16;21(8).
- Fernu00e1ndez-Alegre E, u00c1lvarez-Fernu00e1ndez I, Domu00ednguez JC, Casao A, Martu00ednez-Pastor F. Melatonin Non-Linearly Modulates Bull Spermatozoa Motility and Physiology in Capacitating and Non-Capacitating Conditions.. Int J Mol Sci 2020 Apr 13;21(8).
- Fang Y, Zhao C, Xiang H, Zhao X, Zhong R. Melatonin Inhibits Formation of Mitochondrial Permeability Transition Pores and Improves Oxidative Phosphorylation of Frozen-Thawed Ram Sperm.. Front Endocrinol (Lausanne) 2019;10:896.
- Villaverde AISB, Netherton J, Baker MA. From Past to Present: The Link Between Reactive Oxygen Species in Sperm and Male Infertility.. Antioxidants (Basel) 2019 Dec 3;8(12).
- Peu00f1a FJ, O'Flaherty C, Ortiz Rodru00edguez JM, Martu00edn Cano FE, Gaitskell-Phillips GL, Gil MC, Ortega Ferrusola C. Redox Regulation and Oxidative Stress: The Particular Case of the Stallion Spermatozoa.. Antioxidants (Basel) 2019 Nov 19;8(11).
- Len JS, Koh WSD, Tan SX. The roles of reactive oxygen species and antioxidants in cryopreservation.. Biosci Rep 2019 Aug 30;39(8).
- Riva NS, Ruhlmann C, Iaizzo RS, Marcial Lu00f3pez CA, Martu00ednez AG. Comparative analysis between slow freezing and ultra-rapid freezing for human sperm cryopreservation.. JBRA Assist Reprod 2018 Nov 1;22(4):331-337.
- Gibb Z, Aitken RJ. The Impact of Sperm Metabolism during In Vitro Storage: The Stallion as a Model.. Biomed Res Int 2016;2016:9380609.
- Plaza Davila M, Martin Muu00f1oz P, Tapia JA, Ortega Ferrusola C, Balao da Silva C C, Peu00f1a FJ. Inhibition of Mitochondrial Complex I Leads to Decreased Motility and Membrane Integrity Related to Increased Hydrogen Peroxide and Reduced ATP Production, while the Inhibition of Glycolysis Has Less Impact on Sperm Motility.. PLoS One 2015;10(9):e0138777.
- Chauvignu00e9 F, Boj M, Finn RN, Cerdu00e0 J. Mitochondrial aquaporin-8-mediated hydrogen peroxide transport is essential for teleost spermatozoon motility.. Sci Rep 2015 Jan 14;5:7789.
Secure Payments
Satisfaction Guarantee
We offer a 60-day guarantee to ensure you are completely satisfied.
Fast & Free Shipping
We provide fast and free shipping on all orders over $40.