Recombinant envelope protein (rgp90) ELISA for equine infectious anemia virus provides comparable results to the agar gel immunodiffusion.
Abstract: Equine infectious anemia (EIA) is an important viral infection affecting horses worldwide. The course of infection is accompanied generally by three characteristic stages: acute, chronic and inapparent. There is no effective EIA vaccine or treatment, and the control of the disease is based currently on identification of EIAV inapparent carriers by laboratory tests. Recombinant envelope protein (rgp90) was expressed in Escherichia coli and evaluated via enzyme-linked immunosorbent assay (ELISA). There was an excellent agreement (95.42%) between the ELISA results using rgp90 and agar gel immunodiffusion test results. AGID is considered the "gold-standard" serologic test for equine infectious anemia (EIA). After 1160 serum samples were tested, the relative sensitivity and specificity of the ELISA were 96.1% and 96.4%, respectively. Moreover, analysis diagnostic accuracy of the ELISA was performed. The ELISA proved robust. Furthermore, good reproducibility was observed for the negative controls and, positive controls for all plates tested.
Copyright © 2011 Elsevier B.V. All rights reserved.
Publication Date: 2011-12-30 PubMed ID: 22227617DOI: 10.1016/j.jviromet.2011.12.012Google Scholar: Lookup
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- Comparative Study
- Evaluation Study
- Journal Article
- Research Support
- Non-U.S. Gov't
- Comparative Study
- Diagnosis
- Diagnostic Technique
- Disease control
- Disease Diagnosis
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Epidemiology
- Equine Diseases
- Equine Health
- Equine Infectious Anemia
- Escherichia coli
- Horses
- Immunology
- Infection
- Infectious Disease
- Laboratory Methods
- Recombinant Proteins
- Serology
- Veterinary Medicine
- Veterinary Research
- Virus
Summary
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The research article discusses an experiment conducted to evaluate the effectiveness of a specific protein (rgp90) test in detecting Equine Infectious Anemia (EIA), in comparison to the currently favored standard test.
Research Background
- The article details research performed on Equine Infectious Anemia (EIA), a disease impacting horses globally. Known for its three stages – acute, chronic and inapparent – its detection is crucial to control as there’s no viable vaccine or treatment for it.
- Currently, the control of EIA is focused on identifying its occurrence in horses, particularly in the disease’s inapparent stage. This is generally accomplished using laboratory tests.
Research Process and Methodology
- The researchers focused on a protein named Recombinant envelope protein (rgp90) and sought out to compare its diagnostic effectiveness to Agar Gel Immunodiffusion (AGID) testing, the conventional standard test for EIA.
- This rgp90 protein was expressed in a common bacterial species, Escherichia coli, and the evaluation was performed using an Enzyme-Linked Immunosorbent Assay (ELISA).
- Through this comparison, the researchers wished to gauge whether it met the standards set by the customary ‘gold standard’ test.
Research Findings
- An outstanding agreement of 95.42% was found between the ELISA result using rgp90 and the AGID test.
- 1160 serum samples were stress-tested. The ELISA’s relative sensitivity and specificity were found to be 96.1% and 96.4% respectively.
- Moreover, the researchers conducted a detailed evaluation of the diagnostic accuracy of rgp90’s ELISA. It was judged to be robust and showed good reproducibility for both the negative and positive controls for all plates tested.
Conclusion
- This research article suggests that the ELISA using the rgp90 protein can serve as a potentially viable testing method for EIA, matching up to the ‘gold standard’ in detection and diagnostic accuracy.
Cite This Article
APA
Reis JK, Diniz RS, Haddad JP, Ferraz IB, Carvalho AF, Kroon EG, Ferreira PC, Leite RC.
(2011).
Recombinant envelope protein (rgp90) ELISA for equine infectious anemia virus provides comparable results to the agar gel immunodiffusion.
J Virol Methods, 180(1-2), 62-67.
https://doi.org/10.1016/j.jviromet.2011.12.012 Publication
Researcher Affiliations
- Departamento de Medicina Veterinária Preventiva, Escola de Veterinária, Brasil. jenner@ufmg.br
MeSH Terms
- Agar
- Animals
- Antibodies, Viral / blood
- Antigens, Viral / genetics
- Antigens, Viral / immunology
- Antigens, Viral / metabolism
- Enzyme-Linked Immunosorbent Assay
- Equine Infectious Anemia / diagnosis
- Gene Products, env / genetics
- Gene Products, env / immunology
- Gene Products, env / metabolism
- Horses
- Immunodiffusion
- Infectious Anemia Virus, Equine / immunology
- Recombinant Proteins / genetics
- Recombinant Proteins / immunology
- Recombinant Proteins / metabolism
- Reproducibility of Results
- Sensitivity and Specificity
Citations
This article has been cited 7 times.- Hu Z, Guo K, Du C, Sun J, Naletoski I, Chu X, Lin Y, Wang X, Barrandeguy M, Samuel M, Wang W, Lau PI, Wernery U, Raghavan R, Wang X. Development and evaluation of a blocking ELISA for serological diagnosis of equine infectious anemia. Appl Microbiol Biotechnol 2023 May;107(10):3305-3317.
- Malossi CD, Fioratti EG, Cardoso JF, Magro AJ, Kroon EG, Aguiar DM, Borges AMCM, Nogueira MF, Ullmann LS, Araujo JP Jr. High Genomic Variability in Equine Infectious Anemia Virus Obtained from Naturally Infected Horses in Pantanal, Brazil: An Endemic Region Case. Viruses 2020 Feb 12;12(2).
- Cruz F, Fores P, Ireland J, Moreno MA, Newton R. Freedom from equine infectious anaemia virus infection in Spanish Purebred horses. Vet Rec Open 2015;2(1):e000074.
- Singha H, Goyal SK, Malik P, Khurana SK, Singh RK. Development, evaluation, and laboratory validation of immunoassays for the diagnosis of equine infectious anemia (EIA) using recombinant protein produced from a synthetic p26 gene of EIA virus. Indian J Virol 2013 Dec;24(3):349-56.
- Costa VMD, de Aguiar Filho RN, Braz GF, Paz CFR, Drumond BP, Teixeira BM, Kroon EG. Equine infectious anemia in Brazilian equestrian tourism horses: serological, molecular, and biochemical assessment showing high EIAV seroprevalence and elevated gamma-glutamyl transferase (GGT) levels. Vet Res Commun 2025 Nov 27;50(1):52.
- Wang J, Qiu J, Wang M, Wu X, Li X, Zhang H. Development of a colloidal gold immunochromatographic strip to detect equine infectious anemia virus. Virol J 2025 Jun 24;22(1):205.
- Domínguez-Odio A, González LIC, Alfonso DM, Guevara-Hernández F, La O Arias MA, Zayas MP, Ríos MÁB. Serodiagnosis of equine infectious anemia by indirect ELISA based on a novel synthetic peptide derived from gp45 glycoprotein. Vet Res Commun 2025 Apr 22;49(3):174.
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