Regulation of matrix metabolism in equine cartilage explant cultures by interleukin 1.
Abstract: Explant cultures were set up, using articular cartilage obtained from metatarsophalangeal joints of 11 horses. Explants from 2 horses were used to determine culture conditions appropriate for tissue viability. The cartilage explants maintained steady-state metabolism of proteoglycans during a 13-day evaluation period. The metabolic response of equine articular cartilage to incubation with recombinant human interleukin 1 (0.01 to 100 ng/ml) was studied, using cartilage obtained from the remaining 9 horses, age of which ranged from 3 months to 20 years. Interleukin 1 induced a dose-dependent release of glycosaminoglycan from the matrix during a 3-day incubation period. It also caused dose-dependent inhibition of glycosaminoglycan synthesis during a 3-hour pulse-labeling period. Explants obtained from older horses were significantly (P < 0.05) less responsive to interleukin 1, with respect to synthesis and release of glycosaminoglycan.
Publication Date: 1992-12-01 PubMed ID: 1476308
The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.
This research investigates how the protein Interleukin 1 influences the metabolism of the matrix in horse cartilage. The study indicates that the protein promotes the release and inhibits the creation of glycosaminoglycan, a compound found in cartilage, while noting that older horses show less responsiveness.
Investigating Cartilage Metabolism
- The research involved studying explant cultures, which are samples of living tissue removed from an organism and placed in an artificial environment for investigation. The researchers obtained articular cartilage from the metatarsophalangeal joints (the joint of the foot where the toe bones meet the metatarsal or long bone of the foot) of 11 horses.
- Two horses’ explants were used to determine the optimal conditions for keeping the tissue viable (alive and functioning as normal) in the artificial culture environment.
- The cartilage samples maintained a steady metabolism of proteoglycans, a type of molecule heavily involved in the structure and function of cartilage, throughout a 13-day evaluation period.
Effects of Interleukin 1
- The key aspect of the research was to explore the metabolic response of horse cartilage to a protein called Interleukin 1. The researchers did this by incubating the cartilage samples with varying doses of this protein.
- Interleukin 1 triggered a release of glycosaminoglycans (GAGs) from the matrix, the innermost component of the cartilage where the cells reside, over a 3-day period. Glycosaminoglycan is an essential compound found in cartilage that helps to resist compressive forces.
- In addition, Interleukin 1 caused an inhibition of GAG synthesis during a brief (3-hour) period, suggesting that it slows down the production of this key cartilage component.
Age-Related Differences
- The research also compared the effects of Interleukin 1 on horses of different ages, ranging from 3 months to 20 years old.
- The study found that cartilage explants from older horses showed significantly less responsiveness to Interleukin 1. This was evident in the synthesis and release of GAGs, implying that the metabolism of cartilage matrix changes as horses age.
Cite This Article
APA
MacDonald MH, Stover SM, Willits NH, Benton HP.
(1992).
Regulation of matrix metabolism in equine cartilage explant cultures by interleukin 1.
Am J Vet Res, 53(12), 2278-2285.
Publication
Researcher Affiliations
- Department of Veterinary Anatomy, University of California, Davis 95616.
MeSH Terms
- Animals
- Cartilage, Articular / metabolism
- Culture Techniques
- Extracellular Matrix / metabolism
- Female
- Glycosaminoglycans / biosynthesis
- Glycosaminoglycans / metabolism
- Horses / metabolism
- Interleukin-1 / physiology
- Male
Citations
This article has been cited 4 times.- Peffers MJ, Beynon RJ, Clegg PD. Absolute quantification of selected proteins in the human osteoarthritic secretome.. Int J Mol Sci 2013 Oct 15;14(10):20658-81.
- Li KW, Siraj SA, Cheng EW, Awada M, Hellerstein MK, Turner SM. A stable isotope method for the simultaneous measurement of matrix synthesis and cell proliferation in articular cartilage in vivo.. Osteoarthritis Cartilage 2009 Jul;17(7):923-32.
- Tung JT, Fenton JI, Arnold C, Alexander L, Yuzbasiyan-Gurkan V, Venta PJ, Peters TL, Orth MW, Richardson DW, Caron JP. Recombinant equine interleukin-1beta induces putative mediators of articular cartilage degradation in equine chondrocytes.. Can J Vet Res 2002 Jan;66(1):19-25.
- Little CB, Flannery CR, Hughes CE, Mort JS, Roughley PJ, Dent C, Caterson B. Aggrecanase versus matrix metalloproteinases in the catabolism of the interglobular domain of aggrecan in vitro.. Biochem J 1999 Nov 15;344 Pt 1(Pt 1):61-8.
Use Nutrition Calculator
Check if your horse's diet meets their nutrition requirements with our easy-to-use tool Check your horse's diet with our easy-to-use tool
Talk to a Nutritionist
Discuss your horse's feeding plan with our experts over a free phone consultation Discuss your horse's diet over a phone consultation
Submit Diet Evaluation
Get a customized feeding plan for your horse formulated by our equine nutritionists Get a custom feeding plan formulated by our nutritionists
