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Journal of virology1995; 69(5); 2786-2793; doi: 10.1128/JVI.69.5.2786-2793.1995

Regulatory function of the equine herpesvirus 1 ICP27 gene product.

Abstract: The UL3 protein of equine herpesvirus 1 (EHV-1) KyA strain is a homolog of the ICP27 alpha regulatory protein of herpes simplex virus type 1 (HSV-1) and the ORF 4 protein of varicella-zoster virus. To characterize the regulatory function of the UL3 gene product, a UL3 gene expression vector (pSVUL3) and a vector expressing a truncated version of the UL3 gene (pSVUL3P) were generated. These effector plasmids, in combination with an EHV-1 immediate-early (IE) gene expression vector (pSVIE) and chimeric EHV-1 promoter-chloramphenicol acetyltransferase (CAT) reporter constructs, were used in transient transfection assays. These assays demonstrated that the EHV-1 UL3 gene product is a regulatory protein that can independently trans activate the EHV-1 IE promoter; however, this effect can be inhibited by the repressive function of the IE gene product on the IE promoter (R. H. Smith, G. B. Caughman, and D. J. O'Callaghan, J. Virol. 66:936-945, 1992). In the presence of the IE gene product, the UL3 gene product significantly augments gene expression directed by the promoters of three EHV-1 early genes (thymidine kinase; IR4, which is the homolog of HSV-1 ICP22; and UL3 [ICP27]) and the promoter of the EHV-1 late gene IR5, which is the homolog of HSV-1 US10. Sequences located at nucleotides -123 to +20 of the UL3 promoter harbor a TATA box, SP1 binding site, CAAT box, and octamer binding site and, when linked to the CAT reporter gene, are trans activated to maximal levels by the pSVIE construct in transient expression assays. Results from CAT assays also suggest that the first 11 amino acids of the UL3 protein are not essential for the regulatory function of the UL3 gene product.
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Publication Date: 1995-05-01 PubMed ID: 7707500PubMed Central: PMC188972DOI: 10.1128/JVI.69.5.2786-2793.1995Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research discusses the regulatory function of the UL3 gene product related to equine herpesvirus 1 (EHV-1), a horse virus. The scientists made and tested vectors expressing the UL3 gene and a truncated version to delineate its role in various gene expressions, and found the UL3 gene product capable of independent activation and regulation in the context of other gene promoters.

Regulatory Role of UL3 Gene Product

  • The researchers focused on the UL3 protein of EHV-1, which is similar to the ICP27 alpha regulatory protein of herpes simplex virus type 1 (HSV-1) and the ORF 4 protein of varicella-zoster virus.
  • They generated two vectors – one expressing the gene pSVUL3 and another expressing a truncated version of this gene, pSVUL3P.
  • The vectors were paired with an EHV-1 immediate-early (IE) gene expression vector (pSVIE) and tested using EHV-1 promoter-chloramphenicol acetyltransferase (CAT) reporter constructs in transient transfection assays.
  • The assays showed that the UL3 gene product can independently trans activate EHV-1 IE promoter marking its regulatory function. However, this function is repressed by the IE gene.

Enhanced Gene Expression

  • When the UL3 gene product is present with the IE gene product, it can significantly augment gene expression guided by EHV-1 early gene promoters and the promoter of the EHV-1 late gene IR5.

Sequences of UL3 Promoter

  • Researchers identified the sequences in the UL3 promoter which possess a TATA box, SP1 binding site, CAAT box, and octamer binding site. These sequences, when attached to the CAT reporter gene, are trans activated to maximum levels by the pSVIE construct.
  • CAT assays further imply that the first 11 amino acids of the UL3 protein are not crucial for the regulatory function of the UL3 gene product.

The detailed examination of the UL3 gene product informs about its crucial role in gene regulation in the context of EHV-1 and has potential implications in understanding and treating related viral infections.

Cite This Article

APA
Zhao Y, Holden VR, Smith RH, O'Callaghan DJ. (1995). Regulatory function of the equine herpesvirus 1 ICP27 gene product. J Virol, 69(5), 2786-2793. https://doi.org/10.1128/JVI.69.5.2786-2793.1995

Publication

Journal of virology
ISSN: 0022-538X
NlmUniqueID: 0113724
Country: United States
Language: English
Volume: 69
Issue: 5
Pages: 2786-2793

Researcher Affiliations

Zhao, Y
  • Department of Microbiology and Immunology, Louisiana State University Medical Center, Shreveport 71130-3932, USA.
Holden, V R
    Smith, R H
      O'Callaghan, D J

        MeSH Terms

        • Animals
        • Antibodies, Viral / biosynthesis
        • Base Sequence
        • Cell Line
        • DNA Primers / genetics
        • DNA, Viral / genetics
        • Gene Expression
        • Genes, Viral
        • Genetic Vectors
        • Herpesvirus 1, Equid / genetics
        • Herpesvirus 1, Equid / physiology
        • Immediate-Early Proteins
        • Molecular Sequence Data
        • Promoter Regions, Genetic
        • Transcriptional Activation
        • Viral Proteins / genetics
        • Viral Proteins / physiology

        Grant Funding

        • AI 22001 / NIAID NIH HHS

        References

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