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Home / Equine Research Bank / Virology / Regulation of equine herpesvirus type 1 gene expression: cha...
Virology1987; 158(1); 79-87; doi: 10.1016/0042-6822(87)90240-6

Regulation of equine herpesvirus type 1 gene expression: characterization of immediate early, early, and late transcription.

Abstract: The regulation of equine herpesvirus type 1 (EHV-1) transcription was examined in infected rabbit kidney cells using metabolic inhibitors. In order to map EHV-1 immediate early, early, and late transcripts, viral RNA was 32P-labeled in vivo and hybridized to EHV-1 DNA restriction fragments immobilized on nitrocellulose filters. Immediate early viral RNA was mapped to one region of the viral genome within the inverted repeat DNA sequences (map units 0.78-0.83 and 0.95-1.0). Northern blot hybridization analysis using a 32P-labeled cloned DNA probe from this region identified a single immediate early viral transcript (approximately 6 kb). Transcription of early and late genes was not restricted to any specific region on the viral genome as indicated by the ability of 32P-labeled early and late RNA to hybridize to EHV-1 restriction endonuclease fragments from both the long and short components of EHV-1 DNA. Additional experiments performed without the use of metabolic inhibitors confirmed that EHV-1 transcription is temporally regulated. The characterization of EHV-1 transcription during productive infection will serve as a reference for the analysis of viral transcripts in oncogenically transformed and persistently infected cells.
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Publication Date: 1987-05-01 PubMed ID: 3033896DOI: 10.1016/0042-6822(87)90240-6Google Scholar: Lookup
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  • Journal Article
  • Research Support
  • Non-U.S. Gov't
  • Research Support
  • U.S. Gov't
  • P.H.S.

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The researchers studied how the transcription of equine herpesvirus type 1 (EHV-1) is regulated in infected rabbit kidney cells. They found that transcription of immediate early, early, and late genes occurred in different areas of the virus’s genome and at different times.

Mapping EHV-1 Transcripts

  • The study used metabolic inhibitors to examine the transcription of EHV-1 in infected rabbit kidney cells.
  • To identify and map the immediate early, early, and late transcripts of the virus, researchers labeled the viral RNA in vivo, which they later hybridized to EHV-1 DNA restriction fragments.
  • The research found that immediate early viral RNA was located in a specific region of the viral genome, specifically within the inverted repeat DNA sequences.

Transcription of Early and Late Genes

  • The analysis revealed that transcription of early and late genes did not occur in any specific region on the virus genome.
  • The evidence for this observation was that labeled early and late RNA was able to bind with EHV-1 DNA restriction fragments from both the long and short components of the virus’s DNA.

Temporal Regulation of EHV-1 Transcription

  • Additional experiments were conducted without the use of metabolic inhibitors.
  • These confirmed that EHV-1 transcription is regulated temporally. In simpler terms, this means that different parts of the virus’s genome are transcribed at different stages of the infection.
  • This temporal regulation was characterized in more detail during the study for future research use, potentially for the analysis of viral transcripts in oncogenically transformed and persistently infected cells.

The paper concludes by indicating the importance of how understanding how the EHV-1 is regulated during different stages of infection can provide valuable information for future virus and oncogenic research.

Cite This Article

APA
Gray WL, Baumann RP, Robertson AT, Caughman GB, O'Callaghan DJ, Staczek J. (1987). Regulation of equine herpesvirus type 1 gene expression: characterization of immediate early, early, and late transcription. Virology, 158(1), 79-87. https://doi.org/10.1016/0042-6822(87)90240-6

Publication

Virology
ISSN: 0042-6822
NlmUniqueID: 0110674
Country: United States
Language: English
Volume: 158
Issue: 1
Pages: 79-87

Researcher Affiliations

Gray, W L
    Baumann, R P
      Robertson, A T
        Caughman, G B
          O'Callaghan, D J
            Staczek, J

              MeSH Terms

              • Animals
              • Cells, Cultured
              • Cycloheximide / pharmacology
              • DNA Restriction Enzymes
              • DNA, Viral / genetics
              • Gene Expression Regulation
              • Genes, Viral
              • Herpesviridae / genetics
              • Herpesvirus 1, Equid / genetics
              • Herpesvirus 1, Equid / physiology
              • Nucleic Acid Hybridization
              • RNA, Viral / genetics
              • Transcription, Genetic

              Grant Funding

              • AI21996 / NIAID NIH HHS
              • AI22001 / NIAID NIH HHS
              • AI22894 / NIAID NIH HHS

              Citations

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