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Anatomical record (Hoboken, N.J. : 2007)2013; 296(7); 1115-1126; doi: 10.1002/ar.22709

Region-specific expression of aquaporin subtypes in equine testis, epididymis, and ductus deferens.

Abstract: The process of water movement in the excurrent duct system of the male reproductive tract is pivotal for establishment of male fertility. The objective was to elucidate expression of aquaporin (AQP) water channels in the stallion reproductive tract. Real-time RT-PCR detected expression of AQP0-5 and AQP7-11 in testis, epididymis, and ductus deferens of mature stallions. There were two main expression patterns: (1) higher expression in testis than in epididymis and ductus deferens (AQP0, -4, -5, -8, -10, and -11); and (2) lower expression in testis than in epididymis and ductus deferens (AQP1, -3, -7, and -9). Overall, we inferred that fluid transport in the stallion testicle involved a collaboration of AQP subtypes (primarily AQP2, -5, -7, and -8). Based on immunohistochemistry, expression of AQP subtypes analyzed (i.e., AQP0, -2, -5, and -9) was localized to Leydig cells and elongated and round spermatids. Functional significance of AQP expression by Leydig cells remained uncertain. In elongated and round spermatids, AQP s likely contributed to the volume reduction observed during spermatogenesis. Subtypes AQP2 and AQP9 were the predominant forms expressed in epididymal tissue. Regulation of AQP2 expression, especially in the epididymal head, seemed to occur at the post-transcriptional level, as protein expression upon immunohistochemistry was pronounced, despite low transcript abundance. In epididymal tissue, AQPs likely contributed to fluid resorbtion, given their localization on the apical membrane of principal cells.
Copyright © 2013 Wiley Periodicals, Inc.
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Publication Date: 2013-05-27 PubMed ID: 23712968DOI: 10.1002/ar.22709Google Scholar: Lookup
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  • Comparative Study
  • Journal Article
  • Research Support
  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research investigates the expression of aquaporin (AQP) water channels in the reproductive tract of stallions. In particular, it finds distinct expression patterns of different AQP subtypes in the testis, epididymis, and ductus deferens, suggesting complex regulation of fluid transport necessary for male fertility.

Introduction

  • The article explores the role of water movement in the excurrent duct system of the male reproductive tract, which is critical for male fertility.
  • The researchers examined the expression of aquaporin (AQP) water channels in the reproductive tract of stallions. These AQPs are proteins that facilitate the transport of water across cell membranes.

Data Collection

  • With the use of real-time RT-PCR, the study detected the expression of AQP subtypes 0 to 5 and 7 to 11 in the testis, epididymis, and ductus deferens of mature stallions.

Expression Patterns

  • The study found two main expression patterns: higher expression in testis than in epididymis and ductus deferens for certain AQP subtypes and lower expression in the testis for others.
  • The researchers inferred that fluid transport in the stallion testicle involved a collaborative action of different AQP subtypes, primarily AQP2, -5, -7, and -8.

Immunohistochemical Analysis

  • The researchers also carried out immunohistochemistry to locate the expressed AQP subtypes. Expression was found in Leydig cells and in both elongated and round spermatids.
  • The functional significance of AQP expression by Leydig cells remains uncertain. However, in elongated and round spermatids, it is likely that AQP expression aids in the volume reduction observed during spermatogenesis, a process where spermatozoa are produced.

Regulation of AQPs

  • In epididymal tissue, AQP2 and AQP9 were predominantly expressed.
  • The study also inferred that the regulation of AQP2 expression in the epididymal head occurred at the post-transcriptional level. Despite low transcript abundance, protein expression was pronounced upon immunohistochemistry. This suggests a possible regulation process for AQP synthesis.
  • Due to their localization on the apical membrane of principal cells in the epididymal tissue, it is proposed that AQPs contribute to fluid resorption during the process of sperm maturation.

This research illuminates the role of AQPs in fluid transport within the stallion’s reproductive tract, advancing our understanding of their contribution to male fertility. Yet, further investigation is needed to uncover the precise function of AQPs in the different stages of sperm production and maturation.

Cite This Article

APA
Klein C, Troedsson MH, Rutllant J. (2013). Region-specific expression of aquaporin subtypes in equine testis, epididymis, and ductus deferens. Anat Rec (Hoboken), 296(7), 1115-1126. https://doi.org/10.1002/ar.22709

Publication

Anatomical record (Hoboken, N.J. : 2007)
ISSN: 1932-8494
NlmUniqueID: 101292775
Country: United States
Language: English
Volume: 296
Issue: 7
Pages: 1115-1126

Researcher Affiliations

Klein, C
  • College of Veterinary Medicine, University of Calgary, Calgary, Alberta T2N 4Z6, Canada. claudia.klein@ucalgary.edu
Troedsson, M H T
    Rutllant, J

      MeSH Terms

      • Animals
      • Aquaporins / analysis
      • Aquaporins / genetics
      • Blotting, Western
      • Epididymis / chemistry
      • Gene Expression Regulation
      • Horses / genetics
      • Horses / metabolism
      • Immunohistochemistry
      • Leydig Cells / chemistry
      • Male
      • RNA, Messenger / analysis
      • Real-Time Polymerase Chain Reaction
      • Reverse Transcriptase Polymerase Chain Reaction
      • Spermatids / chemistry
      • Testis / chemistry
      • Vas Deferens / chemistry

      Citations

      This article has been cited 16 times.
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        doi: 10.3390/ijms241411770pubmed: 37511528google scholar: lookup
      2. Al-Thnaian TA. Morphological and Molecular Investigations of Aquaporin-7 (AQP-7) in Male Camelus dromedarius Reproductive Organs. Animals (Basel) 2023 Mar 25;13(7).
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