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Veterinary research communications1991; 15(6); 427-435; doi: 10.1007/BF00346538

Purification of equine neutrophil lysozyme and its antibacterial activity against gram-positive and gram-negative bacteria.

Abstract: Lysozyme from equine neutrophil granulocytes was isolated in a pure form by fast performance liquid chromatography, i.e. ion-exchange chromatography and reversed-phase chromatography. The lysozyme lysed Micrococcus luteus, Bacillus subtilis and Staphylococcus lentus and was also bactericidal against the Gram-negative bacteria Escherichia coli, Klebsiella pneumoniae, Bordetella bronchiseptica, and Serratia marcescens. Staphylococcus aureus and Staphylococcus epidermidis were not lysed. The lysozyme was only very slightly bactericidal for S. epidermidis and S. aureus. Equine neutrophil lysozyme was found to be bactericidal for Gram-positive as well as for Gram-negative bacteria without further treatment. Equine and chicken egg white lysozymes were found to be immunologically related when examined using specific antisera against each of them. Both lysozymes also had very similar specific enzymatic activities against M. luteus membranes.
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Publication Date: 1991-01-01 PubMed ID: 1803722DOI: 10.1007/BF00346538Google Scholar: Lookup
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  • Journal Article
  • Research Support
  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research article focuses on the purification process of lysozyme, an antibacterial enzyme, derived from equine neutrophil granulocytes and its effective antibacterial activity against both gram-positive and gram-negative bacteria.

Purification of Lysozyme

  • The study presents a method for the purification of equine neutrophil lysozyme using fast performance liquid chromatography techniques. This involved ion-exchange chromatography and reversed-phase chromatography. These scientific processes are used to isolate the desired component, the lysozyme, in its purest form from the mixtures obtained from equine neutrophil granulocytes.

Antibacterial Activity

  • The purified lysozyme demonstrates strong antibacterial activity. It successfully lysed, or broke down, gram-positive bacteria such as Micrococcus luteus, Bacillus subtilis, and Staphylococcus lentus.
  • The lysozyme also demonstrated bactericidal effects, or bacteria-killing activity, on gram-negative bacteria strains, such as Escherichia coli, Klebsiella pneumoniae, Bordetella bronchiseptica, and Serratia marcescens.
  • However, two strains of gram-positive bacteria, Staphylococcus aureus and Staphylococcus epidermidis, were not lysed by the lysozyme, but they were very slightly affected.

Comparative Analysis

  • Equine neutrophil lysozyme and chicken egg white lysozymes were compared in the study. It was found that, healthwise, these lysozymes were related. This conclusion was drawn based on tests conducted using specific antisera against each of them.
  • Both types of lysozymes exhibited similar specific enzymatic activities against the membranes of Micrococcus luteus.

In conclusion, the study suggests that equine neutrophil lysozyme, once properly purified, offers potential as an effective antibacterial agent against a range of bacteria types with minor exceptions. Further, it is found to align closely with the behavior of lysozymes derived from chicken egg whites.

Cite This Article

APA
Pellegrini A, Waiblinger S, Von Fellenberg R. (1991). Purification of equine neutrophil lysozyme and its antibacterial activity against gram-positive and gram-negative bacteria. Vet Res Commun, 15(6), 427-435. https://doi.org/10.1007/BF00346538

Publication

Veterinary research communications
ISSN: 0165-7380
NlmUniqueID: 8100520
Country: Switzerland
Language: English
Volume: 15
Issue: 6
Pages: 427-435

Researcher Affiliations

Pellegrini, A
  • Division of Applied Veterinary Physiology, University of Zürich, Switzerland.
Waiblinger, S
    Von Fellenberg, R

      MeSH Terms

      • Animals
      • Chromatography, Ion Exchange
      • Electrophoresis, Agar Gel
      • Electrophoresis, Polyacrylamide Gel
      • Gram-Negative Bacteria / drug effects
      • Gram-Negative Bacteria / immunology
      • Gram-Positive Bacteria / drug effects
      • Gram-Positive Bacteria / immunology
      • Horses / blood
      • Horses / immunology
      • Muramidase / immunology
      • Muramidase / isolation & purification
      • Muramidase / pharmacology
      • Neutrophils / enzymology
      • Neutrophils / immunology

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      Citations

      This article has been cited 5 times.
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        doi: 10.1007/s00253-021-11716-xpubmed: 34874472google scholar: lookup
      2. Minamijima Y, Niwa H, Uchida E, Yamamoto K. Comparison of the proteomes in sera between healthy Thoroughbreds and Thoroughbreds with respiratory disease associated with transport using mass spectrometry-based proteomics. J Equine Sci 2021 Mar;32(1):11-15.
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      4. Bruhn O, Grötzinger J, Cascorbi I, Jung S. Antimicrobial peptides and proteins of the horse--insights into a well-armed organism. Vet Res 2011 Sep 2;42(1):98.
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      5. Skerlavaj B, Scocchi M, Gennaro R, Risso A, Zanetti M. Structural and functional analysis of horse cathelicidin peptides. Antimicrob Agents Chemother 2001 Mar;45(3):715-22.
        doi: 10.1128/AAC.45.3.715-722.2001pubmed: 11181349google scholar: lookup
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