Quantification of histamine in plasma and pulmonary fluids from horses with chronic obstructive pulmonary disease, before and after ‘natural (hay and straw) challenges’.
Abstract: A commercial radioimmunoassay kit was used to quantify histamine concentrations of plasma, bronchoalveolar lavage fluid (BALF) and pulmonary epithelial lining fluid (PELF) of normal horses and horses with chronic obstructive pulmonary disease (COPD), before and after 'natural (hay and straw) challenge' (NC). There were no significant changes in the concentrations of histamine in plasma or BALF at 0.5 or 5 h after NC, but the PELF histamine concentration of COPD affected horses was significantly increased at 5 h, but not at 0.5 h, following NC. As the histamine concentrations of whole BALF lysates were significantly correlated with the numbers of metachromatically staining cells, presumed to be mast cells and/or basophils, these findings support involvement of a late phase, IgE mediated, hypersensitivity reaction in the pathogenesis of equine COPD.
Publication Date: 1993-04-01 PubMed ID: 8506613DOI: 10.1016/0165-2427(93)90021-uGoogle Scholar: Lookup
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- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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The research article explores how histamine levels in various bodily fluids of horses with Chronic Obstructive Pulmonary Disease (COPD) change before and after exposure to natural elements like hay and straw.
Research Methodology
- The study involved quantifying histamine concentrations in three different bodily fluids of horses – plasma, bronchoalveolar lavage fluid (BALF), and pulmonary epithelial lining fluid (PELF).
- The samples were collected from both healthy horses and those affected by Chronic Obstructive Pulmonary Disease (COPD).
- Natural challenges (NC) – exposure to hay and straw – were given to the horses, and the histamine concentrations were measured before and after such exposure.
- A commercial radioimmunoassay kit was used for the quantification of histamine.
Findings and Interpretation
- Post natural challenge, no significant changes were noted in the histamine concentrations of plasma or BALF at 0.5 or 5 hours.
- However, the histamine concentrations in PELF of COPD-affected horses showed a significant increase 5 hours post the challenge but not at 0.5 hours.
- The study discovered that the concentrations of histamine in the BALF lysates (cellular material) were significantly correlated with the numbers of ‘metachromatically staining cells’. These are generally assumed to be mast cells and/or basophils.
- This finding indicates the possible involvement of a late-phase, Immunoglobulin E (IgE) mediated hypersensitivity reaction in the pathogenesis (origin and development) of COPD in horses.
Significance of the Study
- These findings enhance our understanding of how natural challenges influence histamine levels in horses, particularly those with COPD.
- The insights obtained could contribute to the development of new diagnostic and therapeutic strategies for managing equine COPD.
- The study has potential implications for human healthcare too, given the similar histamine-mediated allergic responses in humans and horses.
Cite This Article
APA
McGorum BC, Dixon PM, Halliwell RE.
(1993).
Quantification of histamine in plasma and pulmonary fluids from horses with chronic obstructive pulmonary disease, before and after ‘natural (hay and straw) challenges’.
Vet Immunol Immunopathol, 36(3), 223-237.
https://doi.org/10.1016/0165-2427(93)90021-u Publication
Researcher Affiliations
- Department of Veterinary Clinical Studies, Veterinary Field Station, Easter Bush, Roslin, UK.
MeSH Terms
- Allergens / immunology
- Animals
- Basophils / physiology
- Bronchoalveolar Lavage Fluid / immunology
- Bronchoalveolar Lavage Fluid / pathology
- Cell Count / veterinary
- Epithelium / chemistry
- Epithelium / immunology
- Epithelium / pathology
- Histamine / analysis
- Histamine / blood
- Horse Diseases / immunology
- Horse Diseases / pathology
- Horses
- Hypersensitivity, Immediate / immunology
- Hypersensitivity, Immediate / pathology
- Leukocyte Count / veterinary
- Lung / chemistry
- Lung / immunology
- Lung / pathology
- Lung Diseases, Obstructive / immunology
- Lung Diseases, Obstructive / pathology
- Lung Diseases, Obstructive / veterinary
- Mast Cells / physiology
- Poaceae / immunology
- Radioimmunoassay / veterinary
- Time Factors
Citations
This article has been cited 7 times.- Lo Feudo CM, Stucchi L, Alberti E, Conturba B, Zucca E, Ferrucci F. Intradermal Testing Results in Horses Affected by Mild-Moderate and Severe Equine Asthma. Animals (Basel) 2021 Jul 13;11(7).
- Slowikowska M, Bajzert J, Miller J, Stefaniak T, Niedzwiedz A. The Dynamics of Circulating Immune Complexes in Horses with Severe Equine Asthma. Animals (Basel) 2021 Apr 2;11(4).
- White SJ, Moore-Colyer M, Marti E, Hannant D, Gerber V, Coüetil L, Richard EA, Alcocer M. Antigen array for serological diagnosis and novel allergen identification in severe equine asthma. Sci Rep 2019 Oct 23;9(1):15170.
- White S, Moore-Colyer M, Marti E, Coüetil L, Hannant D, Richard EA, Alcocer M. Development of a comprehensive protein microarray for immunoglobulin E profiling in horses with severe asthma. J Vet Intern Med 2019 Sep;33(5):2327-2335.
- Einhorn L, Hofstetter G, Brandt S, Hainisch EK, Fukuda I, Kusano K, Scheynius A, Mittermann I, Resch-Marat Y, Vrtala S, Valenta R, Marti E, Rhyner C, Crameri R, Satoh R, Teshima R, Tanaka A, Sato H, Matsuda H, Pali-Schöll I, Jensen-Jarolim E. Molecular allergen profiling in horses by microarray reveals Fag e 2 from buckwheat as a frequent sensitizer. Allergy 2018 Jul;73(7):1436-1446.
- Hare JE, Viel L, Conlon PD, Marshall JS. Evaluation of an in vitro degranulation challenge procedure for equine pulmonary mast cells. Can J Vet Res 1998 Apr;62(2):133-9.
- Jentsch MC, Lübke S, Schrödl W, Volke D, Krizsan A, Hoffmann R, Kaiser-Thom S, Gerber V, Marti E, Wagner B, Schnabel CL. Immunoproteomics enable broad identification of new Aspergillus fumigatus antigens in severe equine asthma. Front Immunol 2024;15:1347164.
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