Rapid detection of viral-specific antibodies by enzyme-linked immunosorbent assay (ELISA).
Abstract: The development of three separate rapid ELISAs for detecting antibodies in host serum to three different viruses is described. These include: 1. A direct antigen assay using enzyme labelled anti-canine Ig for detecting antibodies to canine parvovirus, 2. A competitive ELISA using a feline infectious peritonitis virus-specific monoclonal antibody labelled with enzyme, and 3. A competitive ELISA using an equine infectious anemia virus-specific monoclonal antibody and enzyme labelled antigen, p. 26. The utility and benefits of each of the three approaches is emphasized.
Publication Date: 1987-12-01 PubMed ID: 2829416PubMed Central: PMC7133761DOI: 10.1016/0165-2427(87)90161-9Google Scholar: Lookup
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- Journal Article
- Antibodies
- Antigen
- Clinical Study
- Diagnosis
- Diagnostic Technique
- Disease Diagnosis
- Disease Treatment
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Equine Diseases
- Equine Health
- Immunoglobulins
- Immunology
- In Vitro Research
- Infectious Disease
- Laboratory Methods
- Monoclonal Antibodies
- Serology
- Veterinary Medicine
- Veterinary Research
- Virology
- Virus
Summary
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This study describes the creation of three types of rapid enzyme-linked immunosorbent assays (ELISAs) to detect antibodies reacting to specific viruses in their host’s bloodstream.
Research Goals and Objectives
- The primary objective of this research was to develop three distinct rapid ELISAs. These assays aim to detect antibodies in the host’s serum that are specific to different viruses.
- The research also sought to emphasize the utility and advantages of these three types of ELISAs.
Types of ELISAs Developed
- The first is a direct antigen assay using an enzyme labelled anti-canine Ig. This ELISA was designed to detect antibodies that respond to the canine parvovirus.
- The second one is a competitive ELISA that uses an enzyme-labelled feline infectious peritonitis virus-specific monoclonal antibody. This ELISA’s purpose is to detect antibodies combating the feline infectious peritonitis virus.
- The third ELISA is also a competitive type but uses an enzyme labelled antigen, p. 26, and a monoclonal antibody specific to the equine infectious anemia virus to attract and detect antibodies in response to this virus in the host serum.
Benefits of the ELISAs
- Rapid ELISAs provide a quickly available result, which can be crucial in diagnosing viral infections promptly and starting treatment.
- These ELISAs can also be vital because they can help in observing the presence of a past or ongoing infection in a host.
- Additionally, because these specific ELISAs are designed to detect specific viruses, this increases their usability in managing and controlling the spread of specific viral diseases in animal populations.
- Their development can be directed towards other viruses, therefore enhancing their adaptability to manage and control various viral diseases.
Cite This Article
APA
Winston S, Fiscus S, Hesterberg L, Matsushita T, Mildbrand M, Porter J, Teramoto Y.
(1987).
Rapid detection of viral-specific antibodies by enzyme-linked immunosorbent assay (ELISA).
Vet Immunol Immunopathol, 17(1-4), 453-464.
https://doi.org/10.1016/0165-2427(87)90161-9 Publication
Researcher Affiliations
- Allelix Inc., Mississauga, Ontario.
MeSH Terms
- Animals
- Antibodies, Monoclonal
- Antibodies, Viral / analysis
- Cat Diseases / immunology
- Cats / immunology
- Coronaviridae / immunology
- Coronaviridae Infections / immunology
- Coronaviridae Infections / veterinary
- Dog Diseases / immunology
- Dogs / immunology
- Enzyme-Linked Immunosorbent Assay
- Equine Infectious Anemia / immunology
- Horses / immunology
- Infectious Anemia Virus, Equine / immunology
- Parvoviridae / immunology
- Parvoviridae Infections / immunology
- Parvoviridae Infections / veterinary
- Peritonitis / immunology
- Peritonitis / veterinary
References
This article includes 10 references
- Acre WM, Edwards BG, Fulker RH, Bandy DM. Further studies on canine parvovirus maternal immunity and successful vaccination in kennel situations.. Vet. Med. Small Anim. Clin. 1983:913–916. June.
- Fiscus SA, Mildbrand MM, Gordon JC, Teramoto YA, Winston S. Rapid enzyme-linked immunosorbent assay for detecting antibodies to canine parvovirus.. Am J Vet Res 1985 Apr;46(4):859-63.
- Fiscus SA, Teramoto YA, Mildbrand MM, Knisley CV, Winston SE, Pedersen NC. Competitive enzyme immunoassays for the rapid detection of antibodies to feline infectious peritonitis virus polypeptides.. J Clin Microbiol 1985 Sep;22(3):395-401.
- Horzinek MC, Lutz H, Pedersen NC. Antigenic relationships among homologous structural polypeptides of porcine, feline, and canine coronaviruses.. Infect Immun 1982 Sep;37(3):1148-55.
- Issel CJ, Coggins L. Equine infectious anemia: current knowledge.. J Am Vet Med Assoc 1979 Apr 1;174(7):727-33.
- Matusushita T, Porter JP, Smith BJ, Newman LE. New diagnostic tests for detection of equine infectious anemia. Amer. Assn. Vet. Lab. Diagnostics Annual proceedings 1984; pp. 27–34.
- Montelaro RC, Lohrey N, Parekh B, Blakeney EW, Issel CJ. Isolation and comparative biochemical properties of the major internal polypeptides of equine infectious anemia virus.. J Virol 1982 Jun;42(3):1029-38.
- Pedersen NC, Boyle JF, Floyd K, Fudge A, Barker J. An enteric coronavirus infection of cats and its relationship to feline infectious peritonitis.. Am J Vet Res 1981 Mar;42(3):368-77.
- Pollock RV, Carmichael LE. Maternally derived immunity to canine parvovirus infection: transfer, decline, and interference with vaccination.. J Am Vet Med Assoc 1982 Jan 1;180(1):37-42.
- Wallace BL, McMillen JK, Todd JD. Canine parvovirus serum neutralizing antibody assay: assessment of factors responsible for disparity of results between tests.. Cornell Vet 1983 Jan;73(1):52-7.
Citations
This article has been cited 2 times.- Langemeier JL, Cook SJ, Cook RF, Rushlow KE, Montelaro RC, Issel CJ. Detection of equine infectious anemia viral RNA in plasma samples from recently infected and long-term inapparent carrier animals by PCR. J Clin Microbiol 1996 Jun;34(6):1481-7.
- Rukhadze GG, Aliper TI, Sergeev VA. Isolation of peplomer glycoprotein E2 of transmissible gastroenteritis virus and application in enzyme-linked immunosorbent assay. J Clin Microbiol 1989 Aug;27(8):1754-8.
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