Putative mRNA Biomarkers for the Eradication of Infection in an Equine Experimental Model of Septic Arthritis.
Abstract: Septic arthritis (SA) in horses has long-term health implications. The success of its resolution hinges on the implementation of early, aggressive treatment, which is often sustained over a prolonged period. Common diagnostic methods do not allow for the reliable detection of the eradication of joint infection. A potential alternative is the discovery and characterization of mRNA biomarkers. The purpose of this study was to identify potential mRNA biomarkers for the eradication of joint infection in equine SA and to compare their expression with our previously published proteomics data. In addition, the transcriptomics data were compared to the mRNA biomarker panel, SeptiCyte Lab, used to distinguish sepsis from non-septic shock in humans. A comparative transcriptomics analysis of synovial fluid from the SA joints of five horses with active infection and subsequent post-treatment eradicated infection in the same joints and five horses with non-septic synovitis was performed. Eight novel mRNA transcripts were identified that were significantly upregulated (>3-fold) in horses with active SA compared to horses post-eradication of infection after treatment and horses with non-septic synovitis. Two proteins in our proteomics data corresponded to these mRNA transcripts, but were not statistically different. The transcripts used in the SeptiCyte test were not differentially expressed in our study. Our results suggest that mRNA may be a useful source of biomarkers for the eradication of joint infection in horses and warrants further investigation.
Publication Date: 2024-07-02 PubMed ID: 39057983PubMed Central: PMC11281635DOI: 10.3390/vetsci11070299Google Scholar: Lookup
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- Journal Article
Summary
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This research study investigates potential mRNA biomarkers that could help with detecting when joint infections in horses with septic arthritis have been successfully cleared. Current common diagnostic methods are not reliable for confirming if the infection has been fully eradicated.
Research Objective and Methodology
- In this study, the researchers were looking for mRNA biomarkers that could reliably indicate the eradication of joint infection in horses with septic arthritis. The earliest and most efficient treatment of septic arthritis (SA), a serious and long-term health issue in horses, greatly enhances the chances of successful resolution. Current diagnostic techniques, however, are not fully reliable in detecting the complete elimination of infection in the joint. The exploration and understanding of mRNA biomarkers offer a potentially more reliable alternative.
- To carry out this study, a comparative transcriptomics analysis was undertaken. This involved comparing the synovial fluid from the SA joints of five horses with active infection and also post-treatment eradication in the same joints. These samples were also compared to those from five horses suffering from non-septic synovitis. The objective was to identify changes in mRNA levels in active infection, after treatment, and between septic and non-septic conditions.
Findings
- The study found eight new mRNA transcripts that were significantly upregulated (increased by more than three times) in horses with active SA as compared to those that had completed treatment, and also compared to horses with non-septic synovitis. Two proteins that corresponded to these mRNA transcripts were identified in a previous proteomics study, but they did not show statistically significant differences.
- The study also compared these findings with the SeptiCyte Lab panel, a set of mRNA biomarkers used to distinguish sepsis from non-septic shock in humans. The researchers found that the transcripts used in the SeptiCyte test were not differently expressed in their study, indicating that the SeptiCyte panel may not be directly applicable for diagnosing infection eradication in horses.
Conclusions and Further Investigation
- The results suggest that mRNA could serve as a valuable source of biomarkers to affirm the eradication of joint infection in horses. The discovery of these mRNA biomarkers could enable more reliable detection of the clearing of infections after treatment, thereby improving disease management and recovery outcomes.
- The researchers underline that while their findings are promising, further investigation is needed to confirm the validity of these markers.
Cite This Article
APA
Koziy RV, Bracamonte JL, Katselis GS, Udenze D, Hayat S, Hammond SA, Simko E.
(2024).
Putative mRNA Biomarkers for the Eradication of Infection in an Equine Experimental Model of Septic Arthritis.
Vet Sci, 11(7).
https://doi.org/10.3390/vetsci11070299 Publication
Researcher Affiliations
- Department of Veterinary Pathology, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, SK S7N 5B4, Canada.
- Department of Large Animal Clinical Sciences, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, SK S7N 5B4, Canada.
- Canadian Centre for Rural and Agricultural Health, Department of Medicine, College of Medicine, University of Saskatchewan, Saskatoon, SK S7N 2Z4, Canada.
- Next-Generation Sequencing Facility, Cancer Research Cluster, University of Saskatchewan, Saskatoon, SK S7N 5E5, Canada.
- Next-Generation Sequencing Facility, Cancer Research Cluster, University of Saskatchewan, Saskatoon, SK S7N 5E5, Canada.
- Next-Generation Sequencing Facility, Cancer Research Cluster, University of Saskatchewan, Saskatoon, SK S7N 5E5, Canada.
- Department of Veterinary Pathology, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, SK S7N 5B4, Canada.
Grant Funding
- N/A / Townsend Equine Health Research Fund
- N/A / Mark and Pat DuMont Equine Research Fund
Conflict of Interest Statement
The authors declare no conflicts of interest.
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