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Home / Equine Research Bank / Vet Res Commun / Reaginic antibodies from horses with recurrent airway obstru...
Veterinary research communications2012; 36(4); 251-258; doi: 10.1007/s11259-012-9534-x

Reaginic antibodies from horses with recurrent airway obstruction produce mast cell stimulation.

Abstract: Reaginic antibodies (IgE and some IgG subclasses) and mast cells play important roles in the induction of type I immediate hypersensitivity reactions. These antibodies bind through their Fc fragment to high affinity receptors (FcεRI) present in the membrane of mast cells and basophils. The cross-linking of the receptor initiates a coordinated sequence of biochemical and morphological events that results in exocytosis of secretory granules containing pre-formed inflammatory mediators, secretion of newly formed lipid mediators, and secretion of cytokines. Previously, several studies have investigated the role of reaginic antibodies in the pathogenesis of Recurrent Airway Obstruction (RAO). However, whereas the immunological aspects of RAO have been extensively studied, the precise sequence of events involved in the pathogenesis remains not completely understood, and the role of IgE in this disease remains controversial. Therefore, in this study, several bioassays were conducted to determine whether reaginic antibodies from RAO-affected horses have the ability to activate mast cells. These bioassays involved measuring degranulation of rat peritoneal mast cells, activation of NF-κB and morphological changes in basophilic leukemia cells (RBL-2H3) following incubation with horse serum from RAO-affected horses that were sensitive and insensitive to Aspergillus fumigatus (A. fumigatus) or from unaffected horses. Our results show that reaginic antibodies from horses sensitive to A. fumigatus were able to degranulate rat peritoneal mast cells. In additon, there was an increase in the activity of the transcription factor NF-κB in RBL-2H3 cells, and morphological changes were observed in these cells once cross-linking was produced. These findings were not found in horses not sensitive to A. fumigatus and healthy horses. These bioassays demonstrate the ability of reaginic antibodies to stimulate mast cells and indicate that these antibodies could be involved in the immunological mechanisms leading to RAO.
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Publication Date: 2012-08-12 PubMed ID: 23011757DOI: 10.1007/s11259-012-9534-xGoogle Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The study investigates the role of specific antibodies, called reaginic antibodies, in triggering inflammation in horses with Recurrent Airway Obstruction (RAO), a respiratory disease. The results reveal that reaginic antibodies react with certain mast cells, potentially contributing to the disease’s progression.

Research Background

  • The study deals with the role of reaginic antibodies, primarily IgE and some IgG subclasses, in contributing to hypersensitivity reactions.
  • Mast cells and reaginic antibodies are known to play significant roles in type I immediate hypersensitivity reactions.
  • When these antibodies bind to receptors present on the membrane of mast cells and basophils, they initiate a series of biochemical and morphological events that lead to the release of certain substances that further inflammation.
  • Several studies have explored the role of these antibodies in horses suffering from RAO. However, the exact sequence of events in the pathogenesis of RAO remains unclear, and the role of IgE in this disease is also controversial.

Research Methodology

  • To investigate the potential role of these antibodies in RAO, researchers conducted various bioassays to determine if reaginic antibodies from RAO-affected horses can activate mast cells.
  • The trials measured the degranulation of rat peritoneal mast cells and evaluated the activation of the transcription factor NF-κB in basophilic leukemia cells (RBL-2H3) when incubated with serum from horses affected by RAO.
  • The serum used was from horses either sensitive or insensitive to Aspergillus fumigatus, a fungus known to cause respiratory issues, or from horses not affected by RAO.

Research Findings

  • The results showed that reaginic antibodies from horses sensitive to Aspergillus fumigatus could degranulate rat peritoneal mast cells.
  • The bioassays also revealed increased activity of the transcription factor NF-κB in the basophilic leukemia cells and noted morphological changes in these cells once cross-linking was produced.
  • These responses, however, were not noted in horses insensitive to Aspergillus fumigatus or in healthy horses.

Research Implications

  • The ability of reaginic antibodies to stimulate mast cells, as demonstrated by the bioassays, suggests that these antibodies could play a crucial role in the immunological mechanisms leading to RAO.
  • Understanding this could lead to new treatment strategies for managing RAO in horses.

Cite This Article

APA
Moran G, Folch H, Henriquez C, Ortloff A, Barria M. (2012). Reaginic antibodies from horses with recurrent airway obstruction produce mast cell stimulation. Vet Res Commun, 36(4), 251-258. https://doi.org/10.1007/s11259-012-9534-x

Publication

Veterinary research communications
ISSN: 1573-7446
NlmUniqueID: 8100520
Country: Switzerland
Language: English
Volume: 36
Issue: 4
Pages: 251-258

Researcher Affiliations

Moran, G
  • Department of Pharmacology, Faculty of Veterinary Science, Universidad Austral de Chile, 567, Valdivia, Chile. gmoran@uach.cl
Folch, H
    Henriquez, C
      Ortloff, A
        Barria, M

          MeSH Terms

          • Airway Obstruction / blood
          • Airway Obstruction / immunology
          • Airway Obstruction / veterinary
          • Animals
          • Antigens, Fungal / blood
          • Aspergillus fumigatus / immunology
          • Biological Assay / methods
          • Biological Assay / veterinary
          • Horse Diseases / immunology
          • Horses
          • Immunoglobulin G / immunology
          • Leukemia, Basophilic, Acute / immunology
          • Mast Cells / immunology
          • NF-kappa B / immunology
          • Rats
          • Reagins / blood

          References

          This article includes 55 references
          1. Suter M, Fey H. Isolation and characterization of equine IgE.. Zentralbl Veterinarmed B 1981;28(5):414-20.
            pubmed: 6798787doi: 10.1111/j.1439-0450.1981.tb01930.xgoogle scholar: lookup
          2. Okayama Y, Hagaman DD, Metcalfe DD. A comparison of mediators released or generated by IFN-gamma-treated human mast cells following aggregation of Fc gamma RI or Fc epsilon RI.. J Immunol 2001 Apr 1;166(7):4705-12.
            pubmed: 11254731doi: 10.4049/jimmunol.166.7.4705google scholar: lookup
          3. Curik I, Fraser D, Eder C, Achmann R, Swinburne J, Crameri R, Brem G, Sölkner J, Marti E. Association between the MHC gene region and variation of serum IgE levels against specific mould allergens in the horse.. Genet Sel Evol 2003;35 Suppl 1(Suppl 1):S177-90.
            pubmed: 12927090doi: 10.1186/1297-9686-35-S1-S177google scholar: lookup
          4. Baldwin AS Jr. The NF-kappa B and I kappa B proteins: new discoveries and insights.. Annu Rev Immunol 1996;14:649-83.
            pubmed: 8717528doi: 10.1146/annurev.immunol.14.1.649google scholar: lookup
          5. Dowdall SM, Proudman CJ, Klei TR, Mair T, Matthews JB. Characterisation of IgG(T) serum antibody responses to two larval antigen complexes in horses naturally- or experimentally-infected with cyathostomins.. Int J Parasitol 2004 Jan;34(1):101-8.
            pubmed: 14711595doi: 10.1016/j.ijpara.2003.09.008google scholar: lookup
          6. Maeyama K, Hohman RJ, Metzger H, Beaven MA. Quantitative relationships between aggregation of IgE receptors, generation of intracellular signals, and histamine secretion in rat basophilic leukemia (2H3) cells. Enhanced responses with heavy water.. J Biol Chem 1986 Feb 25;261(6):2583-92.
            pubmed: 2419319
          7. Halliwell RE, McGorum BC, Irving P, Dixon PM. Local and systemic antibody production in horses affected with chronic obstructive pulmonary disease.. Vet Immunol Immunopathol 1993 Oct;38(3-4):201-15.
            pubmed: 8291200doi: 10.1016/0165-2427(93)90081-egoogle scholar: lookup
          8. Barnes PJ, Adcock IM. Transcription factors and asthma.. Eur Respir J 1998 Jul;12(1):221-34.
            pubmed: 9701442doi: 10.1183/09031936.98.12010221google scholar: lookup
          9. Sandersen C, Bureau F, Turlej R, Fiévez L, Dogné S, Kirschvink N, Lekeux P. p65 Homodimer activity in distal airway cells determines lung dysfunction in equine heaves.. Vet Immunol Immunopathol 2001 Aug 10;80(3-4):315-26.
            pubmed: 11457483doi: 10.1016/s0165-2427(01)00329-4google scholar: lookup
          10. Fewtrell C, Metzger H. Larger oligomers of IgE are more effective than dimers in stimulating rat basophilic leukemia cells.. J Immunol 1980 Aug;125(2):701-10.
            pubmed: 7391576
          11. Tkaczyk C, Gilfillan AM. Fc(epsilon)Ri-dependent signaling pathways in human mast cells.. Clin Immunol 2001 May;99(2):198-210.
            pubmed: 11318592doi: 10.1006/clim.2001.4992google scholar: lookup
          12. Latour S, Bonnerot C, Fridman WH, Daëron M. Induction of tumor necrosis factor-alpha production by mast cells via Fc gamma R. Role of the Fc gamma RIII gamma subunit.. J Immunol 1992 Sep 15;149(6):2155-62.
            pubmed: 1387672
          13. Galli SJ, Nakae S, Tsai M. Mast cells in the development of adaptive immune responses.. Nat Immunol 2005 Feb;6(2):135-42.
            pubmed: 15662442doi: 10.1038/ni1158google scholar: lookup
          14. Eder C, Curik I, Brem G, Crameri R, Bodo I, Habe F, Lazary S, Sölkner J, Marti E. Influence of environmental and genetic factors on allergen-specific immunoglobulin-E levels in sera from Lipizzan horses.. Equine Vet J 2001 Nov;33(7):714-20.
            pubmed: 11770995doi: 10.2746/042516401776249264google scholar: lookup
          15. Barnes PJ, Chung KF, Page CP. Inflammatory mediators of asthma: an update.. Pharmacol Rev 1998 Dec;50(4):515-96.
            pubmed: 9860804
          16. Barnes PJ, Karin M. Nuclear factor-kappaB: a pivotal transcription factor in chronic inflammatory diseases.. N Engl J Med 1997 Apr 10;336(15):1066-71.
            pubmed: 9091804doi: 10.1056/NEJM199704103361506google scholar: lookup
          17. van der Haegen A, Künzle F, Gerber V, Welle M, Robinson NE, Marti E. Mast cells and IgE-bearing cells in lungs of RAO-affected horses.. Vet Immunol Immunopathol 2005 Dec 15;108(3-4):325-34.
            pubmed: 16040130doi: 10.1016/j.vetimm.2005.06.005google scholar: lookup
          18. Schmallenbach KH, Rahman I, Sasse HH, Dixon PM, Halliwell RE, McGorum BC, Crameri R, Miller HR. Studies on pulmonary and systemic Aspergillus fumigatus-specific IgE and IgG antibodies in horses affected with chronic obstructive pulmonary disease (COPD).. Vet Immunol Immunopathol 1998 Dec 11;66(3-4):245-56.
            pubmed: 9880102doi: 10.1016/s0165-2427(98)00202-5google scholar: lookup
          19. Ishizaka K, Ishizaka T. Physicochemical properties of reaginic antibody. 1. Association of reaginic activity with an immunoglobulin other than gammaA- or gammaG-globulin.. J Allergy 1966 Mar;37(3):169-85.
            pubmed: 4160257doi: 10.1016/0021-8707(66)90091-8google scholar: lookup
          20. Wagner B. IgE in horses: occurrence in health and disease.. Vet Immunol Immunopathol 2009 Nov 15;132(1):21-30.
            pubmed: 19819562doi: 10.1016/j.vetimm.2009.09.011google scholar: lookup
          21. Morán G, Burgos R, Araya O, Folch H. In vitro bioassay to detect reaginic antibodies from the serum of horses affected with recurrent airway obstruction.. Vet Res Commun 2010 Jan;34(1):91-9.
            pubmed: 20012188doi: 10.1007/s11259-009-9334-0google scholar: lookup
          22. Robertson D, Holowka D, Baird B. Cross-linking of immunoglobulin E-receptor complexes induces their interaction with the cytoskeleton of rat basophilic leukemia cells.. J Immunol 1986 Jun 15;136(12):4565-72.
            pubmed: 2423596
          23. Bureau F, Delhalle S, Bonizzi G, Fiévez L, Dogné S, Kirschvink N, Vanderplasschen A, Merville MP, Bours V, Lekeux P. Mechanisms of persistent NF-kappa B activity in the bronchi of an animal model of asthma.. J Immunol 2000 Nov 15;165(10):5822-30.
            pubmed: 11067942doi: 10.4049/jimmunol.165.10.5822google scholar: lookup
          24. Finotto S, De Sanctis GT, Lehr HA, Herz U, Buerke M, Schipp M, Bartsch B, Atreya R, Schmitt E, Galle PR, Renz H, Neurath MF. Treatment of allergic airway inflammation and hyperresponsiveness by antisense-induced local blockade of GATA-3 expression.. J Exp Med 2001 Jun 4;193(11):1247-60.
            pubmed: 11390432doi: 10.1084/jem.193.11.1247google scholar: lookup
          25. Robinson NE. International Workshop on Equine Chronic Airway Disease. Michigan State University 16-18 June 2000.. Equine Vet J 2001 Jan;33(1):5-19.
            pubmed: 11191611doi: 10.2746/042516401776767412google scholar: lookup
          26. Yamashita M, Onodera A, Nakayama T. Immune mechanisms of allergic airway disease: regulation by transcription factors.. Crit Rev Immunol 2007;27(6):539-46.
            pubmed: 18197799doi: 10.1615/critrevimmunol.v27.i6.40google scholar: lookup
          27. Robinson NE, Olszewski MA, Boehler D, Berney C, Hakala J, Matson C, Derksen FJ. Relationship between clinical signs and lung function in horses with recurrent airway obstruction (heaves) during a bronchodilator trial.. Equine Vet J 2000 Sep;32(5):393-400.
            pubmed: 11037260doi: 10.2746/042516400777591147google scholar: lookup
          28. Pelletier C, Varin-Blank N, Rivera J, Iannascoli B, Marchand F, David B, Weyer A, Blank U. Fc epsilonRI-mediated induction of TNF-alpha gene expression in the RBL-2H3 mast cell line: regulation by a novel NF-kappaB-like nuclear binding complex.. J Immunol 1998 Nov 1;161(9):4768-76.
            pubmed: 9794408
          29. Hellberg W, Wilson AD, Mellor P, Doherr MG, Torsteinsdottir S, Zurbriggen A, Jungi T, Marti E. Equine insect bite hypersensitivity: immunoblot analysis of IgE and IgG subclass responses to Culicoides nubeculosus salivary gland extract.. Vet Immunol Immunopathol 2006 Sep 15;113(1-2):99-112.
            pubmed: 16797724doi: 10.1016/j.vetimm.2006.04.009google scholar: lookup
          30. Katz HR, Lobell RB. Expression and function of Fc gamma R in mouse mast cells.. Int Arch Allergy Immunol 1995 May-Jun;107(1-3):76-8.
            pubmed: 7613221doi: 10.1159/000236936google scholar: lookup
          31. Bureau F, Bonizzi G, Kirschvink N, Delhalle S, Desmecht D, Merville MP, Bours V, Lekeux P. Correlation between nuclear factor-kappaB activity in bronchial brushing samples and lung dysfunction in an animal model of asthma.. Am J Respir Crit Care Med 2000 Apr;161(4 Pt 1):1314-21.
            pubmed: 10764329doi: 10.1164/ajrccm.161.4.9907010google scholar: lookup
          32. Chen ZJ, Parent L, Maniatis T. Site-specific phosphorylation of IkappaBalpha by a novel ubiquitination-dependent protein kinase activity.. Cell 1996 Mar 22;84(6):853-62.
            pubmed: 8601309doi: 10.1016/s0092-8674(00)81064-8google scholar: lookup
          33. Kulczycki A Jr, Isersky C, Metzger H. The interaction of IgE with rat basophilic leukemia cells. I. Evidence for specific binding of IgE.. J Exp Med 1974 Mar 1;139(3):600-16.
            pubmed: 4812630doi: 10.1084/jem.139.3.600google scholar: lookup
          34. Ali H, Maeyama K, Sagi-Eisenberg R, Beaven MA. Antigen and thapsigargin promote influx of Ca2+ in rat basophilic RBL-2H3 cells by ostensibly similar mechanisms that allow filling of inositol 1,4,5-trisphosphate-sensitive and mitochondrial Ca2+ stores.. Biochem J 1994 Dec 1;304 ( Pt 2)(Pt 2):431-40.
            pubmed: 7998977doi: 10.1042/bj3040431google scholar: lookup
          35. Okayama Y, Tkaczyk C, Metcalfe DD, Gilfillan AM. Comparison of Fc epsilon RI- and Fc gamma RI-mediated degranulation and TNF-alpha synthesis in human mast cells: selective utilization of phosphatidylinositol-3-kinase for Fc gamma RI-induced degranulation.. Eur J Immunol 2003 May;33(5):1450-9.
            pubmed: 12731072doi: 10.1002/eji.200323563google scholar: lookup
          36. Robinson NE, Derksen FJ, Olszewski MA, Buechner-Maxwell VA. The pathogenesis of chronic obstructive pulmonary disease of horses.. Br Vet J 1996 May;152(3):283-306.
            pubmed: 8762605doi: 10.1016/s0007-1935(96)80101-1google scholar: lookup
          37. Mezzano SA, Barría M, Droguett MA, Burgos ME, Ardiles LG, Flores C, Egido J. Tubular NF-kappaB and AP-1 activation in human proteinuric renal disease.. Kidney Int 2001 Oct;60(4):1366-77.
            pubmed: 11576350doi: 10.1046/j.1523-1755.2001.00941.xgoogle scholar: lookup
          38. Derksen FJ, Robinson NE, Armstrong PJ, Stick JA, Slocombe RF. Airway reactivity in ponies with recurrent airway obstruction (heaves).. J Appl Physiol (1985) 1985 Feb;58(2):598-604.
            pubmed: 3980362doi: 10.1152/jappl.1985.58.2.598google scholar: lookup
          39. Ikawati Z, Wahyuono S, Maeyama K. Screening of several Indonesian medicinal plants for their inhibitory effect on histamine release from RBL-2H3 cells.. J Ethnopharmacol 2001 May;75(2-3):249-56.
            pubmed: 11297859doi: 10.1016/s0378-8741(01)00201-xgoogle scholar: lookup
          40. Kobayashi H, Okayama Y, Ishizuka T, Pawankar R, Ra C, Mori M. Production of IL-13 by human lung mast cells in response to Fcepsilon receptor cross-linkage.. Clin Exp Allergy 1998 Oct;28(10):1219-27.
            pubmed: 9824388doi: 10.1046/j.1365-2222.1998.00377.xgoogle scholar: lookup
          41. Künzle F, Gerber V, Van Der Haegen A, Wampfler B, Straub R, Marti E. IgE-bearing cells in bronchoalveolar lavage fluid and allergen-specific IgE levels in sera from RAO-affected horses.. J Vet Med A Physiol Pathol Clin Med 2007 Feb;54(1):40-7.
            pubmed: 17359454doi: 10.1111/j.1439-0442.2007.00870.xgoogle scholar: lookup
          42. Ching TT, Hsu AL, Johnson AJ, Chen CS. Phosphoinositide 3-kinase facilitates antigen-stimulated Ca(2+) influx in RBL-2H3 mast cells via a phosphatidylinositol 3,4,5-trisphosphate-sensitive Ca(2+) entry mechanism.. J Biol Chem 2001 May 4;276(18):14814-20.
            pubmed: 11278575doi: 10.1074/jbc.M009851200google scholar: lookup
          43. Prouvost-Danon A, Binaghi RA, Abadie A. Effect of heating at 56 degrees C on mouse IgE antibodies.. Immunochemistry 1977 Feb;14(2):81-4.
            pubmed: 15944doi: 10.1016/0019-2791(77)90284-1google scholar: lookup
          44. Astorquiza MI, Fernández S. Anaphylaxis inhibitory factor in IgE-dependent mast cell stimulation.. J Investig Allergol Clin Immunol 1999 Sep-Oct;9(5):330-4.
            pubmed: 10582202
          45. Marti E, Gerber V, Wilson AD, Lavoie JP, Horohov D, Crameri R, Lunn DP, Antczak D, Björnsdóttir S, Björnsdóttir TS, Cunningham F, Dérer M, Frey R, Hamza E, Horin P, Heimann M, Kolm-Stark G, Olafsdóttir G, Ramery E, Russell C, Schaffartzik A, Svansson V, Torsteinsdóttir S, Wagner B. Report of the 3rd Havemeyer workshop on allergic diseases of the Horse, Hólar, Iceland, June 2007.. Vet Immunol Immunopathol 2008 Dec 15;126(3-4):351-61.
            pubmed: 18775570doi: 10.1016/j.vetimm.2008.07.008google scholar: lookup
          46. Eder C, Crameri R, Mayer C, Eicher R, Straub R, Gerber H, Lazary S, Marti E. Allergen-specific IgE levels against crude mould and storage mite extracts and recombinant mould allergens in sera from horses affected with chronic bronchitis.. Vet Immunol Immunopathol 2000 Mar 15;73(3-4):241-53.
            pubmed: 10713338doi: 10.1016/s0165-2427(00)00154-9google scholar: lookup
          47. Nguyen C, Teo JL, Matsuda A, Eguchi M, Chi EY, Henderson WR Jr, Kahn M. Chemogenomic identification of Ref-1/AP-1 as a therapeutic target for asthma.. Proc Natl Acad Sci U S A 2003 Feb 4;100(3):1169-73.
            pubmed: 12552119doi: 10.1073/pnas.0437889100google scholar: lookup
          48. Kim TD, Eddlestone GT, Mahmoud SF, Kuchtey J, Fewtrell C. Correlating Ca2+ responses and secretion in individual RBL-2H3 mucosal mast cells.. J Biol Chem 1997 Dec 12;272(50):31225-9.
            pubmed: 9395446doi: 10.1074/jbc.272.50.31225google scholar: lookup
          49. Jackson KA, Stott JL, Horohov DW, Watson JL. IL-4 induced CD23 (FcepsilonRII) up-regulation in equine peripheral blood mononuclear cells and pulmonary alveolar macrophages.. Vet Immunol Immunopathol 2004 Oct;101(3-4):243-50.
            pubmed: 15350754doi: 10.1016/j.vetimm.2004.05.001google scholar: lookup
          50. Hill DE, Forbes L, Kramer M, Gajadhar A, Gamble HR. Larval viability and serological response in horses with long-term Trichinella spiralis infection.. Vet Parasitol 2007 May 15;146(1-2):107-16.
            pubmed: 17386976doi: 10.1016/j.vetpar.2007.02.011google scholar: lookup
          51. Couëtil LL, Art T, de Moffarts B, Becker M, Mélotte D, Jaspar F, Bureau F, Lekeux P. DNA binding activity of transcription factors in bronchial cells of horses with recurrent airway obstruction.. Vet Immunol Immunopathol 2006 Sep 15;113(1-2):11-20.
            pubmed: 16753225doi: 10.1016/j.vetimm.2006.03.020google scholar: lookup
          52. Tahon L, Baselgia S, Gerber V, Doherr MG, Straub R, Robinson NE, Marti E. In vitro allergy tests compared to intradermal testing in horses with recurrent airway obstruction.. Vet Immunol Immunopathol 2009 Jan 15;127(1-2):85-93.
            pubmed: 19027178doi: 10.1016/j.vetimm.2008.09.021google scholar: lookup
          53. Gerber V, Lindberg A, Berney C, Robinson NE. Airway mucus in recurrent airway obstruction--short-term response to environmental challenge.. J Vet Intern Med 2004 Jan-Feb;18(1):92-7.
            pubmed: 14765737doi: 10.1892/0891-6640(2004)18<92:amirao>2.0.co;2google scholar: lookup
          54. Astorquiza MI, Helle B, Vergara RE. [Effect of vitamin A on the in vitro degranulation of mouse mast cells].. Allergol Immunopathol (Madr) 1980 Mar-Apr;8(2):87-90.
            pubmed: 6450523
          55. Morán G, Folch H, Araya O, Burgos R, Barria M. Detection of reaginic antibodies against Faenia rectivirgula from the serum of horses affected with Recurrent Airway Obstruction by an in vitro bioassay.. Vet Res Commun 2010 Dec;34(8):719-26.
            pubmed: 20814741doi: 10.1007/s11259-010-9440-zgoogle scholar: lookup

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