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Quantification of hypoglycin A in serum using aTRAQ(®) assay.
Abstract: Hypoglycin A has been recently identified has the causal agent of atypical myopathy (AM) in horses. Its identification and quantification in equine's biological fluids is thus a major concern to confirm maple poisoning and to provide insight into the poorly understood mechanism of hypoglycin A intoxication. Methods: Quantification of hypoglycin A has been achieved with the aTRAQ kit for amino acid analysis of physiological fluids (AB Sciex). Acquisition method on mass spectrometer has been updated to record the hypoglycin A specific MRM transition. Results: Outlined accuracy profiles demonstrated very reliable data. A good linearity was observed from 0.09 to 50μmol/L and precision was very good with coefficient of variation below 8%. Fifty-five samples collected from 25 confirmed AM horses revealed significant hypoglycin A concentrations, while toxin was not found in serum of 8 control animals. Conclusions: The described aTRAQ variant method has been analytically and clinically validated. The reliability of our approach is thus demonstrated into the workup of atypical myopathy.
Copyright © 2015 Elsevier B.V. All rights reserved.
Publication Date: 2015-06-10 PubMed ID: 26094208DOI: 10.1016/j.jchromb.2015.06.004Google Scholar: Lookup The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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The research paper presents the development of an efficient method for quantifying hypoglycin A, a harmful compound causing Atypical Myopathy in horses, in biological fluids using an aTRAQ kit.
Research Objectives and Methods
- The primary objective of the study was to devise an effective way of identifying and quantifying hypoglycin A in horses’ bodily fluids. This hazardous compound is known to lead to Atypical Myopathy (AM), a medical condition affecting horses.
- This identification and quantification are crucial because understanding the mechanism of hypoglycin A intoxication can help confirm cases of maple poisoning in horses. Maple poisoning is another name for Atypical Myopathy, referring to the toxic compound found in the seeds of certain types of maple trees consumed by horses.
- To accomplish this objective, researchers relied on an aTRAQ kit for the amino acid analysis of physiological fluids, specifically developed by AB Sciex.
- The primary method of data collection was Mass Spectrometry, which had been adjusted to record the specific MRM (Multiple Reaction Monitoring) transition for hypoglycin A.
Results and Validations
- The analytical accuracy profiles generated by the research study yielded highly reliable data. There was a notable linearity observed from 0.09 to 50μmol/L, with excellent precision, as shown by a coefficient of variation below 8%.
- During the research, fifty-five samples were procured from twenty-five horses confirmed to have AM. These samples showed a significant concentration of hypoglycin A. No trace of the toxin was found in the serum of eight control animals.
- The results helped validate the aTRAQ method’s analytical and clinical value and confirmed its reliability as a part of the workup for identifying atypical myopathy.
Conclusions
- The study effectively demonstrated an efficient method of identifying and quantifying hypoglycin A in horses’ bodily fluids, contributing to the body of knowledge on Atypical Myopathy.
- The application of the aTRAQ kit can help in confirming cases of maple poisoning in horses and further our understanding of hypoglycin A intoxication’s mechanism.
Cite This Article
APA
Boemer F, Deberg M, Schoos R, Baise E, Amory H, Gault G, Carlier J, Gaillard Y, Marcillaud-Pitel C, Votion D.
(2015).
Quantification of hypoglycin A in serum using aTRAQ(®) assay.
J Chromatogr B Analyt Technol Biomed Life Sci, 997, 75-80.
https://doi.org/10.1016/j.jchromb.2015.06.004 Publication
Researcher Affiliations
- Biochemical Genetics Laboratory, Human Genetics, CHU Liege, University of Liege, Belgium. Electronic address: f.boemer@chu.ulg.ac.be.
- Biochemical Genetics Laboratory, Human Genetics, CHU Liege, University of Liege, Belgium.
- Biochemical Genetics Laboratory, Human Genetics, CHU Liege, University of Liege, Belgium.
- Department of animal Productions: Biostatistics, Economy and animal selection, Fundamental and Applied Research for Animals & Health (FARAH), Faculty of Veterinary Medicine, University of Liege, Belgium.
- Equine Pole, Fundamental and Applied Research for Animals & Health (FARAH), Faculty of Veterinary Medicine, University of Liege, Belgium.
- Usc 1233 Ediss, VETAGROSUP, Ecole nationale vétérinaire de Lyon, France.
- LAT LUMTOX, La Voulte-sur-Rhône, France.
- LAT LUMTOX, La Voulte-sur-Rhône, France.
- Réseau d'EpidémioSurveillance en Pathologie Equine (RESPE), Caen, France.
- Equine Pole, Fundamental and Applied Research for Animals & Health (FARAH), Faculty of Veterinary Medicine, University of Liege, Belgium.
MeSH Terms
- Animals
- Horse Diseases / blood
- Horses
- Hypoglycins / blood
- Isotope Labeling
- Linear Models
- Mass Spectrometry / methods
- Muscular Diseases / blood
- Reproducibility of Results
- Sensitivity and Specificity
Citations
This article has been cited 9 times.- François AC, Taminiau B, Renaud B, Gonza-Quito IE, Massey C, Hyde C, Piercy RJ, Douny C, Scippo ML, Daube G, Gustin P, Delcenserie V, Votion DM. In Vitro Investigation of Equine Gut Microbiota Alterations During Hypoglycin A Exposure. Animals (Basel) 2025 Nov 19;15(22).
- François AC, Cesarini C, Taminiau B, Renaud B, Kruse CJ, Boemer F, van Loon G, Palmers K, Daube G, Wouters CP, Lecoq L, Gustin P, Votion DM. Unravelling Faecal Microbiota Variations in Equine Atypical Myopathy: Correlation with Blood Markers and Contribution of Microbiome. Animals (Basel) 2025 Jan 26;15(3).
- Wouters CP, Klein B, Price N, Boemer F, Voz ML, Votion DM. A Zebrafish Embryo Model to Screen Potential Therapeutic Compounds in Sapindaceae Poisoning. Molecules 2024 Oct 19;29(20).
- Kruse CJ, Dieu M, Renaud B, François AC, Stern D, Demazy C, Burteau S, Boemer F, Art T, Renard P, Votion DM. New Pathophysiological Insights from Serum Proteome Profiling in Equine Atypical Myopathy. ACS Omega 2024 Feb 13;9(6):6505-6526.
- Renaud B, Kruse CJ, François AC, Grund L, Bunert C, Brisson L, Boemer F, Gault G, Ghislain B, Petitjean T, Gustin P, Votion DM. Acer pseudoplatanus: A Potential Risk of Poisoning for Several Herbivore Species. Toxins (Basel) 2022 Jul 26;14(8).
- Renaud B, François AC, Boemer F, Kruse C, Stern D, Piot A, Petitjean T, Gustin P, Votion DM. Grazing Mares on Pasture with Sycamore Maples: A Potential Threat to Suckling Foals and Food Safety through Milk Contamination. Animals (Basel) 2021 Jan 5;11(1).
- Sanford AA, Isenberg SL, Carter MD, Mojica MA, Mathews TP, Laughlin S, Thomas JD, Pirkle JL, Johnson RC. Quantification of hypoglycin A and methylenecyclopropylglycine in human plasma by HPLC-MS/MS. J Chromatogr B Analyt Technol Biomed Life Sci 2018 Sep 15;1095:112-118.
- Bunert C, Langer S, Votion DM, Boemer F, Müller A, Ternes K, Liesegang A. Atypical myopathy in Père David's deer (Elaphurus davidianus) associated with ingestion of hypoglycin A. J Anim Sci 2018 Jul 28;96(8):3537-3547.
- Boemer F, Detilleux J, Cello C, Amory H, Marcillaud-Pitel C, Richard E, van Galen G, van Loon G, Lefère L, Votion DM. Acylcarnitines profile best predicts survival in horses with atypical myopathy. PLoS One 2017;12(8):e0182761.
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