FREE SHIPPING over $40
OVER 9000 FIVE-STAR REVIEWS
5% OFF ALL SUBSCRIPTIONS
100% HAPPINESS GUARANTEE
Analyze Diet
0
Home / Equine Research Bank / Reprod Domest Anim / Qualitative analysis and functional classification of the ut...
Reproduction in domestic animals = Zuchthygiene2020; 55(11); 1511-1519; doi: 10.1111/rda.13800

Qualitative analysis and functional classification of the uterine proteome of mares in oestrus and dioestrus.

Abstract: Quantitative analysis of the uterine flush fluid proteome of mares in oestrus and dioestrus has been previously reported. The objectives of this study were to: a) evaluate qualitative differences in the uterine flush fluid proteome between mares in oestrus and mares in dioestrus and b) perform a functional classification of proteins either unique to each stage or common between the two stages. Uterine flush fluid samples were collected from 8 light breed mares in either oestrus (n = 5) or dioestrus (n = 3). Proteomic analysis of the samples was conducted using liquid chromatography-tandem mass spectrometry. Proteins exclusively detected in oestrus or dioestrus and those common to both stages were identified using the Scaffold software (version 4.4.8, Proteome Software Inc., Portland, OR). The identified proteins were classified into gene ontology (GO) categories (cellular component [CC], molecular function [MF] and biological process [BP]) using the PANTHER (www.pantherdb.org) classification system version 14.0. Of 172 proteins identified, 51 and 28 were exclusively detected in mares in oestrus and dioestrus, respectively, and 93 proteins were common to both stages. The most represented terms in various GO categories were similar among the three subsets of proteins. The most represented CC terms were extracellular region and cell, the most represented MF terms were catalytic activity and binding, and the most represented BP terms were metabolic process and cellular process. In conclusion, proteomic analysis of the uterine flush fluid enabled the identification of subsets of proteins unique to oestrus or dioestrus, or common to both stages. The results of this study can serve as a baseline for future research focused on finding stage-specific protein markers or evaluating differences in the uterine flush fluid proteome between normal mares and those with uterine disease.
© 2020 Wiley-VCH GmbH.
Get Full Text
Publication Date: 2020-09-15 PubMed ID: 32772405DOI: 10.1111/rda.13800Google Scholar: Lookup
The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
LinkedInCopy
  • Comparative Study
  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research article investigates the differences and similarities in the protein makeup found in a mare’s uterine fluid during two reproductive stages, oestrus and dioestrus. The aim is to catalogue these proteins for future studies related to equine uterine health and potential disease markers.

Research Objectives

The main objectives of this study were to:

  • Evaluate qualitative differences in the uterine flush fluid proteome (the set of proteins found in the fluid) between mares in oestrus (a stage when mares are sexually receptive) and mares in dioestrus (the stage immediately following oestrus).
  • Classify the proteins unique to each stage and those common to both stages.

Methodology

Eight light breed mare uterine fluid samples were collected and analyzed using liquid chromatography-tandem mass spectrometry, which is a sophisticated technique for identifying and quantifying proteins within a sample. The researchers used software to identify proteins that were exclusively present in mares in either the oestrus or the dioestrus stage, as well as proteins that were common in both stages.

Findings

The researchers identified:

  • 172 total proteins in the uterine fluid samples.
  • 51 proteins were unique to mares in the oestrus stage.
  • 28 proteins were unique to mares in the dioestrus stage.
  • 93 proteins were common to both stages.

Importantly, the proteins were further classified into gene ontology (GO) categories involving cellular components, molecular function and biological processes. The classifications provided patterns in the subsets of proteins, suggesting their role in the horse’s reproductive cycle.

Conclusion

The findings in this study have offered a significant foundation for future research. Identifying stage-specific protein markers and evaluating differences in the uterine flush fluid proteome between normal mares and those with uterine disease could greatly aid in the understanding and treatment of equine reproductive health.

Cite This Article

APA
Khan FA, Diel de Amorim M, Chenier TS. (2020). Qualitative analysis and functional classification of the uterine proteome of mares in oestrus and dioestrus. Reprod Domest Anim, 55(11), 1511-1519. https://doi.org/10.1111/rda.13800

Publication

Reproduction in domestic animals = Zuchthygiene
ISSN: 1439-0531
NlmUniqueID: 9015668
Country: Germany
Language: English
Volume: 55
Issue: 11
Pages: 1511-1519

Researcher Affiliations

Khan, Firdous A
  • Department of Population Medicine, Ontario Veterinary College, University of Guelph, Guelph, ON, Canada.
  • Department of Large Animal Medicine and Surgery, School of Veterinary Medicine, St. George's University, St. George's, Grenada.
Diel de Amorim, Mariana
  • Department of Population Medicine, Ontario Veterinary College, University of Guelph, Guelph, ON, Canada.
Chenier, Tracey S
  • Department of Population Medicine, Ontario Veterinary College, University of Guelph, Guelph, ON, Canada.

MeSH Terms

  • Animals
  • Diestrus / metabolism
  • Estrus / metabolism
  • Female
  • Horses
  • Proteome / analysis
  • Uterus / metabolism

Grant Funding

  • RGPIN-2009-155433 / Natural Sciences and Engineering Research Council of Canada
  • Ontario Ministry of Agriculture, Food and Rural Affairs (OMAFRA)

References

This article includes 31 references
  1. Afonso S, Romagnano L, Babiarz B. The expression and function of cystatin C and cathepsin B and cathepsin L during mouse embryo implantation and placentation. Development 124, 3415-3425.
  2. Anderson DJ, Abbott AF, Jack RM. The role of complement component C3b and its receptors in sperm-oocyte interaction. Proceedings of the National Academy of Sciences United States of America 90, 10051-10055.
    doi: 10.1073/pnas.90.21.10051google scholar: lookup
  3. Arlas TR, Wolf CA, Petrucci BP, Estanislau JF, Gregory RM, Jobim MI, Mattos RC. Proteomics of endometrial fluid after dexamethasone treatment in mares susceptible to endometritis. Theriogenology 84, 617-623.
    doi: 10.1016/j.theriogenology.2015.04.019google scholar: lookup
  4. Badinga L, Michel FJ, Fields MJ, Sharp DC, Simmen RC. Pregnancy-associated endometrial expression of antileukoproteinase gene is correlated with epitheliochorial placentation. Molecular, Reproduction and Development 38, 357-363.
    doi: 10.1002/mrd.1080380402google scholar: lookup
  5. Badinga L, Michel FJ, Simmen RC. Uterine-associated serine protease inhibitors stimulate deoxyribonucleic acid synthesis in porcine endometrial glandular epithelial cells of pregnancy. Biology of Reproduction 61, 380-387.
    doi: 10.1095/biolreprod61.2.380google scholar: lookup
  6. Bastos HBA, Martinez MN, Camozzato GC, Estradé MJ, Barros E, Vital CE, Mattos RC. Proteomic profile of histotroph during early embryo development in mares. Theriogenology 125, 224-235.
    doi: 10.1016/j.theriogenology.2018.11.002google scholar: lookup
  7. Bollwein H, Weber F, Kolberg B, Stolla R. Uterine and ovarian blood flow during the estrous cycle in mares. Theriogenology 57, 2129-2138.
    doi: 10.1016/s0093-691x(02)00703-3google scholar: lookup
  8. Diel de Amorim M, Khan FA, Chenier TS, Scholtz EL, Hayes MA. Analysis of the uterine flush fluid proteome of healthy mares and mares with endometritis or fibrotic endometrial degeneration. Reproduction, Fertility and Development 32, 572-581.
    doi: 10.1071/rd19085google scholar: lookup
  9. Elliott RM, Lloyd RE, Fazeli A, Sostaric E, Georgiou AS, Satake N, Holt WV. Effects of HSPA8, an evolutionarily conserved oviductal protein, on boar and bull spermatozoa. Reproduction 137, 191-203.
    doi: 10.1530/rep-08-0298google scholar: lookup
  10. Engle CC, Foley CW. Certain physiochemical properties of uterine tubal fluid, follicular fluid, and blood plasma in the mare. American Journal of Veterinary Research 36, 149-154.
  11. Fabryova K, Simon M. Function of the cell surface molecules (CD molecules) in the reproduction processes. General Physiology and Biophysics 28, 1-7.
    doi: 10.4149/gpb_2009_01_1google scholar: lookup
  12. Forde N, McGettigan PA, Mehta JP, O'Hara L, Mamo S, Bazer FW, Lonergan P. Proteomic analysis of uterine fluid during the pre-implantation period of pregnancy in cattle. Reproduction 147, 575-587.
    doi: 10.1530/rep-13-0010google scholar: lookup
  13. Hayes MA, Quinn BA, Lillie BN, Côté O, Bienzle D, Waelchli RO, Betteridge KJ. Changes in various endometrial proteins during cloprostenol-induced failure of early pregnancy in mares. Animal Reproduction 9, 723-741.
  14. Jalali BM, Bogacki M, Dietrich M, Likszo P, Wasielak M. Proteomic analysis of porcine endometrial tissue during peri-implantation period reveals altered protein abundance. Journal of Proteomics 125, 76-88.
    doi: 10.1016/j.jprot.2015.05.003google scholar: lookup
  15. Jokimaa V, Oksjoki S, Kujari H, Vuorio E, Anttila L. Expression patterns of cathepsins B, H, K, L and S in the human endometrium. Molecular Human Reproduction 7, 73-78.
    doi: 10.1093/molehr/7.1.73google scholar: lookup
  16. Khan FA, Chenier TS. Uterine flush fluid proteome of mares in estrus and diestrus: Qualitative differences and functional classification. Clinical Theriogenology 11, 529 (Abstract).
  17. Maloney SE, Khan FA, Chenier TS, Diel de Amorim M, Anthony Hayes M, Scholtz EL. A comparison of the uterine proteome of mares in oestrus and dioestrus. Reproduction in Domestic Animals 54, 473-479.
    doi: 10.1111/rda.13375google scholar: lookup
  18. Mi H, Muruganujan A, Huang X, Ebert D, Mills C, Guo X, Thomas PD. Protocol Update for large-scale genome and gene function analysis with the PANTHER classification system (v.14.0). Nature Protocols 14, 703-721.
    doi: 10.1038/s41596-019-0128-8google scholar: lookup
  19. Muthukumar S, Rajkumar R, Karthikeyan K, Liao CC, Singh D, Akbarsha MA, Archunan G. Buffalo cervico-vaginal fluid proteomics with special reference to estrous cycle: Heat shock protein (Hsp)-70 appears to be an estrus indicator. Biology of Reproduction 90(97), 1-8.
    doi: 10.1095/biolreprod.113.113852google scholar: lookup
  20. Nicolás CM, Accogli G, Douet C, Magistrini M, Vern YL, Sausset A, Goudet G. Highlight of new agents inducing capacitation-related changes in stallion spermatozoa. Journal of Reproductive Biology and Endocrinology 2, 61-70.
    doi: 10.15454/1.5420939363436812e12google scholar: lookup
  21. SankarGanesh D, Ramachandran R, Suriyakalaa U, Ramkumar A, Achiraman S, Archunan G. Heat shock protein(s) may serve as estrus indicators in animals: A conceptual hypothesis. Medical Hypotheses 117, 47-49.
    doi: 10.1016/j.mehy.2018.06.003google scholar: lookup
  22. Scudamore CL, Pemberton AD, Miller HR, McDonnell AM, Thomson SR, Dawson A, Watson ED. Measurement by ELISA of equine alpha-1-proteinase inhibitor in uterine flushings from mares. Research in Veterinary Science 57, 45-52.
    doi: 10.1016/0034-5288(94)90080-9google scholar: lookup
  23. Smits K, Willems S, Van Steendam K, Van De Velde M, De Lange V, Ververs C, Van Soom A. Proteins involved in embryo-maternal interaction around the signalling of maternal recognition of pregnancy in the horse. Scientific Reports 8, 5249.
    doi: 10.1038/s41598-018-23537-6google scholar: lookup
  24. Soleilhavoup C, Riou C, Tsikis G, Labas V, Harichaux G, Kohnke P, Druart X. Proteomes of the female genital tract during the oestrous cycle. Molecular and Cellular Proteomics 15, 93-108.
    doi: 10.1074/mcp.m115.052332google scholar: lookup
  25. Song G, Spencer TE, Bazer FW. Cathepsins in the ovine uterus: Regulation by pregnancy, progesterone, and interferon tau. Endocrinology 146, 4825-4833.
    doi: 10.1210/en.2005-0768google scholar: lookup
  26. Steffl M, Telgen L, Schweiger M, Amselgruber WM. Estrous cycle-dependent activity of neutrophils in the porcine endometrium: Possible involvement of heat shock protein 27 and lactoferrin. Animal Reproduction Science 121, 159-166.
    doi: 10.1016/j.anireprosci.2010.05.018google scholar: lookup
  27. Tunon AM, Rodriguez-Martinez H, Haglund A, Albihn A, Magnusson U, Einarsson S. Ultrastructure of the secretory endometrium during estrus in young maiden and foaled mares. Equine Veterinary Journal 27, 382-388.
    doi: 10.1111/j.2042-3306.1995.tb04074.xgoogle scholar: lookup
  28. Varner DD, Forrest DW, Fuentes F, Taylor TS, Hooper RN, Brinsko SP, Blanchard TL. Measurements of glycosaminoglycans in follicular, oviductal and uterine fluids of mares. Journal of Reproduction and Fertility Supplement 44, 297-306.
  29. Wolf CA, Maslchitzky E, Gregory RM, Jobim MIM, Mattos RC. Effect of corticotherapy on proteomics of endometrial fluid from mares susceptible to persistent postbreeding endometritis. Theriogenology 77, 1351-1359.
    doi: 10.1016/j.theriogenology.2011.10.042google scholar: lookup
  30. Zavy MT, Vernon MW, Asquith RL, Bazer FW, Sharp DC. Effect of exogenous gonadal steroids and pregnancy on uterine luminal prostaglandin F in mares. Prostaglandins 27, 311-320.
    doi: 10.1016/0090-6980(84)90082-0google scholar: lookup
  31. Zavy MT, Vernon MW, Sharp DCI, Bazer FW. Endocrine aspects of early pregnancy in pony mares: A comparison of uterine luminal and peripheral plasma levels of steroids during the estrous cycle and early pregnancy. Endocrinology 115, 214-219.
    doi: 10.1210/endo-115-1-214google scholar: lookup

Citations

This article has been cited 1 times.
  1. Holmes CM, Babasyan S, Wagner B. Neonatal and maternal upregulation of antileukoproteinase in horses. Front Immunol 2024;15:1395030.
    doi: 10.3389/fimmu.2024.1395030pubmed: 38736885google scholar: lookup
Subscribe to our newsletter
Join 99,357 horse owners like you who receive our email updates on horse health and nutrition.