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Home / Equine Research Bank / Reprod Biol Endocrinol / Regulation of ACVR1 and ID2 by cell-secreted exosomes during...
Reproductive biology and endocrinology : RB&E2014; 12; 44; doi: 10.1186/1477-7827-12-44

Regulation of ACVR1 and ID2 by cell-secreted exosomes during follicle maturation in the mare.

Abstract: Ovarian follicle growth and maturation requires extensive communication between follicular somatic cells and oocytes. Recently, intercellular cell communication was described involving cell-secreted vesicles called exosomes (50-150 nm), which contain miRNAs and protein, and have been identified in ovarian follicular fluid. The goal of this study was to identify a possible role of exosomes in follicle maturation. Methods: Follicle contents were collected from mares at mid-estrous (~35 mm, before induction of follicular maturation) and pre-ovulatory follicles (30-34 h after induction of follicular maturation). A real time PCR screen was conducted to reveal significant differences in the presence of exosomal miRNAs isolated from mid-estrous and pre-ovulatory follicles, and according to bioinformatics analysis these exosomal miRNAs are predicted to target members belonging to the TGFB superfamily, including ACVR1 and ID2. Granulosa cells from pre-ovulatory follicles were cultured and treated with exosomes isolated from follicular fluid. Changes in mRNA and protein were measured by real time PCR and Western blot. Results: ACVR1 mRNA and protein was detected in granulosa cells at mid-estrous and pre-ovulatory stages, and real time PCR analysis revealed significantly lower levels of ID2 (an ACVR1 target gene) in granulosa cells from pre-ovulatory follicles. Exposure to exosomes from follicular fluid of mid-estrous follicles decreased ID2 levels in granulosa cells. Moreover, exosomes isolated from mid-estrous and pre-ovulatory follicles contain ACVR1 and miR-27b, miR-372, and miR-382 (predicted regulators of ACVR1 and ID2) were capable of altering ID2 levels in pre-ovulatory granulosa cells. Conclusions: These data indicate that exosomes isolated from follicular fluid can regulate members of the TGFB/BMP signaling pathway in granulosa cells, and possibly play a role in regulating follicle maturation.
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Publication Date: 2014-05-26 PubMed ID: 24884710PubMed Central: PMC4045866DOI: 10.1186/1477-7827-12-44Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research article reveals the regulatory role of cell-secreted exosomes in ovarian follicle maturation in mares. Specifically, the exosomes, which are small vesicles containing microRNAs and proteins, target the TGFB superfamily members ACVR1 and ID2, affecting changes in their mRNA and protein levels during follicular maturation stages.

Research Methods

  • The primary methodological approach was the collection of follicle contents from mares at mid-estrous. This is a stage before the induction of follicular maturation, and pre-ovulatory follicles, which is 30-34 hours after the induction of follicular maturation.
  • Through a real time PCR screen, the researchers were able to identify significant differences in exosomal microRNAs both at mid-estrous and pre-ovulatory stages.
  • The study included a bioinformatics analysis which determined that these microRNAs target ACVR1 and ID2 of the TGFB superfamily.
  • An additional part of the research involved culturing granulosa cells from pre-ovulatory follicles and then treating these cells with exosomes isolated from follicular fluid. The changes in mRNA and protein were noted via real time PCR and Western blot.

Research Findings

  • In the granulosa cells at both mid-estrous and pre-ovulatory stages, ACVR1 mRNA and protein were detected.
  • Through real time PCR analysis, the researchers discovered significantly lower levels of ID2 (a target gene of ACVR1) in granulosa cells from pre-ovulatory follicles.
  • Exposure to exosomes from follicular fluid of mid-estrous follicles decreased ID2 levels in granulosa cells.
  • The experiment verified that the exosomes, which contain ACVR1 and microRNA-27b, microRNA-372, and microRNA-382 (predicted regulators of ACVR1 and ID2), were capable of modifying ID2 levels in pre-ovulatory granulosa cells.

Conclusions

  • Overall, the data from the research indicates that exosomes isolated from follicular fluid can regulate ACVR1 and ID2, members of the TGFB/BMP signaling pathway, in granulosa cells.
  • This study suggests a potential role of exosomes in the intricate process of ovular follicle maturation. This could have profound implications on understanding the complexities of reproductive biology and potentially advancing reproductive technologies.

Cite This Article

APA
da Silveira JC, Carnevale EM, Winger QA, Bouma GJ. (2014). Regulation of ACVR1 and ID2 by cell-secreted exosomes during follicle maturation in the mare. Reprod Biol Endocrinol, 12, 44. https://doi.org/10.1186/1477-7827-12-44

Publication

Reproductive biology and endocrinology : RB&E
ISSN: 1477-7827
NlmUniqueID: 101153627
Country: England
Language: English
Volume: 12
Pages: 44

Researcher Affiliations

da Silveira, Juliano C
    Carnevale, Elaine M
      Winger, Quinton A
        Bouma, Gerrit J
        • Animal Reproduction and Biotechnology Laboratory, Department of Biomedical Sciences, Colorado State University, Fort Collins, CO, USA. Gerrit.Bouma@colostate.edu.

        MeSH Terms

        • Activin Receptors, Type I / antagonists & inhibitors
        • Activin Receptors, Type I / genetics
        • Activin Receptors, Type I / metabolism
        • Animals
        • Cells, Cultured
        • Colorado
        • Computational Biology / methods
        • Estrous Cycle / metabolism
        • Exocytosis
        • Exosomes / metabolism
        • Female
        • Follicular Fluid / cytology
        • Follicular Fluid / metabolism
        • Gene Expression Regulation, Developmental
        • Granulosa Cells / cytology
        • Granulosa Cells / metabolism
        • Horses / physiology
        • Inhibitor of Differentiation Protein 2 / antagonists & inhibitors
        • Inhibitor of Differentiation Protein 2 / genetics
        • Inhibitor of Differentiation Protein 2 / metabolism
        • MicroRNAs / metabolism
        • Oogenesis
        • Ovarian Follicle / cytology
        • Ovarian Follicle / metabolism
        • RNA, Messenger / metabolism
        • Signal Transduction

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