REAC technology as optimizer of stallion spermatozoa liquid storage.
Abstract: REAC technology (acronym for Radio Electric Asymmetric Conveyor) is a technology platform for neuro and bio modulation. It has already proven to optimize the ions fluxes at the molecular level and the molecular mechanisms driving cellular asymmetry and polarization. Methods: This study was designed to verify whether this technology could extend spermatozoa life-span during liquid storage, while preserving their functions, DNA integrity and oxidative status. At 0, 24, 48, and 72 h. of storage at 4 °C, a battery of analyses was performed to assess spermatozoa viability, motility parameters, acrosome status, and DNA integrity during REAC treatment. Spermatozoa oxidative status was assessed by determining lipid peroxidation, the activity of superoxide dismutase (SOD), and the total antioxidant capacity. Results: During liquid storage REAC treated spermatozoa, while not showing an increased viability nor motility compared to untreated ones, had a higher acrosome (p > 0.001) and DNA integrity (p > 0.01). Moreover, the analysis of the oxidative status indicated that the mean activity of the intracellular superoxide dismutase (SOD) was significantly higher in REAC treated spermatozoa compared to untreated controls (p 0.05). The REAC efficacy on spermatozoa oxidative status was also evidenced by the higher trolox equivalent antioxidant capacity (TEAC) found in both the cellular extract (p < 0.05) and the storage media of REAC treated spermatozoa compared to untreated controls (p < 0.0001). Conclusions: The present study demonstrated that REAC treatment during liquid storage preserves spermatozoa acrosome membrane and DNA integrity, likely due to the enhancement of sperm antioxidant defenses. These results open new perspective about the extending of spermatozoa functions in vitro and the clinical management of male infertility.
Publication Date: 2017-02-08 PubMed ID: 28179013PubMed Central: PMC5299698DOI: 10.1186/s12958-017-0229-6Google Scholar: Lookup
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- Journal Article
Summary
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The research article discusses a study examining how Radio Electric Asymmetric Conveyor (REAC) technology can prolong the lifespan and preserve the functionality of sperm during liquid storage. Their findings suggest that while it does not increase viability or mobility, it does safeguard acrosome membrane and genetic stability, potentially opening up new possibilities in male fertility management.
Research Methodology
- The research was carried out to determine whether REAC technology could extend the longevity of spermatozoa and preserve their functions while also maintaining their DNA structure and oxidative status during liquid storage.
- The authors performed a series of analyses at 0, 24, 48, and 72 hours of storage at 4°C to assess the viability, motility parameters, acrosome status, and DNA integrity of the sperm during the REAC treatment.
- The sperm’s oxidative status was evaluated by measuring lipid peroxidation, the activity of superoxide dismutase (SOD), and the overall antioxidant capacity.
Research Findings
- The results demonstrated that while the REAC treated sperm did not show any increase in viability or motility compared to untreated ones, it did show higher acrosome and DNA integrity.
- The researchers found that the average activity of superoxide dismutase (SOD), an enzyme that protects cells from oxidative damage, was significantly higher in the REAC treated sperm compared to untreated controls.
- The concentration of malondialdehyde (MDA), a by-product of lipid peroxidation which can show oxidative stress, at the end of the REAC treatment, was higher in untreated controls.
- The REAC treatment also showed higher trolox equivalent antioxidant capacity (TEAC) in both the cellular extract and the storage medium of the REAC-treated sperm, indicating its beneficial effect on oxidative status
Conclusion and Implications
- The study concluded that REAC treatment during liquid storage effectively preserved the integrity of spermatozoa’s acrosome membrane and DNA, possibly due to the enhancement of sperm’s antioxidant defences.
- The successful application of REAC technology to sperm preservation opens a new perspective on extending in-vitro sperm functions and can potentially influence the clinical management of male infertility.
Cite This Article
APA
Berlinguer F, Pasciu V, Succu S, Cossu I, Caggiu S, Addis D, Castagna A, Fontani V, Rinaldi S, Passino ES.
(2017).
REAC technology as optimizer of stallion spermatozoa liquid storage.
Reprod Biol Endocrinol, 15(1), 11.
https://doi.org/10.1186/s12958-017-0229-6 Publication
Researcher Affiliations
- Department of Veterinary Medicine, University of Sassari, Viale Vienna 43/B, 07100, Sassari, Italy.
- Department of Veterinary Medicine, University of Sassari, Viale Vienna 43/B, 07100, Sassari, Italy.
- Department of Veterinary Medicine, University of Sassari, Viale Vienna 43/B, 07100, Sassari, Italy.
- AGRIS, Department of Research for Equine Reproduction, Ozieri, Sassari, Italy.
- Department of Veterinary Medicine, University of Sassari, Viale Vienna 43/B, 07100, Sassari, Italy.
- Department of Veterinary Medicine, University of Sassari, Viale Vienna 43/B, 07100, Sassari, Italy.
- Departments of Regenerative Medicine, Rinaldi Fontani Institute, Viale Belfiore 43, 50144, Florence, Italy.
- Departments of Regenerative Medicine, Rinaldi Fontani Institute, Viale Belfiore 43, 50144, Florence, Italy.
- Research Department, Rinaldi Fontani Foundation, Viale Belfiore 43, 50144, Florence, Italy.
- Departments of Regenerative Medicine, Rinaldi Fontani Institute, Viale Belfiore 43, 50144, Florence, Italy. srinaldi@irf.it.
- Research Department, Rinaldi Fontani Foundation, Viale Belfiore 43, 50144, Florence, Italy. srinaldi@irf.it.
- Department of Veterinary Medicine, University of Sassari, Viale Vienna 43/B, 07100, Sassari, Italy.
MeSH Terms
- Acrosome / metabolism
- Animals
- Antioxidants / metabolism
- Cell Survival / genetics
- Comet Assay
- Cryopreservation / methods
- Cryopreservation / veterinary
- DNA / genetics
- DNA / metabolism
- Horses
- Lipid Peroxidation
- Male
- Malondialdehyde / metabolism
- Microscopy, Fluorescence
- Semen Analysis / methods
- Semen Analysis / veterinary
- Semen Preservation / methods
- Semen Preservation / veterinary
- Sperm Motility / genetics
- Spermatozoa / cytology
- Spermatozoa / metabolism
- Superoxide Dismutase / metabolism
References
This article includes 40 references
Citations
This article has been cited 8 times.- Contreras-Solís I, Pasciu V, Porcu C, Sotgiu FD, Todorova N, Baralla E, Mara L, Gallus M, Cabiddu A, Dattena M, Abecia JA, Berlinguer F. The Effect of By-Pass Linseed Oil Supplementation on the Maternal Antioxidant System during the Embryo-Maternal Recognition Period in Ewes.. Animals (Basel) 2023 Aug 9;13(16).
- Pasciu V, Nieddu M, Sotgiu FD, Baralla E, Berlinguer F. An Overview on Assay Methods to Quantify ROS and Enzymatic Antioxidants in Erythrocytes and Spermatozoa of Small Domestic Ruminants.. Animals (Basel) 2023 Jul 13;13(14).
- Pellegata G, Caracci S, Medaglini S. Radio Electric Asymmetric Conveyer Neurobiological Treatments in Non-Specific Neck Pain: A Retrospective Study.. J Pain Res 2020;13:2451-2459.
- Li CY, Hao HS, Zhao YH, Zhang PP, Wang HY, Pang YW, Du WH, Zhao SJ, Liu Y, Huang JM, Wang JJ, Ruan WM, Hao T, Reiter RJ, Zhu HB, Zhao XM. Melatonin Improves the Fertilization Capacity of Sex-Sorted Bull Sperm by Inhibiting Apoptosis and Increasing Fertilization Capacitation via MT1.. Int J Mol Sci 2019 Aug 12;20(16).
- Rinaldi A, Rinaldi C, Coelho Pereira JA, Lotti Margotti M, Bittencourt MN, Barcessat ARP, Fontani V, Rinaldi S. Radio electric asymmetric conveyer neuromodulation in depression, anxiety, and stress.. Neuropsychiatr Dis Treat 2019;15:469-480.
- Basoli V, Santaniello S, Rinaldi S, Fontani V, Pigliaru G, Wieser M, Strajeriu A, Castagna A, Redl H, Ventura C, Grillari R, Maioli M. Physical stimulation by REAC and BMP4/WNT-1 inhibitor synergistically enhance cardiogenic commitment in iPSCs.. PLoS One 2019;14(1):e0211188.
- Coelho Pereira JA, Rinaldi A, Fontani V, Rinaldi S. REAC neuromodulation treatments in subjects with severe socioeconomic and cultural hardship in the Brazilian state of Pará: a family observational pilot study.. Neuropsychiatr Dis Treat 2018;14:1047-1054.
- Sanna Passino E, Rocca S, Caggiu S, Columbano N, Castagna A, Fontani V, Rinaldi S. REAC regenerative treatment efficacy in experimental chondral lesions: a pilot study on ovine animal model.. Clin Interv Aging 2017;12:1471-1479.
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