Quantification of antigen-specific antibody concentrations in tracheal lavage fluid of horses with summer pasture-associated obstructive pulmonary disease.
Abstract: To determine whether horses with summer pasture-associated obstructive pulmonary disease (SPAOPD) have increased concentrations of antigen-specific IgG and IgE in tracheal lavage fluid, compared with values in clinically normal horses. Methods: 8 horses (6 females, 2 geldings; 6 Quarter Horses, 2 Appaloosas), 14 to 23 years old and with previous diagnosis of SPAOPD, served as the principal group; 8 horses (2 females, 6 geldings; 1 Quarter Horse, 7 Thoroughbreds), 6 to 9 years old, with no evidence of respiratory tract disease, served as the control group. Methods: Data were collected twice during a 1-year period: when all SPAOPD-affected horses were manifesting clinical signs of disease (July), and when all SPAOPD-affected horses appeared clinically normal (February). On each occasion, clinical evaluations were performed and blood and tracheal lavage fluid samples were collected. Transtracheal lavage supernatant was evaluated for mold antigen-specific IgG and IgE concentrations. Results: Median IgE relative antibody unit (RAU) values were significantly higher in control, compared with principal, horses. The SPAOPD-affected horses had increased concentrations of specific IgG for only 1 antigen, during winter sample collection. Conclusions: Antigen-specific IgG and IgE RAU values were not increased in SPAOPD-affected horses when these horses were manifesting clinical signs of disease.
Publication Date: 1997-12-24 PubMed ID: 9401689
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- Journal Article
- Research Support
- Non-U.S. Gov't
- Research Support
- U.S. Gov't
- Non-P.H.S.
- Antibodies
- Antigen
- Asthma
- Bronchoalveolar Lavage
- Chronic Obstructive Pulmonary Disease
- Clinical Pathology
- Clinical Signs
- Clinical Study
- Diagnosis
- Disease Diagnosis
- Disease Etiology
- Equine Diseases
- Equine Health
- Horses
- Immunoglobulin E
- Immunoglobulin G
- Recurrent Airway Obstruction
- Respiratory Disease
- Trachea
- Veterinary Medicine
- Veterinary Research
Summary
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The research investigates the concentrations of antigen-specific antibodies, IgG and IgE, in the tracheal fluid of horses with a condition called summer pasture-associated obstructive pulmonary disease (SPAOPD), in comparison with healthy, control horses. The results indicate that increased levels of these antibodies were not found in SPAOPD-affected horses during periods of disease manifestation.
Study Participants
- The research consisted of two groups of horses; one group constituted of horses diagnosed with SPAOPD (the principal group) and the other group made up of clinically healthy horses (control group).
- The principal group was comprised of eight horses, aged between 14 to 23 years, and they included six females and two geldings from Quarter Horse and Appaloosa breeds.
- The control group also consisted of eight horses, aged from 6 to 9 years, and included two females and six geldings mostly from the Thoroughbred breed, with one being a Quarter Horse.
Methodology
- Data were collected at two separate points during a year, specifically in July (when all SPAOPD-affected horses were manifesting symptoms) and in February (when all SPAOPD-affected horses appeared clinically normal).
- During each data collection phase, clinical evaluations were conducted, and blood and tracheal lavage fluid samples were collected from all participating horses.
- The collected tracheal lavage samples were analyzed for the concentrations of mold antigen-specific IgG and IgE antibodies.
Results
- The findings revealed that median IgE relative antibody unit (RAU) values were significantly higher in the control group compared to the principal (SPAOPD-affected) group.
- The SPAOPD-affected horses had increased concentrations of specific IgG for only one antigen, which was observed during the winter sample collection.
Conclusions
- It was concluded that the levels of antigen-specific IgG and IgE RAUs were not increased in SPAOPD-affected horses during periods when these horses were showing clinical signs of the disease.
- This suggests that increased concentrations of these antibodies may not be a reliable indicator or biomarker for exacerbations of SPAOPD.
Cite This Article
APA
Seahorn TL, Beadle RE, McGorum BC, Marley CL.
(1997).
Quantification of antigen-specific antibody concentrations in tracheal lavage fluid of horses with summer pasture-associated obstructive pulmonary disease.
Am J Vet Res, 58(12), 1408-1411.
Publication
Researcher Affiliations
- Department of Veterinary Clinical Sciences, School of Veterinary Medicine, Louisiana State University, Baton Rouge 70803, USA.
MeSH Terms
- Aging / blood
- Aging / immunology
- Animals
- Antibodies / analysis
- Antibodies / blood
- Antibodies / immunology
- Antibody Specificity
- Female
- Horse Diseases / blood
- Horse Diseases / immunology
- Horses
- Immunoglobulin E / analysis
- Immunoglobulin E / blood
- Immunoglobulin E / immunology
- Immunoglobulin G / analysis
- Immunoglobulin G / blood
- Immunoglobulin G / immunology
- Lung Diseases, Obstructive / blood
- Lung Diseases, Obstructive / immunology
- Lung Diseases, Obstructive / veterinary
- Male
- Seasons
- Therapeutic Irrigation / veterinary
- Trachea / immunology
- Urea / blood
Citations
This article has been cited 5 times.- Bright LA, Dittmar W, Nanduri B, McCarthy FM, Mujahid N, Costa LR, Burgess SC, Swiderski CE. Modeling the pasture-associated severe equine asthma bronchoalveolar lavage fluid proteome identifies molecular events mediating neutrophilic airway inflammation. Vet Med (Auckl) 2019;10:43-63.
- Venugopal CS, Holmes EP, Polikepahad S, Laborde S, Kearney M, Moore RM. Neurokinin receptors in recurrent airway obstruction: a comparative study of affected and unaffected horses. Can J Vet Res 2009 Jan;73(1):25-33.
- Polikepahad S, Haque M, Francis J, Moore RM, Venugopal CS. Characterization of endothelin receptors in the peripheral lung tissues of horses unaffected and affected with recurrent airway obstruction. Can J Vet Res 2008 Jul;72(4):340-9.
- Venugopal CS, Polikepahad S, Holmes EP, Heuvel JV, Leas TL, Moore RM. Endothelin receptor alterations in equine airway hyperreactivity. Can J Vet Res 2006 Jan;70(1):50-7.
- Venugopalan CS, Beadle RE, Seahorn TL, Holmes EP. Responses of guinea-pig lung parenchymal strips to tracheobronchial lavage fluid from horses affected with summer pasture-associated obstructive pulmonary disease. Vet Res Commun 1998 Nov;22(7):493-503.
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