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Veterinary immunology and immunopathology2013; 156(3-4); 190-199; doi: 10.1016/j.vetimm.2013.09.020

Real time RT-PCR analysis of inflammatory mediator expression in recurrent airway obstruction-affected horses.

Abstract: The goal of the present study was to investigate mRNA expression levels of several cytokines and inflammatory mediators in broncho-alveolar lavage (BAL) fluid and respiratory epithelium in recurrent airway obstruction (RAO)-affected horses. RAO, also called heaves, is a common, performance-limiting, equine respiratory disease with clinical signs and pathophysiological similarities to human asthma, and characterized by bronchospasm, neutrophilic infiltration and increased mucus in the airways. Six RAO-affected horses were examined twice within 15 days and seven clinically healthy horses were examined for comparison. Quantitative real-time RT-PCR was used to assess mRNA expression of the inflammatory mediators IL-1β, IL-6, IL-8, IL-13, IL-17, TNFα, INFγ, TGFβ1, NFκ-β and TRL4 in bronchial biopsies and in BAL fluid. Gene expression levels were then compared with clinical signs, endoscopic examination, complete blood cell count, cytology of BAL fluid, histological examination of bronchial tissue and bacteriological and mycological examinations. Expression of IL1β, IL8, TLR4, TNFα, TGFβ1 and NFkβ transcripts was significantly up-regulated in RAO-affected compared to healthy horses. A similar trend, albeit not significant, was showed for IL17 and INFγ. A highly significant correlation was observed among IL-1β, IL8, TGFβ1, NFkβ, TRL4, and INFγ expression patterns as well as between expression levels of these genes and clinical parameters. In the present study, the comparison between clinically healthy and RAO-affected horses gave new insights on the cytokine expression in equine health and disease status. The identification of cytokines implicated in the pathogenesis of RAO may contribute to the diagnosis and treatment of this disease.
© 2013 Elsevier B.V. All rights reserved.
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Publication Date: 2013-10-08 PubMed ID: 24176614DOI: 10.1016/j.vetimm.2013.09.020Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research aims to probe the expression levels of various cytokines and inflammatory mediators in horse’s broncho-alveolar lavage (BAL) fluid and respiratory epithelium suffering from recurrent airway obstruction (RAO), a common equine respiratory disease akin to human asthma. A detailed study of the expressed genes, in comparison to healthy horses, provides insights into the potential diagnostic cues and treatment methods for RAO.

Methodology and Analysis

  • The study considered six horses affected with RAO, each examined twice within a 15-day window.
  • Seven healthy horses were engaged for comparative analysis.
  • Real-time RT-PCR, an effective molecular technique, was employed to quantify the mRNA expression of targeted inflammatory mediators like IL-1β, IL-6, IL-8, IL-13, IL-17, TNFα, INFγ, TGFβ1, NFκ-β and TRL4 from the bronchial biopsies and the BAL fluid.
  • In-depth comparison was carried out based on a range of factors such as clinical symptoms, endoscopic examination, complete blood cell count, BAL fluid cytology, bronze tissue histological examination, and tests for bacterial and fungal presence.

Results and Conclusion

  • This research revealed significant upregulation in the transcripts of IL1β, IL8, TLR4, TNFα, TGFβ1, and NFkβ in RAO-affected horses compared to healthy ones.
  • A similar upward-trending pattern in expression, albeit not statistically significant, was also identified for IL17 and INFγ.
  • The study observed a highly notable correlation between the expression patterns of inflammatory mediators (IL-1β, IL8, TGFβ1, NFkβ, TRL4, and INFγ) and clinical parameters of the disease.
  • The research findings on cytokine expression level variances between healthy and RAO-afflicted horses contribute to the understanding of cytokine patterns in equine health and pathological conditions.
  • Recognizing the cytokines involved in RAO’s pathogenesis could potentially assist in diagnosing and treating this respiratory disease in horses.

Cite This Article

APA
Padoan E, Ferraresso S, Pegolo S, Castagnaro M, Barnini C, Bargelloni L. (2013). Real time RT-PCR analysis of inflammatory mediator expression in recurrent airway obstruction-affected horses. Vet Immunol Immunopathol, 156(3-4), 190-199. https://doi.org/10.1016/j.vetimm.2013.09.020

Publication

Veterinary immunology and immunopathology
ISSN: 1873-2534
NlmUniqueID: 8002006
Country: Netherlands
Language: English
Volume: 156
Issue: 3-4
Pages: 190-199
PII: S0165-2427(13)00268-7

Researcher Affiliations

Padoan, E
  • Equine Patavium Hospital, via G.Trieste n 21, Limena, PD, Italy; Department of Comparative Biomedicine and Food Science (BCA), University of Padua, Viale dell'Universita' 16, 35020 Legnaro, PD, Italy. Electronic address: elisa.padoan.vet@gmail.com.
Ferraresso, S
    Pegolo, S
      Castagnaro, M
        Barnini, C
          Bargelloni, L

            MeSH Terms

            • Airway Obstruction / immunology
            • Airway Obstruction / veterinary
            • Animals
            • Biopsy
            • Bronchoalveolar Lavage Fluid / cytology
            • Bronchoalveolar Lavage Fluid / immunology
            • Horse Diseases / immunology
            • Horses
            • Inflammation Mediators / analysis
            • Real-Time Polymerase Chain Reaction / methods
            • Recurrence
            • Reverse Transcriptase Polymerase Chain Reaction / methods

            Citations

            This article has been cited 19 times.
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