Rapid detection of group C streptococci from animals by latex agglutination.
Abstract: A group C latex agglutination reagent, included as the negative control in the PathoDx Strep A latex agglutination test (Diagnostic Products Corp., Los Angeles, Calif), was compared with culture for rapid detection of group C streptococci (Streptococcus equi, S. equisimilis, S. zooepidemicus, and S. dysgalactiae) from swabs of veterinary specimens. The overall sensitivity of the latex test was 78%, and specificity was 97.6%. Only 25% of S. dysgalactiae isolates were detected, thereby accounting for the relatively low sensitivity. Ninety-three percent of the group C streptococci other than S. dysgalactiae were isolated from horses. When the latex test was evaluated for detection of group C streptococci other than S. dysgalactiae from horses, the sensitivity and specificity were 95.3 and 100%, respectively. We found the group C latex agglutination test to be a rapid and accurate method for the detection of the major pathogenic group C streptococci from swabs of equine specimens.
Publication Date: 1989-02-01 PubMed ID: 2915025PubMed Central: PMC267298DOI: 10.1128/jcm.27.2.309-312.1989Google Scholar: Lookup
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- Journal Article
Summary
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This research article focuses on the effectiveness of using a Group C latex agglutination test as a quick method to detect Group C streptococci, primarily in horses. The method was shown to be sensitive and highly specific apart from lower detection rates for S. dysgalactiae.
Research Objectives and Methods
- The goal of the research was to assess the effectiveness of a group C latex agglutination reagent in quickly detecting group C streptococci in veterinary specimens.
- The latex reagent, deployed as the negative control in the PathoDx Strep A latex agglutination test, was used for this investigation.
- The evaluation involved comparing results obtained from the latex test with culture results obtained from swab samples.
Findings of the Study
- The latex test showed an overall sensitivity of 78% and a specificity of 97.6%, signifying its effectiveness not only in detecting true positives but also in excluding true negatives.
- The test, however, had limited success in detecting S. dysgalactiae – only 25% of such cases were detected, which led to a lower overall sensitivity.
- A large majority (93%) of the group C streptococci other than S. dysgalactiae were found in horses.
- When the latex test was specifically used for detecting group C streptococci excluding S. dysgalactiae in horses, the sensitivity increased to 95.3% and specificity reached 100%. This showed the test was highly effective when used specifically for equine specimens excluding S. dysgalactiae.
Conclusion
- The researchers concluded that the group C latex agglutination test is a fast and precise method for detecting major pathogenic group C streptococci from equine specimen swabs.
- The test is, however, less effective in detecting S. dysgalactiae, and this area may require further investigation for improvements.
Cite This Article
APA
Inzana TJ, Iritani B.
(1989).
Rapid detection of group C streptococci from animals by latex agglutination.
J Clin Microbiol, 27(2), 309-312.
https://doi.org/10.1128/jcm.27.2.309-312.1989 Publication
Researcher Affiliations
- Department of Veterinary Microbiology-Pathology, Washington State University, Pullman 99164-7040.
MeSH Terms
- Animals
- Antigens, Bacterial / analysis
- Diagnostic Errors
- Horses / microbiology
- Latex Fixation Tests / veterinary
- Streptococcus / isolation & purification
References
This article includes 7 references
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- Friedman CA, Wender DF, Rawson JE. Rapid diagnosis of group B streptococcal infection utilizing a commercially available latex agglutination assay.. Pediatrics 1984 Jan;73(1):27-30.
- Bannister MF, Benson CE, Sweeney CR. Rapid species identification of group C streptococci isolated from horses.. J Clin Microbiol 1985 Apr;21(4):524-6.
- Slifkin M, Gil GM. Evaluation of the Culturette Brand Ten-Minute Group A Strep ID technique.. J Clin Microbiol 1984 Jul;20(1):12-4.
- Facklam RR. Specificity study of kits for detection of group A streptococci directly from throat swabs.. J Clin Microbiol 1987 Mar;25(3):504-8.
- Wald ER, Dashefsky B, Green M, Harger J, Parise M, Korey C, Byers C. Rapid detection of group B streptococci directly from vaginal swabs.. J Clin Microbiol 1987 Mar;25(3):573-4.
- Granato PA, Giachetti-Powers LM, Murfitt KS, King D. Comparative evaluation of PathoDx Strep A test and culture for the detection of group A streptococci in pharyngeal specimens.. Diagn Microbiol Infect Dis 1986 Nov;5(4):293-8.
Citations
This article has been cited 1 times.- Hayden GF, Turner JC, Kiselica D, Dunn M, Hendley JO. Latex agglutination testing directly from throat swabs for rapid detection of beta-hemolytic streptococci from Lancefield serogroup C.. J Clin Microbiol 1992 Mar;30(3):716-8.
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