Recent vesicular stomatitis virus infection detected by immunoglobulin M antibody capture enzyme-linked immunosorbent assay.
Abstract: We developed an enzyme-linked immunosorbent assay (ELISA) that was capable of detecting immunoglobulin M (IgM) antibody to vesicular stomatitis virus (VSV) in the sera of experimentally and naturally infected cattle and horses. The detection of IgM in the sera of these animals permitted an estimate of the recency of infection by VSV serotype New Jersey. A VSV serotype New Jersey epizootic strain isolated from a horse and passed once in an Aedes albopictus cell line was used to infect a horse and a calf. Sera from these animals were used to standardize the ELISA. This assay was used to test sera from cattle and horses involved in the 1982 VSV epizootic. Comparative antibody titrations were performed by three systems: the serum-dilution plaque-reduction neutralization, complement fixation, and indirect immunofluorescent tests. The antibody titers by neutralization and the ELISA were comparable for the period that IgM was present; when IgM ELISA titers diminished, the neutralization titers remained high. The complement fixation and indirect immunofluorescent antibody titers followed closely the IgM pattern determined by the ELISA. The capture IgM ELISA is applicable for the rapid detection of IgM antibody to VSV in cattle and horses and is a useful assay of recent infection.
Publication Date: 1985-10-01 PubMed ID: 3001132PubMed Central: PMC268471DOI: 10.1128/jcm.22.4.582-586.1985Google Scholar: Lookup
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- Comparative Study
- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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The research involves the development of an Enzyme-Linked Immunosorbent Assay (ELISA) test to detect the presence of Immunoglobulin M (IgM) antibodies specific to Vesicular Stomatitis Virus (VSV) in cattle and horses, offering an estimation of recent infections.
Development of the ELISA Test
- The researchers developed an ELISA test that detects the presence of Immunoglobulin M (IgM) antibodies against Vesicular Stomatitis Virus (VSV) in the blood serum of both experimentally and naturally infected horses and cattle.
- The presence of these specific IgM antibodies enabled the research team to estimate the recency of VSV infection within these animals, specifically for the VSV serotype New Jersey.
- The isolation of the VSV serotype New Jersey was conducted from an infected horse and then cultured once in an Aedes albopictus cell line. This strain served in the infection of another horse and calf to further standardize the ELISA.
The Use of ELISA Test on VSV Outbreak
- The newly developed assay was applied on serum samples collected from horses and cattle involved in a Vesicular Stomatitis Virus outbreak, which happened in 1982.
Comparative Antibody Titration
- The researchers performed comparative antibody titration using three different systems: The serum-dilution plaque-reduction neutralization, complement fixation, and indirect immunofluorescent tests.
- The antibody titers obtained from neutralization and the new ELISA test showed comparable results, particularly during the period when the IgM antibodies were present.
- As the presence of the IgM antibodies decreased, neutralization titers remained high, while the complement fixation and indirect immunofluorescent antibody titers closely followed the declining IgM pattern determined through the ELISA test.
Significance of the Research
- The outcome of this research points out that the developed IgM capture ELISA test is useful for the swift detection of IgM antibodies against VSV in horses and cattle, serving as a crucial tool to identify recent infections.
Cite This Article
APA
Vernon SD, Webb PA.
(1985).
Recent vesicular stomatitis virus infection detected by immunoglobulin M antibody capture enzyme-linked immunosorbent assay.
J Clin Microbiol, 22(4), 582-586.
https://doi.org/10.1128/jcm.22.4.582-586.1985 Publication
Researcher Affiliations
MeSH Terms
- Animals
- Antibodies, Viral / analysis
- Cattle
- Cattle Diseases / diagnosis
- Cattle Diseases / immunology
- Complement Fixation Tests
- Cross Reactions
- Enzyme-Linked Immunosorbent Assay
- Fluorescent Antibody Technique
- Horse Diseases / diagnosis
- Horse Diseases / immunology
- Horses
- Immunoglobulin M / analysis
- Neutralization Tests
- Stomatitis / diagnosis
- Stomatitis / immunology
- Stomatitis / veterinary
- Vesiculovirus / immunology
- Virus Diseases / diagnosis
- Virus Diseases / immunology
- Virus Diseases / veterinary
References
This article includes 12 references
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